Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1NSI

HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE, ZN-BOUND, L-ARG COMPLEX

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004517	molecular_function	nitric-oxide synthase activity
A	0006809	biological_process	nitric oxide biosynthetic process
B	0004517	molecular_function	nitric-oxide synthase activity
B	0006809	biological_process	nitric oxide biosynthetic process
C	0004517	molecular_function	nitric-oxide synthase activity
C	0006809	biological_process	nitric oxide biosynthetic process
D	0004517	molecular_function	nitric-oxide synthase activity
D	0006809	biological_process	nitric oxide biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 910

Chain	Residue
A	ARG301
A	HOH1015
A	HOH1016
A	HOH1017

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 920

Chain	Residue
A	LYS335
A	TYR336
A	GLU337
A	TRP338
D	ARG340

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 930

Chain	Residue
A	ASN236
A	ASN239
C	ASN236
C	ASN239

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 911

Chain	Residue
B	GLY300
B	ARG301
B	HOH973

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 921

Chain	Residue
B	LYS335
B	GLU337
B	TRP338
D	TYR336
D	HOH960

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 C 912

Chain	Residue
C	ARG301

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 922

Chain	Residue
B	ARG340
C	LYS335
C	TYR336
C	GLU337
C	TRP338
C	HOH977

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 913

Chain	Residue
D	ARG301
D	HOH961

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 923

Chain	Residue
D	LYS335
D	TYR336
D	GLU337
D	TRP338
D	HOH925

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 901

Chain	Residue
A	CYS110
A	CYS115
B	CYS110
B	CYS115

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 902

Chain	Residue
C	CYS110
C	CYS115
D	CYS110
D	CYS115

site_id	BC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE HEM A 550

Chain	Residue
A	TRP194
A	ALA197
A	ARG199
A	CYS200
A	SER242
A	PHE369
A	ASN370
A	GLY371
A	TRP372
A	TRP463
A	TYR491
A	H4B600
A	ARG700
A	HOH943
A	HOH953

site_id	BC4
Number of Residues	12
Details	BINDING SITE FOR RESIDUE H4B A 600

Chain	Residue
A	SER118
A	ARG381
A	ILE462
A	TRP463
A	HEM550
A	HOH964
A	HOH999
B	TRP461
B	PHE476
B	HIS477
B	GLN478
B	GLU479

site_id	BC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ARG A 700

Chain	Residue
A	GLN263
A	TYR347
A	PRO350
A	TRP372
A	TYR373
A	GLU377
A	ASP382
A	HEM550

site_id	BC6
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM B 550

Chain	Residue
B	TRP194
B	ARG199
B	CYS200
B	PHE369
B	ASN370
B	TRP372
B	GLU377
B	TRP463
B	TYR489
B	TYR491
B	H4B601
B	ARG701
B	HOH932
B	HOH995

site_id	BC7
Number of Residues	14
Details	BINDING SITE FOR RESIDUE H4B B 601

Chain	Residue
B	TRP463
B	HEM550
B	HOH928
B	HOH932
B	HOH986
B	HOH987
B	HOH997
A	TRP461
A	PHE476
A	HIS477
A	GLN478
B	SER118
B	ARG381
B	ILE462

site_id	BC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ARG B 701

Chain	Residue
B	GLN263
B	TYR347
B	PRO350
B	TRP372
B	TYR373
B	GLU377
B	ASP382
B	HEM550

site_id	BC9
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM C 550

Chain	Residue
C	TRP194
C	ARG199
C	CYS200
C	SER242
C	PHE369
C	ASN370
C	GLY371
C	TRP372
C	GLU377
C	TRP463
C	TYR491
C	H4B602
C	ARG702
C	HOH934

site_id	CC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE H4B C 602

Chain	Residue
C	SER118
C	ARG381
C	ILE462
C	TRP463
C	HEM550
C	HOH931
C	HOH934
C	HOH960
D	TRP461
D	PHE476
D	HIS477
D	GLN478
D	GLU479

site_id	CC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ARG C 702

Chain	Residue
C	GLN263
C	TYR347
C	TRP372
C	TYR373
C	GLU377
C	ASP382
C	HEM550

site_id	CC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE HEM D 550

Chain	Residue
D	TRP194
D	ALA197
D	CYS200
D	PHE369
D	ASN370
D	TRP372
D	GLU377
D	TRP463
D	TYR491
D	H4B603
D	ARG703

site_id	CC4
Number of Residues	12
Details	BINDING SITE FOR RESIDUE H4B D 603

Chain	Residue
C	TRP461
C	PHE476
C	HIS477
C	GLN478
C	GLU479
D	SER118
D	ARG381
D	ILE462
D	TRP463
D	HEM550
D	HOH936
D	HOH959

site_id	CC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE ARG D 703

Chain	Residue
D	GLN263
D	TYR347
D	PRO350
D	TRP372
D	TYR373
D	GLU377
D	ASP382
D	HEM550
D	HOH944

site_id	CC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 880

Chain	Residue
A	ASP256
A	ARG258
A	GLN310
A	THR498
A	HIS499

site_id	CC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 881

Chain	Residue
B	ASP256
B	ARG258
B	GLN310
B	THR498
B	HIS499

site_id	CC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL C 882

Chain	Residue
C	ASP256
C	ARG258
C	GLN310
C	ALA495
C	THR498
C	HIS499

site_id	CC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 883

Chain	Residue
D	ASP256
D	ARG258
D	GLN310
D	THR498
D	HIS499

Functional Information from PROSITE/UniProt

site_id	PS60001
Number of Residues	8
Details	NOS Nitric oxide synthase (NOS) signature. RCIGRIqW

Chain	Residue	Details
A	ARG199-TRP206

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10074942","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10409685","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4NOS","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10074942","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10409685","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4NOS","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"description":"axial binding residue","evidences":[{"source":"PubMed","id":"10074942","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10409685","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4NOS","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P29474","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine; by PKA","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 3nos

Chain	Residue	Details
A	TRP372
A	CYS200
A	GLU377
A	ARG203

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 3nos

Chain	Residue	Details
B	TRP372
B	CYS200
B	GLU377
B	ARG203

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 3nos

Chain	Residue	Details
C	TRP372
C	CYS200
C	GLU377
C	ARG203

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 3nos

Chain	Residue	Details
D	TRP372
D	CYS200
D	GLU377
D	ARG203

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)