1N8F
Crystal structure of E24Q mutant of phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHP synthase) from Escherichia Coli in complex with Mn2+ and PEP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009058 | biological_process | biosynthetic process |
| A | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| A | 0009423 | biological_process | chorismate biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0042802 | molecular_function | identical protein binding |
| B | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009058 | biological_process | biosynthetic process |
| B | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| B | 0009423 | biological_process | chorismate biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0042802 | molecular_function | identical protein binding |
| C | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0009058 | biological_process | biosynthetic process |
| C | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| C | 0009423 | biological_process | chorismate biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0042802 | molecular_function | identical protein binding |
| D | 0003849 | molecular_function | 3-deoxy-7-phosphoheptulonate synthase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0009058 | biological_process | biosynthetic process |
| D | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| D | 0009423 | biological_process | chorismate biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0042802 | molecular_function | identical protein binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MN A 351 |
| Chain | Residue |
| A | CYS61 |
| A | HIS268 |
| A | GLU302 |
| A | ASP326 |
| A | PEP1396 |
| A | HOH5416 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MN B 351 |
| Chain | Residue |
| B | ASP326 |
| B | PEP1397 |
| B | HOH2376 |
| B | CYS61 |
| B | HIS268 |
| B | GLU302 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MN C 351 |
| Chain | Residue |
| C | CYS61 |
| C | HIS268 |
| C | GLU302 |
| C | ASP326 |
| C | PEP1398 |
| C | HOH3451 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MN D 351 |
| Chain | Residue |
| D | CYS61 |
| D | HIS268 |
| D | GLU302 |
| D | ASP326 |
| D | PEP1399 |
| D | HOH4414 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 5353 |
| Chain | Residue |
| A | ARG99 |
| A | THR100 |
| A | HOH5446 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 2353 |
| Chain | Residue |
| B | ARG99 |
| B | THR100 |
| B | HOH2404 |
| B | HOH2433 |
| B | HOH2557 |
| B | HOH2672 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3353 |
| Chain | Residue |
| C | ARG99 |
| C | THR100 |
| C | HOH3480 |
| C | HOH3509 |
| C | HOH3552 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 D 4353 |
| Chain | Residue |
| D | ARG99 |
| D | THR100 |
| D | HOH4444 |
| D | HOH4474 |
| D | HOH4547 |
| D | HOH4628 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A 5356 |
| Chain | Residue |
| A | THR192 |
| A | ILE193 |
| A | LYS194 |
| A | HOH5370 |
| A | HOH5553 |
| A | HOH5587 |
| A | HOH5654 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 2356 |
| Chain | Residue |
| B | THR192 |
| B | ILE193 |
| B | LYS194 |
| B | HOH2467 |
| B | HOH2591 |
| B | HOH2641 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3356 |
| Chain | Residue |
| C | THR192 |
| C | ILE193 |
| C | LYS194 |
| C | HOH3405 |
| C | HOH3544 |
| C | HOH3575 |
| site_id | BC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 4356 |
| Chain | Residue |
| D | THR192 |
| D | ILE193 |
| D | LYS194 |
| site_id | BC4 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE PEP A 1396 |
| Chain | Residue |
| A | ARG92 |
| A | LYS97 |
| A | PRO98 |
| A | GLU143 |
| A | GLY163 |
| A | ALA164 |
| A | ARG165 |
| A | LYS186 |
| A | ARG234 |
| A | HIS268 |
| A | MN351 |
| A | HOH5406 |
| A | HOH5413 |
| A | HOH5416 |
| A | HOH5432 |
| A | HOH5461 |
| site_id | BC5 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE PEP B 1397 |
| Chain | Residue |
| B | ARG92 |
| B | LYS97 |
| B | GLU143 |
| B | GLY163 |
| B | ALA164 |
| B | ARG165 |
| B | LYS186 |
| B | ARG234 |
| B | HIS268 |
| B | MN351 |
| B | HOH2366 |
| B | HOH2373 |
| B | HOH2376 |
| B | HOH2391 |
| B | HOH2419 |
| site_id | BC6 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE PEP C 1398 |
| Chain | Residue |
| C | GLU143 |
| C | GLY163 |
| C | ALA164 |
| C | ARG165 |
| C | LYS186 |
| C | ARG234 |
| C | HIS268 |
| C | GLU302 |
| C | MN351 |
| C | HOH3441 |
| C | HOH3448 |
| C | HOH3451 |
| C | HOH3467 |
| C | HOH3495 |
| C | ARG92 |
| C | LYS97 |
| site_id | BC7 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE PEP D 1399 |
| Chain | Residue |
| D | ARG92 |
| D | LYS97 |
| D | GLU143 |
| D | GLY163 |
| D | ALA164 |
| D | ARG165 |
| D | LYS186 |
| D | ARG234 |
| D | HIS268 |
| D | MN351 |
| D | HOH4404 |
| D | HOH4411 |
| D | HOH4414 |
| D | HOH4431 |
| D | HOH4460 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| A | HIS268 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| B | HIS268 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| C | HIS268 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1q3n |
| Chain | Residue | Details |
| D | HIS268 |
