1O0S
Crystal Structure of Ascaris suum Malic Enzyme Complexed with NADH
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004470 | molecular_function | malic enzyme activity |
| A | 0004471 | molecular_function | malate dehydrogenase (decarboxylating) (NAD+) activity |
| A | 0004473 | molecular_function | malate dehydrogenase (decarboxylating) (NADP+) activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0006108 | biological_process | malate metabolic process |
| A | 0008948 | molecular_function | oxaloacetate decarboxylase activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051287 | molecular_function | NAD binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004470 | molecular_function | malic enzyme activity |
| B | 0004471 | molecular_function | malate dehydrogenase (decarboxylating) (NAD+) activity |
| B | 0004473 | molecular_function | malate dehydrogenase (decarboxylating) (NADP+) activity |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0006108 | biological_process | malate metabolic process |
| B | 0008948 | molecular_function | oxaloacetate decarboxylase activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051287 | molecular_function | NAD binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE TTN A 810 |
| Chain | Residue |
| A | GLN78 |
| A | ARG81 |
| A | VAL82 |
| A | ARG105 |
| A | LEU109 |
| A | HOH943 |
| A | HOH952 |
| B | TYR141 |
| B | HOH954 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE TTN B 815 |
| Chain | Residue |
| A | TYR141 |
| B | GLN78 |
| B | ARG81 |
| B | VAL82 |
| B | ARG105 |
| B | HOH937 |
| B | HOH964 |
| site_id | AC3 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE NAI A 920 |
| Chain | Residue |
| A | ARG181 |
| A | LEU183 |
| A | ASN275 |
| A | ASP295 |
| A | THR299 |
| A | PHE326 |
| A | GLY327 |
| A | ALA328 |
| A | GLY329 |
| A | ALA330 |
| A | ALA331 |
| A | ASP361 |
| A | ILE362 |
| A | ALA405 |
| A | THR407 |
| A | VAL408 |
| A | LEU432 |
| A | SER433 |
| A | ASN434 |
| A | HOH964 |
| A | HOH1057 |
| A | HOH1149 |
| A | HOH1161 |
| site_id | AC4 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE NAI B 930 |
| Chain | Residue |
| B | ARG181 |
| B | LEU183 |
| B | ASN275 |
| B | ASP295 |
| B | THR299 |
| B | PHE326 |
| B | GLY327 |
| B | ALA328 |
| B | GLY329 |
| B | ALA330 |
| B | ALA331 |
| B | ASP361 |
| B | ILE362 |
| B | ALA405 |
| B | VAL408 |
| B | LEU432 |
| B | SER433 |
| B | ASN434 |
| B | HOH942 |
| B | HOH981 |
| B | HOH1065 |
| B | HOH1092 |
Functional Information from PROSITE/UniProt
| site_id | PS00331 |
| Number of Residues | 17 |
| Details | MALIC_ENZYMES Malic enzymes signature. FnDDiqGTAsViVAGLL |
| Chain | Residue | Details |
| A | PHE292-LEU308 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor |
| Chain | Residue | Details |
| A | TYR126 | |
| B | TYR126 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| A | LYS199 | |
| B | LYS199 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:12853453 |
| Chain | Residue | Details |
| A | GLN78 | |
| A | ARG81 | |
| A | ARG105 | |
| B | GLN78 | |
| B | ARG81 | |
| B | ARG105 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:12853453, ECO:0007744|PDB:1O0S |
| Chain | Residue | Details |
| A | ARG181 | |
| A | ASN275 | |
| A | ASP295 | |
| B | ARG181 | |
| B | ASN275 | |
| B | ASP295 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P23368 |
| Chain | Residue | Details |
| A | GLU271 | |
| A | ASP272 | |
| A | ASN478 | |
| B | GLU271 | |
| B | ASP272 | |
| B | ASN478 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12033925, ECO:0000305|PubMed:12853453, ECO:0007744|PDB:1LLQ, ECO:0007744|PDB:1O0S |
| Chain | Residue | Details |
| A | ALA328 | |
| A | ALA331 | |
| B | ALA328 | |
| B | ALA331 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12033925, ECO:0000269|PubMed:12853453, ECO:0007744|PDB:1LLQ, ECO:0007744|PDB:1O0S |
| Chain | Residue | Details |
| A | ASN434 | |
| B | ASN434 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1do8 |
| Chain | Residue | Details |
| A | LYS199 | |
| A | TYR126 | |
| A | ASP294 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1do8 |
| Chain | Residue | Details |
| B | LYS199 | |
| B | TYR126 | |
| B | ASP294 |
