1O2F

COMPLEX OF ENZYME IIAGLC AND IIBGLC PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
A	0009898	cellular_component	cytoplasmic side of plasma membrane
A	0016020	cellular_component	membrane
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0034763	biological_process	negative regulation of transmembrane transport
A	0043610	biological_process	regulation of carbohydrate utilization
A	0045912	biological_process	negative regulation of carbohydrate metabolic process
A	0046872	molecular_function	metal ion binding
A	1902344	biological_process	negative regulation of maltose transport
A	1902495	cellular_component	transmembrane transporter complex
A	1990154	cellular_component	enzyme IIA-maltose transporter complex
B	0008982	molecular_function	protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
B	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system

Functional Information from PROSITE/UniProt

site_id	PS00371
Number of Residues	13
Details	PTS_EIIA_TYPE_1_HIS PTS EIIA domains phosphorylation site signature 1. GveLFVHFGIdTV

Chain	Residue	Details
A	GLY84-VAL96

site_id	PS01035
Number of Residues	18
Details	PTS_EIIB_TYPE_1_CYS PTS EIIB domains cysteine phosphorylation site signature. NItnldaCiTRLrVsVaD

Chain	Residue	Details
B	ASN328-ASP345

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	104
Details	Domain: {"description":"PTS EIIA type-1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00416","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Tele-phosphohistidine intermediate; for EIIA activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00416","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"2657735","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"6383826","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"1961703","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9405042","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"2657735","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8170944","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9405042","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"1961703","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	1
Details	Site: {"description":"Important for phospho-donor activity","evidences":[{"source":"PubMed","id":"2657735","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphohistidine; by HPr","evidences":[{"source":"PubMed","id":"2657735","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	1
Details	Active site: {"description":"Phosphocysteine intermediate; for EIIB activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00421","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12716891","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18319344","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"3129430","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphocysteine","evidences":[{"source":"PubMed","id":"8505292","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1gpr