1O2F
COMPLEX OF ENZYME IIAGLC AND IIBGLC PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0009401 | biological_process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
| A | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| A | 0016020 | cellular_component | membrane |
| A | 0016301 | molecular_function | kinase activity |
| A | 0034763 | biological_process | negative regulation of transmembrane transport |
| A | 0043610 | biological_process | regulation of carbohydrate utilization |
| A | 0045912 | biological_process | negative regulation of carbohydrate metabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 1902344 | biological_process | negative regulation of maltose transport |
| A | 1902495 | cellular_component | transmembrane transporter complex |
| A | 1990154 | cellular_component | enzyme IIA-maltose transporter complex |
| B | 0008982 | molecular_function | protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
| B | 0009401 | biological_process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Functional Information from PROSITE/UniProt
| site_id | PS00371 |
| Number of Residues | 13 |
| Details | PTS_EIIA_TYPE_1_HIS PTS EIIA domains phosphorylation site signature 1. GveLFVHFGIdTV |
| Chain | Residue | Details |
| A | GLY84-VAL96 |
| site_id | PS01035 |
| Number of Residues | 18 |
| Details | PTS_EIIB_TYPE_1_CYS PTS EIIB domains cysteine phosphorylation site signature. NItnldaCiTRLrVsVaD |
| Chain | Residue | Details |
| B | ASN328-ASP345 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Phosphocysteine intermediate; for EIIB activity => ECO:0000255|PROSITE-ProRule:PRU00421, ECO:0000305|PubMed:12716891, ECO:0000305|PubMed:18319344, ECO:0000305|PubMed:3129430 |
| Chain | Residue | Details |
| B | CYS335 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphocysteine => ECO:0000269|PubMed:8505292 |
| Chain | Residue | Details |
| B | CYS335 | |
| A | PHE91 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | SITE: Important for phospho-donor activity => ECO:0000269|PubMed:2657735 |
| Chain | Residue | Details |
| A | ALA76 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphohistidine; by HPr => ECO:0000269|PubMed:2657735 |
| Chain | Residue | Details |
| A | PHE91 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1gpr |
| Chain | Residue | Details |
| A | HIS90 | |
| A | HIS75 | |
| A | GLY92 | |
| A | THR73 |
