1NSK

THE CRYSTAL STRUCTURE OF A HUMAN NUCLEOSIDE DIPHOSPHATE KINASE, NM23-H2

Functional Information from GO Data

Chain	GOid	namespace	contents
L	0000166	molecular_function	nucleotide binding
L	0001216	molecular_function	DNA-binding transcription activator activity
L	0001726	cellular_component	ruffle
L	0003677	molecular_function	DNA binding
L	0004518	molecular_function	nuclease activity
L	0004550	molecular_function	nucleoside diphosphate kinase activity
L	0004673	molecular_function	protein histidine kinase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005576	cellular_component	extracellular region
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005925	cellular_component	focal adhesion
L	0006183	biological_process	GTP biosynthetic process
L	0006228	biological_process	UTP biosynthetic process
L	0006241	biological_process	CTP biosynthetic process
L	0007155	biological_process	cell adhesion
L	0007229	biological_process	integrin-mediated signaling pathway
L	0009117	biological_process	nucleotide metabolic process
L	0009142	biological_process	nucleoside triphosphate biosynthetic process
L	0016301	molecular_function	kinase activity
L	0016740	molecular_function	transferase activity
L	0019003	molecular_function	GDP binding
L	0030027	cellular_component	lamellipodium
L	0034774	cellular_component	secretory granule lumen
L	0042304	biological_process	regulation of fatty acid biosynthetic process
L	0042802	molecular_function	identical protein binding
L	0042981	biological_process	regulation of apoptotic process
L	0043066	biological_process	negative regulation of apoptotic process
L	0045618	biological_process	positive regulation of keratinocyte differentiation
L	0045682	biological_process	regulation of epidermis development
L	0045893	biological_process	positive regulation of DNA-templated transcription
L	0045944	biological_process	positive regulation of transcription by RNA polymerase II
L	0046042	biological_process	ITP biosynthetic process
L	0046872	molecular_function	metal ion binding
L	0048471	cellular_component	perinuclear region of cytoplasm
L	0050679	biological_process	positive regulation of epithelial cell proliferation
L	0051880	molecular_function	G-quadruplex DNA binding
L	0070062	cellular_component	extracellular exosome
L	0071944	cellular_component	cell periphery
L	0120225	molecular_function	coenzyme A binding
L	1904813	cellular_component	ficolin-1-rich granule lumen
L	2000144	biological_process	positive regulation of DNA-templated transcription initiation
N	0000166	molecular_function	nucleotide binding
N	0001216	molecular_function	DNA-binding transcription activator activity
N	0001726	cellular_component	ruffle
N	0003677	molecular_function	DNA binding
N	0004518	molecular_function	nuclease activity
N	0004550	molecular_function	nucleoside diphosphate kinase activity
N	0004673	molecular_function	protein histidine kinase activity
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005576	cellular_component	extracellular region
N	0005634	cellular_component	nucleus
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005925	cellular_component	focal adhesion
N	0006183	biological_process	GTP biosynthetic process
N	0006228	biological_process	UTP biosynthetic process
N	0006241	biological_process	CTP biosynthetic process
N	0007155	biological_process	cell adhesion
N	0007229	biological_process	integrin-mediated signaling pathway
N	0009117	biological_process	nucleotide metabolic process
N	0009142	biological_process	nucleoside triphosphate biosynthetic process
N	0016301	molecular_function	kinase activity
N	0016740	molecular_function	transferase activity
N	0019003	molecular_function	GDP binding
N	0030027	cellular_component	lamellipodium
N	0034774	cellular_component	secretory granule lumen
N	0042304	biological_process	regulation of fatty acid biosynthetic process
N	0042802	molecular_function	identical protein binding
N	0042981	biological_process	regulation of apoptotic process
N	0043066	biological_process	negative regulation of apoptotic process
N	0045618	biological_process	positive regulation of keratinocyte differentiation
N	0045682	biological_process	regulation of epidermis development
N	0045893	biological_process	positive regulation of DNA-templated transcription
N	0045944	biological_process	positive regulation of transcription by RNA polymerase II
N	0046042	biological_process	ITP biosynthetic process
N	0046872	molecular_function	metal ion binding
N	0048471	cellular_component	perinuclear region of cytoplasm
N	0050679	biological_process	positive regulation of epithelial cell proliferation
N	0051880	molecular_function	G-quadruplex DNA binding
N	0070062	cellular_component	extracellular exosome
N	0071944	cellular_component	cell periphery
N	0120225	molecular_function	coenzyme A binding
N	1904813	cellular_component	ficolin-1-rich granule lumen
N	2000144	biological_process	positive regulation of DNA-templated transcription initiation
O	0000166	molecular_function	nucleotide binding
O	0001216	molecular_function	DNA-binding transcription activator activity
O	0001726	cellular_component	ruffle
O	0003677	molecular_function	DNA binding
O	0004518	molecular_function	nuclease activity
O	0004550	molecular_function	nucleoside diphosphate kinase activity
O	0004673	molecular_function	protein histidine kinase activity
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005576	cellular_component	extracellular region
