1N2Z

2.0 Angstrom structure of BtuF, the vitamin B12 binding protein of E. coli

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0015889	biological_process	cobalamin transport
A	0031419	molecular_function	cobalamin binding
A	0042597	cellular_component	periplasmic space
B	0015889	biological_process	cobalamin transport
B	0031419	molecular_function	cobalamin binding
B	0042597	cellular_component	periplasmic space

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CD A 402

Chain	Residue
A	GLU245
A	CL426
A	CL427
A	CL429
A	HOH443
A	HOH459

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD A 403

Chain	Residue
A	HOH431
A	HOH442
A	ASP52
A	ASP242
A	ARG246

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CD A 404

Chain	Residue
A	THR215
A	CL428
A	CL429
A	HOH443
A	HOH459
A	HOH515

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD A 405

Chain	Residue
A	ASP266
A	HOH445
B	GLU181
B	GLU186
B	LYS190

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CD B 406

Chain	Residue
B	THR215
B	GLU245
B	CL424
B	HOH430
B	HOH438
B	HOH481

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD B 407

Chain	Residue
B	ASP52
B	ASP242
B	ARG246
B	CL425
B	HOH426

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD B 408

Chain	Residue
B	THR215
B	HOH438
B	HOH468
B	HOH481

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD B 409

Chain	Residue
A	HOH434
B	GLU173
B	HOH519
B	HOH587

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD B 410

Chain	Residue
A	TRP243
A	ARG250
B	GLN149
B	GLN256
B	HOH432

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD A 411

Chain	Residue
A	ARG86
B	ASP108
B	ALA109
B	HOH429
B	HOH482

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A 412

Chain	Residue
A	ASP108
A	ALA109
A	HOH436
B	ARG86

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A 413

Chain	Residue
A	ASP266
A	HOH445
A	HOH475
A	HOH562

---

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CD A 414

Chain	Residue
A	GLU181
A	LYS190
B	ARG92

---

site_id	BC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CD A 415

Chain	Residue
A	ASP130
A	HOH504

---

site_id	BC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CD B 416

Chain	Residue
B	ASP219
B	CD422

---

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A 417

Chain	Residue
A	ASP219
A	HOH484
A	HOH528
B	ASP266

---

site_id	BC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CD A 418

Chain	Residue
A	HOH499
B	HOH507

---

site_id	BC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CD A 419

Chain	Residue
A	GLU91
A	HOH603

---

site_id	CC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CD B 420

Chain	Residue
B	HOH529
B	HOH574
B	HOH597

---

site_id	CC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CD A 421

Chain	Residue
A	HOH513

---

site_id	CC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CD B 422

Chain	Residue
B	ASP191
B	ARG207
B	CD416

---

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD B 423

Chain	Residue
A	GLU173
A	HOH537
B	ARG86
B	GLU91
B	HOH528

---

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL B 424

Chain	Residue
B	GLU245
B	CNC401
B	CD406

---

site_id	CC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL B 425

Chain	Residue
B	TYR50
B	ARG246
B	CD407

---

site_id	CC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL A 426

Chain	Residue
A	GLU245
A	CNC400
A	CD402
A	CL427
A	HOH621

---

site_id	CC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL A 427

Chain	Residue
A	CL426
A	CL429
A	CNC400
A	CD402

---

site_id	CC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL A 428

Chain	Residue
A	GLN161
A	GLY163
A	ILE214
A	THR215
A	CD404

---

site_id	DC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL A 429

Chain	Residue
A	CD402
A	CD404
A	CL427
A	HOH459

---

site_id	DC2
Number of Residues	27
Details	BINDING SITE FOR RESIDUE CNC A 400

Chain	Residue
A	PRO31
A	ALA32
A	TYR50
A	TRP66
A	TRP85
A	GLY87
A	GLY88
A	PHE168
A	TRP196
A	ASP242
A	GLU245
A	ARG246
A	CL426
A	CL427
A	HOH433
A	HOH455
A	HOH490
A	HOH496
A	HOH503
A	HOH512
A	HOH614
A	HOH620
A	HOH622
A	HOH623
A	HOH624
A	HOH625
A	HOH627

---

site_id	DC3
Number of Residues	23
Details	BINDING SITE FOR RESIDUE CNC B 401

Chain	Residue
B	PRO31
B	ALA32
B	TYR50
B	TRP66
B	TRP85
B	GLY87
B	GLY88
B	PHE168
B	TRP196
B	SER241
B	ASP242
B	GLU245
B	ARG246
B	CL424
B	HOH431
B	HOH455
B	HOH459
B	HOH472
B	HOH526
B	HOH530
B	HOH599
B	HOH600
B	HOH601

---

site_id	DC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PG4 A 430

Chain	Residue
A	PRO124
A	TRP125
B	PRO124
B	TRP125

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	482
Details	Domain: {"description":"Fe/B12 periplasmic-binding","evidences":[{"source":"HAMAP-Rule","id":"MF_01000","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01000","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12468528","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1N4A","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01000","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12468528","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12475936","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1N2Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1N4A","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Site: {"description":"Important for BtuC binding","evidences":[{"source":"HAMAP-Rule","id":"MF_01000","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12475936","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---