Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1ML5

Structure of the E. coli ribosomal termination complex with release factor 2

Functional Information from GO Data

Chain	GOid	namespace	contents
c	0003723	molecular_function	RNA binding
c	0003735	molecular_function	structural constituent of ribosome
c	0006412	biological_process	translation
c	0015934	cellular_component	large ribosomal subunit
d	0003723	molecular_function	RNA binding
d	0003735	molecular_function	structural constituent of ribosome
d	0005840	cellular_component	ribosome
d	0006412	biological_process	translation
d	0015934	cellular_component	large ribosomal subunit
d	0016740	molecular_function	transferase activity
e	0003735	molecular_function	structural constituent of ribosome
e	0005840	cellular_component	ribosome
e	0006412	biological_process	translation
E	0003735	molecular_function	structural constituent of ribosome
E	0005840	cellular_component	ribosome
E	0006412	biological_process	translation
E	0015935	cellular_component	small ribosomal subunit
E	0022627	cellular_component	cytosolic small ribosomal subunit
f	0003735	molecular_function	structural constituent of ribosome
f	0005840	cellular_component	ribosome
f	0006412	biological_process	translation
f	0019843	molecular_function	rRNA binding
F	0003676	molecular_function	nucleic acid binding
F	0003723	molecular_function	RNA binding
F	0003729	molecular_function	mRNA binding
F	0003735	molecular_function	structural constituent of ribosome
F	0006412	biological_process	translation
F	0019843	molecular_function	rRNA binding
F	0022627	cellular_component	cytosolic small ribosomal subunit
g	0003735	molecular_function	structural constituent of ribosome
g	0005840	cellular_component	ribosome
g	0006412	biological_process	translation
G	0003723	molecular_function	RNA binding
G	0003735	molecular_function	structural constituent of ribosome
G	0006412	biological_process	translation
G	0015935	cellular_component	small ribosomal subunit
G	0019843	molecular_function	rRNA binding
G	0042274	biological_process	ribosomal small subunit biogenesis
h	0000027	biological_process	ribosomal large subunit assembly
h	0002181	biological_process	cytoplasmic translation
h	0003723	molecular_function	RNA binding
h	0003735	molecular_function	structural constituent of ribosome
h	0005737	cellular_component	cytoplasm
h	0005829	cellular_component	cytosol
h	0005840	cellular_component	ribosome
h	0006412	biological_process	translation
h	0019843	molecular_function	rRNA binding
h	0022625	cellular_component	cytosolic large ribosomal subunit
h	0070180	molecular_function	large ribosomal subunit rRNA binding
H	0003723	molecular_function	RNA binding
H	0003735	molecular_function	structural constituent of ribosome
H	0005737	cellular_component	cytoplasm
H	0005840	cellular_component	ribosome
H	0006412	biological_process	translation
H	0015935	cellular_component	small ribosomal subunit
H	0019843	molecular_function	rRNA binding
I	0003735	molecular_function	structural constituent of ribosome
I	0005737	cellular_component	cytoplasm
I	0005840	cellular_component	ribosome
I	0006412	biological_process	translation
I	0019843	molecular_function	rRNA binding
I	0070181	molecular_function	small ribosomal subunit rRNA binding
J	0003723	molecular_function	RNA binding
J	0003735	molecular_function	structural constituent of ribosome
J	0006412	biological_process	translation
J	0015935	cellular_component	small ribosomal subunit
K	0003735	molecular_function	structural constituent of ribosome
K	0005737	cellular_component	cytoplasm
K	0005840	cellular_component	ribosome
K	0006412	biological_process	translation
K	0019843	molecular_function	rRNA binding
l	0000027	biological_process	ribosomal large subunit assembly
l	0002181	biological_process	cytoplasmic translation