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005925	cellular_component	focal adhesion
O	0006183	biological_process	GTP biosynthetic process
O	0006228	biological_process	UTP biosynthetic process
O	0006241	biological_process	CTP biosynthetic process
O	0007155	biological_process	cell adhesion
O	0007229	biological_process	integrin-mediated signaling pathway
O	0009117	biological_process	nucleotide metabolic process
O	0009142	biological_process	nucleoside triphosphate biosynthetic process
O	0016301	molecular_function	kinase activity
O	0016740	molecular_function	transferase activity
O	0019003	molecular_function	GDP binding
O	0030027	cellular_component	lamellipodium
O	0034774	cellular_component	secretory granule lumen
O	0042304	biological_process	regulation of fatty acid biosynthetic process
O	0042802	molecular_function	identical protein binding
O	0042981	biological_process	regulation of apoptotic process
O	0043066	biological_process	negative regulation of apoptotic process
O	0045618	biological_process	positive regulation of keratinocyte differentiation
O	0045682	biological_process	regulation of epidermis development
O	0045893	biological_process	positive regulation of DNA-templated transcription
O	0045944	biological_process	positive regulation of transcription by RNA polymerase II
O	0046042	biological_process	ITP biosynthetic process
O	0046872	molecular_function	metal ion binding
O	0048471	cellular_component	perinuclear region of cytoplasm
O	0050679	biological_process	positive regulation of epithelial cell proliferation
O	0051880	molecular_function	G-quadruplex DNA binding
O	0070062	cellular_component	extracellular exosome
O	0071944	cellular_component	cell periphery
O	0120225	molecular_function	coenzyme A binding
O	1904813	cellular_component	ficolin-1-rich granule lumen
O	2000144	biological_process	positive regulation of DNA-templated transcription initiation
R	0000166	molecular_function	nucleotide binding
R	0001216	molecular_function	DNA-binding transcription activator activity
R	0001726	cellular_component	ruffle
R	0003677	molecular_function	DNA binding
R	0004518	molecular_function	nuclease activity
R	0004550	molecular_function	nucleoside diphosphate kinase activity
R	0004673	molecular_function	protein histidine kinase activity
R	0005515	molecular_function	protein binding
R	0005524	molecular_function	ATP binding
R	0005576	cellular_component	extracellular region
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005925	cellular_component	focal adhesion
R	0006183	biological_process	GTP biosynthetic process
R	0006228	biological_process	UTP biosynthetic process
R	0006241	biological_process	CTP biosynthetic process
R	0007155	biological_process	cell adhesion
R	0007229	biological_process	integrin-mediated signaling pathway
R	0009117	biological_process	nucleotide metabolic process
R	0009142	biological_process	nucleoside triphosphate biosynthetic process
R	0016301	molecular_function	kinase activity
R	0016740	molecular_function	transferase activity
R	0019003	molecular_function	GDP binding
R	0030027	cellular_component	lamellipodium
R	0034774	cellular_component	secretory granule lumen
R	0042304	biological_process	regulation of fatty acid biosynthetic process
R	0042802	molecular_function	identical protein binding
R	0042981	biological_process	regulation of apoptotic process
R	0043066	biological_process	negative regulation of apoptotic process
R	0045618	biological_process	positive regulation of keratinocyte differentiation
R	0045682	biological_process	regulation of epidermis development
R	0045893	biological_process	positive regulation of DNA-templated transcription
R	0045944	biological_process	positive regulation of transcription by RNA polymerase II
R	0046042	biological_process	ITP biosynthetic process
R	0046872	molecular_function	metal ion binding
R	0048471	cellular_component	perinuclear region of cytoplasm
R	0050679	biological_process	positive regulation of epithelial cell proliferation
R	0051880	molecular_function	G-quadruplex DNA binding
R	0070062	cellular_component	extracellular exosome
R	0071944	cellular_component	cell periphery
R	0120225	molecular_function	coenzyme A binding
R	1904813	cellular_component	ficolin-1-rich granule lumen
R	2000144	biological_process	positive regulation of DNA-templated transcription initiation
T	0000166	molecular_function	nucleotide binding
T	0001216	molecular_function	DNA-binding transcription activator activity
T	0001726	cellular_component	ruffle
T	0003677	molecular_function	DNA binding
T	0004518	molecular_function	nuclease activity
T	0004550	molecular_function	nucleoside diphosphate kinase activity
T	0004673	molecular_function	protein histidine kinase activity
T	0005515	molecular_function	protein binding
T	0005524	molecular_function	ATP binding
T	0005576	cellular_component	extracellular region
T	0005634	cellular_component	nucleus
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0005925	cellular_component	focal adhesion
T	0006183	biological_process	GTP biosynthetic process
T	0006228	biological_process	UTP biosynthetic process
T	0006241	biological_process	CTP biosynthetic process
T	0007155	biological_process	cell adhesion
T	0007229	biological_process	integrin-mediated signaling pathway
T	0009117	biological_process	nucleotide metabolic process
T	0009142	biological_process	nucleoside triphosphate biosynthetic process
T	0016301	molecular_function	kinase activity
