1N8W
Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0001968 | molecular_function | fibronectin binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004474 | molecular_function | malate synthase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005518 | molecular_function | collagen binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006097 | biological_process | glyoxylate cycle |
| A | 0006099 | biological_process | tricarboxylic acid cycle |
| A | 0009274 | cellular_component | peptidoglycan-based cell wall |
| A | 0009436 | biological_process | glyoxylate catabolic process |
| A | 0009986 | cellular_component | cell surface |
| A | 0015936 | biological_process | coenzyme A metabolic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0042603 | cellular_component | capsule |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0043236 | molecular_function | laminin binding |
| A | 0044406 | biological_process | adhesion of symbiont to host |
| A | 0046810 | molecular_function | host cell extracellular matrix binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0120225 | molecular_function | coenzyme A binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0001968 | molecular_function | fibronectin binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004474 | molecular_function | malate synthase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005518 | molecular_function | collagen binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006097 | biological_process | glyoxylate cycle |
| B | 0006099 | biological_process | tricarboxylic acid cycle |
| B | 0009274 | cellular_component | peptidoglycan-based cell wall |
| B | 0009436 | biological_process | glyoxylate catabolic process |
| B | 0009986 | cellular_component | cell surface |
| B | 0015936 | biological_process | coenzyme A metabolic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0042603 | cellular_component | capsule |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0043236 | molecular_function | laminin binding |
| B | 0044406 | biological_process | adhesion of symbiont to host |
| B | 0046810 | molecular_function | host cell extracellular matrix binding |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0120225 | molecular_function | coenzyme A binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 900 |
| Chain | Residue |
| A | GLU434 |
| A | ASP462 |
| A | MLT901 |
| A | HOH1001 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 1900 |
| Chain | Residue |
| B | GLU434 |
| B | ASP462 |
| B | HOH1139 |
| B | HOH1140 |
| B | GLV1901 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1800 |
| Chain | Residue |
| A | ARG534 |
| A | GLN557 |
| A | HOH1179 |
| B | ARG69 |
| B | ARG70 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1802 |
| Chain | Residue |
| A | SER247 |
| A | GLN248 |
| A | LYS258 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1803 |
| Chain | Residue |
| A | ARG14 |
| site_id | AC6 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE MLT A 901 |
| Chain | Residue |
| A | ASP274 |
| A | ARG339 |
| A | GLU434 |
| A | GLY459 |
| A | PHE460 |
| A | LEU461 |
| A | ASP462 |
| A | TRP541 |
| A | ASP633 |
| A | ALA635 |
| A | MG900 |
| A | COA903 |
| A | HOH1001 |
| A | HOH1045 |
| site_id | AC7 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE MLT A 902 |
| Chain | Residue |
| A | LEU122 |
| A | ASN123 |
| A | SER275 |
| A | VAL276 |
| A | ALA277 |
| A | GLY618 |
| A | CYS619 |
| A | HOH1144 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MLT B 1902 |
| Chain | Residue |
| B | LEU122 |
| B | ASN123 |
| B | SER275 |
| B | VAL617 |
| B | GLY618 |
| B | CYS619 |
| B | HOH1039 |
| site_id | AC9 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE COA A 903 |
| Chain | Residue |
| A | VAL118 |
| A | VAL119 |
| A | ARG125 |
| A | PHE126 |
| A | ASN129 |
| A | SER275 |
| A | ARG312 |
| A | ARG339 |
| A | MET515 |
| A | PRO543 |
| A | PRO545 |
| A | CYS619 |
| A | SER620 |
| A | LYS621 |
| A | MET631 |
| A | ASP633 |
| A | MLT901 |
| A | HOH1343 |
| A | HOH1357 |
| site_id | BC1 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE GLV B 1901 |
| Chain | Residue |
| B | ARG339 |
| B | MET432 |
| B | GLU434 |
| B | GLY459 |
| B | PHE460 |
| B | LEU461 |
| B | ASP462 |
| B | TRP541 |
| B | HOH1139 |
| B | HOH1140 |
| B | HOH1432 |
| B | MG1900 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| A | ARG339 | |
| B | ARG339 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor |
| Chain | Residue | Details |
| A | ASP633 | |
| B | ASP633 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 24 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | VAL118 | |
| A | PRO543 | |
| A | GLY618 | |
| A | ASP633 | |
| B | VAL118 | |
| B | ARG125 | |
| B | SER275 | |
| B | LYS305 | |
| B | ARG312 | |
| B | ARG339 | |
| B | GLU434 | |
| A | ARG125 | |
| B | GLY459 | |
| B | ASP462 | |
| B | PRO543 | |
| B | GLY618 | |
| B | ASP633 | |
| A | SER275 | |
| A | LYS305 | |
| A | ARG312 | |
| A | ARG339 | |
| A | GLU434 | |
| A | GLY459 | |
| A | ASP462 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: Cysteine sulfenic acid (-SOH) => ECO:0000255|HAMAP-Rule:MF_00641 |
| Chain | Residue | Details |
| A | CYS619 | |
| B | CYS619 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1d8c |
| Chain | Residue | Details |
| A | GLU273 | |
| A | ARG339 | |
| A | ASP633 | |
| A | ASP271 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1d8c |
| Chain | Residue | Details |
| B | GLU273 | |
| B | ARG339 | |
| B | ASP633 | |
| B | ASP271 |
