1MKB

ESCHERICHIA COLI BETA-HYDROXYDECANOYL THIOL ESTER DEHYDRASE AT PH 5 AND 21 DEGREES C

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004317	molecular_function	(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006633	biological_process	fatty acid biosynthetic process
A	0006636	biological_process	unsaturated fatty acid biosynthetic process
A	0008659	molecular_function	(3R)-3-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity
A	0008693	molecular_function	(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity
A	0016829	molecular_function	lyase activity
A	0016853	molecular_function	isomerase activity
A	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
A	0034017	molecular_function	trans-2-decenoyl-acyl-carrier-protein isomerase activity
A	0042803	molecular_function	protein homodimerization activity
A	0047450	molecular_function	(3R)-3-hydroxybutanoyl-[acyl-carrier-protein] hydratase activity
A	0047451	molecular_function	(3R)-3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
B	0003824	molecular_function	catalytic activity
B	0004317	molecular_function	(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006633	biological_process	fatty acid biosynthetic process
B	0006636	biological_process	unsaturated fatty acid biosynthetic process
B	0008659	molecular_function	(3R)-3-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity
B	0008693	molecular_function	(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity
B	0016829	molecular_function	lyase activity
B	0016853	molecular_function	isomerase activity
B	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
B	0034017	molecular_function	trans-2-decenoyl-acyl-carrier-protein isomerase activity
B	0042803	molecular_function	protein homodimerization activity
B	0047450	molecular_function	(3R)-3-hydroxybutanoyl-[acyl-carrier-protein] hydratase activity
B	0047451	molecular_function	(3R)-3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	TWO POLAR RESIDUES ARE FOUND IN THE CATALYTIC REGION OF THE ACTIVE SITE.

Chain	Residue
A	HIS70
B	ASP84

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site_id	AC2
Number of Residues	2
Details	TWO POLAR RESIDUES ARE FOUND IN THE CATALYTIC REGION OF THE ACTIVE SITE.

Chain	Residue
B	HIS70
A	ASP84

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000305|PubMed:21276098

Chain	Residue	Details
A	PHE71
B	PHE71

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1mka

Chain	Residue	Details
A	ASP84
B	CYS80
B	VAL76
B	GLY79
B	HIS70

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site_id	CSA2
Number of Residues	5
Details	Annotated By Reference To The Literature 1mka

Chain	Residue	Details
A	CYS80
A	VAL76
A	GLY79
A	HIS70
B	ASP84

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site_id	MCSA1
Number of Residues	5
Details	M-CSA 10

Chain	Residue	Details
A	PHE71	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	MET77	activator, electrostatic stabiliser, hydrogen bond acceptor
A	CYS80	electrostatic stabiliser, hydrogen bond donor
A	LEU81	electrostatic stabiliser, hydrogen bond donor
A	ALA85	hydrogen bond donor, proton acceptor, proton donor

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site_id	MCSA2
Number of Residues	5
Details	M-CSA 10

Chain	Residue	Details
B	PHE71	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	MET77	activator, electrostatic stabiliser, hydrogen bond acceptor
B	CYS80	electrostatic stabiliser, hydrogen bond donor
B	LEU81	electrostatic stabiliser, hydrogen bond donor
B	ALA85	hydrogen bond donor, proton acceptor, proton donor

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