l	0003735	molecular_function	structural constituent of ribosome
l	0005515	molecular_function	protein binding
l	0005737	cellular_component	cytoplasm
l	0005829	cellular_component	cytosol
l	0005840	cellular_component	ribosome
l	0006412	biological_process	translation
l	0006415	biological_process	translational termination
l	0015968	biological_process	stringent response
l	0019843	molecular_function	rRNA binding
l	0022625	cellular_component	cytosolic large ribosomal subunit
l	0070180	molecular_function	large ribosomal subunit rRNA binding
L	0003723	molecular_function	RNA binding
L	0003735	molecular_function	structural constituent of ribosome
L	0005737	cellular_component	cytoplasm
L	0005840	cellular_component	ribosome
L	0006412	biological_process	translation
L	0015935	cellular_component	small ribosomal subunit
L	0022627	cellular_component	cytosolic small ribosomal subunit
m	0003735	molecular_function	structural constituent of ribosome
m	0005840	cellular_component	ribosome
m	0006412	biological_process	translation
m	0015934	cellular_component	large ribosomal subunit
M	0000049	molecular_function	tRNA binding
M	0003723	molecular_function	RNA binding
M	0003735	molecular_function	structural constituent of ribosome
M	0005840	cellular_component	ribosome
M	0006412	biological_process	translation
n	0002181	biological_process	cytoplasmic translation
n	0003735	molecular_function	structural constituent of ribosome
n	0005515	molecular_function	protein binding
n	0005737	cellular_component	cytoplasm
n	0005829	cellular_component	cytosol
n	0005840	cellular_component	ribosome
n	0006412	biological_process	translation
n	0015934	cellular_component	large ribosomal subunit
n	0019843	molecular_function	rRNA binding
n	0022625	cellular_component	cytosolic large ribosomal subunit
n	0070180	molecular_function	large ribosomal subunit rRNA binding
N	0003735	molecular_function	structural constituent of ribosome
N	0005840	cellular_component	ribosome
N	0006412	biological_process	translation
N	0019843	molecular_function	rRNA binding
o	0003735	molecular_function	structural constituent of ribosome
o	0005840	cellular_component	ribosome
o	0006412	biological_process	translation
o	0015934	cellular_component	large ribosomal subunit
O	0000049	molecular_function	tRNA binding
O	0003735	molecular_function	structural constituent of ribosome
O	0005840	cellular_component	ribosome
O	0006412	biological_process	translation
O	0015935	cellular_component	small ribosomal subunit
O	0019843	molecular_function	rRNA binding
p	0003735	molecular_function	structural constituent of ribosome
p	0005840	cellular_component	ribosome
p	0006412	biological_process	translation
P	0000049	molecular_function	tRNA binding
P	0003676	molecular_function	nucleic acid binding
P	0003723	molecular_function	RNA binding
P	0003735	molecular_function	structural constituent of ribosome
P	0005829	cellular_component	cytosol
P	0005840	cellular_component	ribosome
P	0006412	biological_process	translation
P	0015935	cellular_component	small ribosomal subunit
P	0019843	molecular_function	rRNA binding
q	0003735	molecular_function	structural constituent of ribosome
q	0005840	cellular_component	ribosome
q	0006412	biological_process	translation
q	0008097	molecular_function	5S rRNA binding
Q	0003735	molecular_function	structural constituent of ribosome
Q	0005840	cellular_component	ribosome
Q	0006412	biological_process	translation
Q	0008270	molecular_function	zinc ion binding
Q	0015935	cellular_component	small ribosomal subunit
Q	0019843	molecular_function	rRNA binding
r	0003735	molecular_function	structural constituent of ribosome
r	0006412	biological_process	translation
r	0008270	molecular_function	zinc ion binding
r	0019843	molecular_function	rRNA binding
R	0003735	molecular_function	structural constituent of ribosome
