1OYG

Crystal structure of Bacillus subtilis levansucrase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0009758	biological_process	carbohydrate utilization
A	0016740	molecular_function	transferase activity
A	0016757	molecular_function	glycosyltransferase activity
A	0046872	molecular_function	metal ion binding
A	0050053	molecular_function	levansucrase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 500

Chain	Residue
A	ASP241
A	GLN272
A	LEU308
A	ASN310
A	ASP339
A	HOH532

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 501

Chain	Residue
A	EDO502
A	HOH584
A	ASP94
A	ALA151
A	ASN152

---

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EDO A 502

Chain	Residue
A	ASP94
A	ASN152
A	GLU252
A	ASP253
A	LYS348
A	EDO501
A	HOH525
A	HOH578
A	HOH850

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 503

Chain	Residue
A	HIS104
A	GLN126
A	GLY129
A	GLU130
A	THR470
A	ASN472
A	HOH572

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 504

Chain	Residue
A	ASN242
A	ARG246
A	GLU340
A	LYS363

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 505

Chain	Residue
A	ASN450
A	LYS452
A	LYS455
A	THR456
A	SER457

---

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EDO A 506

Chain	Residue
A	GLN229
A	ALA419
A	LYS420
A	VAL471
A	ASN472
A	HOH635
A	HOH659
A	HOH792
A	HOH835

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 507

Chain	Residue
A	PHE169
A	ILE217
A	THR323
A	LEU324
A	HOH681
A	HOH871

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1PT2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BYN","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18596022","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32553967","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"33303628","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1OYG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PT2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VDT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BYJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BYK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BYN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6PWQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6VHQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	a catalytic site defined by CSA, PubMed 14517548

Chain	Residue	Details
A	GLU342
A	ASP86
A	ASP247

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 875

Chain	Residue	Details
A	ASP86	covalent catalysis
A	SER164	steric locator
A	ASP247	electrostatic stabiliser
A	GLU342	proton shuttle (general acid/base)

---