T	0016740	molecular_function	transferase activity
T	0019003	molecular_function	GDP binding
T	0030027	cellular_component	lamellipodium
T	0034774	cellular_component	secretory granule lumen
T	0042304	biological_process	regulation of fatty acid biosynthetic process
T	0042802	molecular_function	identical protein binding
T	0042981	biological_process	regulation of apoptotic process
T	0043066	biological_process	negative regulation of apoptotic process
T	0045618	biological_process	positive regulation of keratinocyte differentiation
T	0045682	biological_process	regulation of epidermis development
T	0045893	biological_process	positive regulation of DNA-templated transcription
T	0045944	biological_process	positive regulation of transcription by RNA polymerase II
T	0046042	biological_process	ITP biosynthetic process
T	0046872	molecular_function	metal ion binding
T	0048471	cellular_component	perinuclear region of cytoplasm
T	0050679	biological_process	positive regulation of epithelial cell proliferation
T	0051880	molecular_function	G-quadruplex DNA binding
T	0070062	cellular_component	extracellular exosome
T	0071944	cellular_component	cell periphery
T	0120225	molecular_function	coenzyme A binding
T	1904813	cellular_component	ficolin-1-rich granule lumen
T	2000144	biological_process	positive regulation of DNA-templated transcription initiation
U	0000166	molecular_function	nucleotide binding
U	0001216	molecular_function	DNA-binding transcription activator activity
U	0001726	cellular_component	ruffle
U	0003677	molecular_function	DNA binding
U	0004518	molecular_function	nuclease activity
U	0004550	molecular_function	nucleoside diphosphate kinase activity
U	0004673	molecular_function	protein histidine kinase activity
U	0005515	molecular_function	protein binding
U	0005524	molecular_function	ATP binding
U	0005576	cellular_component	extracellular region
U	0005634	cellular_component	nucleus
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0005925	cellular_component	focal adhesion
U	0006183	biological_process	GTP biosynthetic process
U	0006228	biological_process	UTP biosynthetic process
U	0006241	biological_process	CTP biosynthetic process
U	0007155	biological_process	cell adhesion
U	0007229	biological_process	integrin-mediated signaling pathway
U	0009117	biological_process	nucleotide metabolic process
U	0009142	biological_process	nucleoside triphosphate biosynthetic process
U	0016301	molecular_function	kinase activity
U	0016740	molecular_function	transferase activity
U	0019003	molecular_function	GDP binding
U	0030027	cellular_component	lamellipodium
U	0034774	cellular_component	secretory granule lumen
U	0042304	biological_process	regulation of fatty acid biosynthetic process
U	0042802	molecular_function	identical protein binding
U	0042981	biological_process	regulation of apoptotic process
U	0043066	biological_process	negative regulation of apoptotic process
U	0045618	biological_process	positive regulation of keratinocyte differentiation
U	0045682	biological_process	regulation of epidermis development
U	0045893	biological_process	positive regulation of DNA-templated transcription
U	0045944	biological_process	positive regulation of transcription by RNA polymerase II
U	0046042	biological_process	ITP biosynthetic process
U	0046872	molecular_function	metal ion binding
U	0048471	cellular_component	perinuclear region of cytoplasm
U	0050679	biological_process	positive regulation of epithelial cell proliferation
U	0051880	molecular_function	G-quadruplex DNA binding
U	0070062	cellular_component	extracellular exosome
U	0071944	cellular_component	cell periphery
U	0120225	molecular_function	coenzyme A binding
U	1904813	cellular_component	ficolin-1-rich granule lumen
U	2000144	biological_process	positive regulation of DNA-templated transcription initiation

Functional Information from PROSITE/UniProt

site_id	PS00469
Number of Residues	9
Details	NDPK Nucleoside diphosphate kinase (NDPK) active site signature. NiiHGSDSV

Chain	Residue	Details
R	ASN115-VAL123

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	390
Details	Region: {"description":"Interaction with AKAP13","evidences":[{"source":"PubMed","id":"15249197","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"description":"Pros-phosphohistidine intermediate","evidences":[{"source":"PubMed","id":"1851158","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19435876","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3BBF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
R	ASN115
R	LYS12

---

site_id	CSA10
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
U	TYR52
U	LYS12

---

site_id	CSA11
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
N	TYR52
N	LYS12

---

site_id	CSA12
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
O	TYR52
O	LYS12

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
L	ASN115
L	LYS12

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
T	ASN115
T	LYS12

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
U	ASN115
U	LYS12

---

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
N	ASN115
N	LYS12

---

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
O	ASN115
O	LYS12

---

site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
R	TYR52
R	LYS12

---

site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
L	TYR52
L	LYS12

---

site_id	CSA9
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsp

Chain	Residue	Details
T	TYR52
T	LYS12

---