R	0005737	cellular_component	cytoplasm
R	0005840	cellular_component	ribosome
R	0006412	biological_process	translation
R	0019843	molecular_function	rRNA binding
R	0022627	cellular_component	cytosolic small ribosomal subunit
s	0003735	molecular_function	structural constituent of ribosome
s	0005840	cellular_component	ribosome
s	0006412	biological_process	translation
s	0015934	cellular_component	large ribosomal subunit
s	0019843	molecular_function	rRNA binding
s	0022625	cellular_component	cytosolic large ribosomal subunit
S	0003735	molecular_function	structural constituent of ribosome
S	0005840	cellular_component	ribosome
S	0006412	biological_process	translation
t	0003735	molecular_function	structural constituent of ribosome
t	0005840	cellular_component	ribosome
t	0006412	biological_process	translation
t	0019843	molecular_function	rRNA binding
T	0003735	molecular_function	structural constituent of ribosome
T	0005840	cellular_component	ribosome
T	0006412	biological_process	translation
T	0019843	molecular_function	rRNA binding
T	0022627	cellular_component	cytosolic small ribosomal subunit
u	0003723	molecular_function	RNA binding
u	0003735	molecular_function	structural constituent of ribosome
u	0005840	cellular_component	ribosome
u	0006412	biological_process	translation
u	0015934	cellular_component	large ribosomal subunit
U	0003735	molecular_function	structural constituent of ribosome
U	0005840	cellular_component	ribosome
U	0006412	biological_process	translation
U	0019843	molecular_function	rRNA binding
U	0070181	molecular_function	small ribosomal subunit rRNA binding
v	0000027	biological_process	ribosomal large subunit assembly
v	0002181	biological_process	cytoplasmic translation
v	0003723	molecular_function	RNA binding
v	0003735	molecular_function	structural constituent of ribosome
v	0005737	cellular_component	cytoplasm
v	0005829	cellular_component	cytosol
v	0005840	cellular_component	ribosome
v	0006412	biological_process	translation
v	0008097	molecular_function	5S rRNA binding
v	0009314	biological_process	response to radiation
v	0017148	biological_process	negative regulation of translation
v	0022625	cellular_component	cytosolic large ribosomal subunit
V	0000028	biological_process	ribosomal small subunit assembly
V	0003723	molecular_function	RNA binding
V	0003735	molecular_function	structural constituent of ribosome
V	0005737	cellular_component	cytoplasm
V	0005840	cellular_component	ribosome
V	0006412	biological_process	translation
V	0015935	cellular_component	small ribosomal subunit
V	0019843	molecular_function	rRNA binding
w	0003735	molecular_function	structural constituent of ribosome
w	0005840	cellular_component	ribosome
w	0006412	biological_process	translation
W	0003723	molecular_function	RNA binding
W	0003735	molecular_function	structural constituent of ribosome
W	0005840	cellular_component	ribosome
W	0006412	biological_process	translation
W	0015935	cellular_component	small ribosomal subunit
W	0019843	molecular_function	rRNA binding
W	0070181	molecular_function	small ribosomal subunit rRNA binding
x	0000027	biological_process	ribosomal large subunit assembly
x	0002181	biological_process	cytoplasmic translation
x	0003735	molecular_function	structural constituent of ribosome
x	0005737	cellular_component	cytoplasm
x	0006412	biological_process	translation
x	0015934	cellular_component	large ribosomal subunit
x	0022625	cellular_component	cytosolic large ribosomal subunit
X	0005840	cellular_component	ribosome
Z	0003747	molecular_function	translation release factor activity
Z	0005737	cellular_component	cytoplasm
Z	0005829	cellular_component	cytosol
Z	0006415	biological_process	translational termination
Z	0016149	molecular_function	translation release factor activity, codon specific

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DGNTDGEVDlpDV

Chain	Residue	Details
f	ASP9-VAL21

site_id	PS00049
Number of Residues	27
Details	RIBOSOMAL_L14 Ribosomal protein L14 signature. AVVVrtkrgvrrp.DGsyirFdeNacVI

Chain	Residue	Details
n	ALA60-ILE86

site_id	PS00050
Number of Residues	16
Details	RIBOSOMAL_L23 Ribosomal protein L23 signature. KKAVVRLSedddaqeV

Chain	Residue	Details
t	LYS62-VAL77

site_id	PS00052
Number of Residues	27
Details	RIBOSOMAL_S7 Ribosomal protein S7 signature. DvLvtaFInkimrdGKKnlaarIFydA

Chain	Residue	Details
J	ASP20-ALA46

site_id	PS00053
Number of Residues	18
Details	RIBOSOMAL_S8 Ribosomal protein S8 signature. GiaILSTSkGVLtdreAR

Chain	Residue	Details
K	GLY108-ARG125

site_id	PS00054
Number of Residues	23
Details	RIBOSOMAL_S11 Ribosomal protein S11 signature. IrALqaS.GLqVksivDdTPvPHN

Chain	Residue	Details
N	ILE95-ASN117

site_id	PS00055
Number of Residues	8
Details	RIBOSOMAL_S12 Ribosomal protein S12 signature. KkPNSAlR

Chain	Residue	Details
O	LYS46-ARG53

site_id	PS00056
Number of Residues	13
Details	RIBOSOMAL_S17 Ribosomal protein S17 signature. GDvVeIiEsRPIS

Chain	Residue	Details
T	GLY54-SER66

site_id	PS00057
Number of Residues	24
Details	RIBOSOMAL_S18 Ribosomal protein S18 signature. RDYrn.VevLkrFLSEt.GKIlprRR

Chain	Residue	Details
U	ARG32-ARG55

site_id	PS00063
Number of Residues	16
Details	ALDOKETO_REDUCTASE_3 Aldo/keto reductase family putative active site signature. LAKTIKraRIlGLlPF

Chain	Residue	Details
U	LEU66-PHE81

site_id	PS00323
Number of Residues	25
Details	RIBOSOMAL_S19 Ribosomal protein S19 signature. NGKqhvpvyItenmVGhkLGEFapT

Chain	Residue	Details
V	ASN53-THR77

site_id	PS00358
Number of Residues	17
Details	RIBOSOMAL_L5 Ribosomal protein L5 signature. LgeITGQmpVrTkAKrT

Chain	Residue	Details
g	LEU41-THR57

site_id	PS00359
Number of Residues	16
Details	RIBOSOMAL_L11 Ribosomal protein L11 signature. KiIeGTakSMGiEVvD

Chain	Residue	Details
l	LYS126-ASP141

site_id	PS00360
Number of Residues	19
Details	RIBOSOMAL_S9 Ribosomal protein S9 signature. GGGksGQidAiklGiARAL

Chain	Residue	Details
L	GLY67-LEU85

site_id	PS00361
Number of Residues	16
Details	RIBOSOMAL_S10 Ribosomal protein S10 signature. ArrsGAqvsGPIpLPT

Chain	Residue	Details
M	ALA27-THR42

site_id	PS00362
Number of Residues	31
Details	RIBOSOMAL_S15 Ribosomal protein S15 signature. LseHLkvhKkDhhShrgLlmMvgqrrrLlrY

Chain	Residue	Details
R	LEU39-TYR69

site_id	PS00430
Number of Residues	28
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. srvgkkpieipagvtvtvng...............................................................................................NTVTVKGP

Chain	Residue	Details
h	SER1-PRO28
c	PRO1-LYS46

site_id	PS00464
Number of Residues	25
Details	RIBOSOMAL_L22 Ribosomal protein L22 signature. KrvLpRArGRadiikkrtSHITViL

Chain	Residue	Details
s	LYS83-LEU107

site_id	PS00467
Number of Residues	12
Details	RIBOSOMAL_L2 Ribosomal protein L2 signature. PnvRGVAmNAvD

Chain	Residue	Details
d	PRO222-ASP233

site_id	PS00474
Number of Residues	24
Details	RIBOSOMAL_L3 Ribosomal protein L3 signature. FrageyaDvaGvTkGKGtqGpvkR

Chain	Residue	Details
e	PHE193-ARG216

site_id	PS00475
Number of Residues	31
Details	RIBOSOMAL_L15 Ribosomal protein L15 signature. KVLGaGqVrhe..LtLiaddFSegArekVegaG

Chain	Residue	Details
o	LYS107-GLY137

site_id	PS00525
Number of Residues	9
Details	RIBOSOMAL_L6_1 Ribosomal protein L6 signature 1. PEpYKGKGI

Chain	Residue	Details
h	PRO153-ILE161

site_id	PS00527
Number of Residues	23
Details	RIBOSOMAL_S14 Ribosomal protein S14 signature. R.CvrcgrarsvyrfFGLCRiCLR

Chain	Residue	Details
Q	ARG23-ARG45

site_id	PS00548
Number of Residues	35
Details	RIBOSOMAL_S3 Ribosomal protein S3 signature. ARtewaaqGrVplHt..LranIDygfalarTtyGvlG

Chain	Residue	Details
F	ALA163-GLY197

site_id	PS00579
Number of Residues	15
Details	RIBOSOMAL_L29 Ribosomal protein L29 signature. NPGRIKeLRKAIARI

Chain	Residue	Details
w	ASN42-ILE56

site_id	PS00585
Number of Residues	33
Details	RIBOSOMAL_S5 Ribosomal protein S5 signature. GRrfrFgAlvVVGDrq.GrVGlGfgkap.EVpl.AV

Chain	Residue	Details
H	GLY23-VAL55

site_id	PS00632
Number of Residues	25
Details	RIBOSOMAL_S4 Ribosomal protein S4 signature. LEsRLdnvVYRlgfAvSrrqARqLV

Chain	Residue	Details
G	LEU97-VAL121

site_id	PS00634
Number of Residues	33
Details	RIBOSOMAL_L30 Ribosomal protein L30 signature. ALkaLgLrRlqQeRvledtpairgNVekVahLV

Chain	Residue	Details
x	ALA22-VAL54

site_id	PS00646
Number of Residues	14
Details	RIBOSOMAL_S13_1 Ribosomal protein S13 signature. RGlRHrrGlpVRGQ

Chain	Residue	Details
P	ARG88-GLN101

site_id	PS00745
Number of Residues	17
Details	RF_PROK_I Prokaryotic-type class I peptide chain release factors signature. RTsGaGGQHVNrteSAV

Chain	Residue	Details
Z	ARG245-VAL261

site_id	PS00783
Number of Residues	24
Details	RIBOSOMAL_L13 Ribosomal protein L13 signature. IRGMLPhkkqrGReafesVrVYlG

Chain	Residue	Details
m	ILE83-GLY106

site_id	PS00939
Number of Residues	27
Details	RIBOSOMAL_L1E Ribosomal protein L1e signature. NdkeRqlAVrSALaATAdadlvadRGH

Chain	Residue	Details
f	ASN103-HIS129

site_id	PS00962
Number of Residues	12
Details	RIBOSOMAL_S2_1 Ribosomal protein S2 signature 1. VkELLEAGVHFG

Chain	Residue	Details
E	VAL7-GLY18

site_id	PS01048
Number of Residues	10
Details	RIBOSOMAL_S6 Ribosomal protein S6 signature. GlRRLAYpIA

Chain	Residue	Details
I	GLY44-ALA53

site_id	PS01073
Number of Residues	18
Details	RIBOSOMAL_L24E Ribosomal protein L24e signature. YcGtdIePGtGtmFvHkD

Chain	Residue	Details
r	TYR8-ASP25

site_id	PS01108
Number of Residues	18
Details	RIBOSOMAL_L24 Ribosomal protein L24 signature. GDtVeVLrGdfAGee.GeV

Chain	Residue	Details
u	GLY45-VAL62

site_id	PS01199
Number of Residues	20
Details	RIBOSOMAL_L1 Ribosomal protein L1 signature. MgaVgskLGriLGPRGlLPN

Chain	Residue	Details
c	MET120-ASN139

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Motif: {"description":"GGQ motif"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	1
Details	Modified residue: {"description":"N5-methylglutamine","evidences":[{"source":"PubMed","id":"11118225","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11847124","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28077875","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	45
Details	Coiled coil: {}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	68
Details	Domain: {"description":"KH type-2"}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	62
Details	Domain: {"description":"S4 RNA-binding"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11866529","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	63
Details	Domain: {"description":"S5 DRBM","evidences":[{"source":"HAMAP-Rule","id":"MF_01307","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	51
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	21
Details	Compositional bias: {"description":"Basic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"3-methylthioaspartic acid","evidences":[{"source":"PubMed","id":"16287167","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25775268","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	6
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"PubMed","id":"21151122","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	1
Details	Modified residue: {"description":"N5-methylglutamine","evidences":[{"source":"PubMed","id":"365579","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"331083","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	11
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"HAMAP-Rule","id":"MF_01365","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	1
Details	Modified residue: {"description":"N6,N6,N6-trimethyllysine","evidences":[{"source":"PubMed","id":"7004866","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	30
Details	Zinc finger: {"description":"C4-type","evidences":[{"source":"HAMAP-Rule","id":"MF_00773","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00773","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)