Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1MG2

MUTATION OF ALPHA PHE55 OF METHYLAMINE DEHYDROGENASE ALTERS THE REORGANIZATION ENERGY AND ELECTRONIC COUPLING FOR ITS ELECTRON TRANSFER REACTION WITH AMICYANIN

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0030058	molecular_function	aliphatic amine dehydrogenase activity
A	0030416	biological_process	methylamine metabolic process
A	0042597	cellular_component	periplasmic space
B	0009308	biological_process	amine metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
B	0030288	cellular_component	outer membrane-bounded periplasmic space
B	0042597	cellular_component	periplasmic space
B	0052876	molecular_function	methylamine dehydrogenase (amicyanin) activity
C	0005507	molecular_function	copper ion binding
C	0009055	molecular_function	electron transfer activity
C	0042597	cellular_component	periplasmic space
C	0046872	molecular_function	metal ion binding
D	0005506	molecular_function	iron ion binding
D	0009055	molecular_function	electron transfer activity
D	0015945	biological_process	methanol metabolic process
D	0020037	molecular_function	heme binding
D	0042597	cellular_component	periplasmic space
D	0046872	molecular_function	metal ion binding
E	0030058	molecular_function	aliphatic amine dehydrogenase activity
E	0030416	biological_process	methylamine metabolic process
E	0042597	cellular_component	periplasmic space
F	0009308	biological_process	amine metabolic process
F	0016491	molecular_function	oxidoreductase activity
F	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
F	0030288	cellular_component	outer membrane-bounded periplasmic space
F	0042597	cellular_component	periplasmic space
F	0052876	molecular_function	methylamine dehydrogenase (amicyanin) activity
G	0005507	molecular_function	copper ion binding
G	0009055	molecular_function	electron transfer activity
G	0042597	cellular_component	periplasmic space
G	0046872	molecular_function	metal ion binding
H	0005506	molecular_function	iron ion binding
H	0009055	molecular_function	electron transfer activity
H	0015945	biological_process	methanol metabolic process
H	0020037	molecular_function	heme binding
H	0042597	cellular_component	periplasmic space
H	0046872	molecular_function	metal ion binding
I	0030058	molecular_function	aliphatic amine dehydrogenase activity
I	0030416	biological_process	methylamine metabolic process
I	0042597	cellular_component	periplasmic space
J	0009308	biological_process	amine metabolic process
J	0016491	molecular_function	oxidoreductase activity
J	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
J	0030288	cellular_component	outer membrane-bounded periplasmic space
J	0042597	cellular_component	periplasmic space
J	0052876	molecular_function	methylamine dehydrogenase (amicyanin) activity
K	0005507	molecular_function	copper ion binding
K	0009055	molecular_function	electron transfer activity
K	0042597	cellular_component	periplasmic space
K	0046872	molecular_function	metal ion binding
L	0005506	molecular_function	iron ion binding
L	0009055	molecular_function	electron transfer activity
L	0015945	biological_process	methanol metabolic process
L	0020037	molecular_function	heme binding
L	0042597	cellular_component	periplasmic space
L	0046872	molecular_function	metal ion binding
M	0030058	molecular_function	aliphatic amine dehydrogenase activity
M	0030416	biological_process	methylamine metabolic process
M	0042597	cellular_component	periplasmic space
N	0009308	biological_process	amine metabolic process
N	0016491	molecular_function	oxidoreductase activity
N	0016638	molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors
N	0030288	cellular_component	outer membrane-bounded periplasmic space
N	0042597	cellular_component	periplasmic space
N	0052876	molecular_function	methylamine dehydrogenase (amicyanin) activity
O	0005507	molecular_function	copper ion binding
O	0009055	molecular_function	electron transfer activity
O	0042597	cellular_component	periplasmic space
O	0046872	molecular_function	metal ion binding
P	0005506	molecular_function	iron ion binding
P	0009055	molecular_function	electron transfer activity
P	0015945	biological_process	methanol metabolic process
P	0020037	molecular_function	heme binding
P	0042597	cellular_component	periplasmic space
P	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU C 107

Chain	Residue
C	HIS53
C	CYS92
C	HIS95
C	MET98

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU G 107

Chain	Residue
G	HIS53
G	CYS92
G	HIS95
G	MET98

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU K 107

Chain	Residue
K	CYS92
K	HIS95
K	MET98
K	HIS53

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU O 107

Chain	Residue
O	HIS53
O	CYS92
O	HIS95
O	MET98

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 F 401

Chain	Residue
F	PHE110
F	GLY111
F	ASP115
F	HOH1690
F	HOH2285
G	LYS68

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 B 402

Chain	Residue
B	PHE110
B	GLY111
B	ASP115
B	HOH2517
C	LYS68

site_id	AC7
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 J 403

Chain	Residue
J	PHE110
J	GLY111
J	ASP115
J	MET117
J	HOH1065
J	HOH1223
J	HOH1615
J	HOH1902
J	HOH2558
K	LYS68

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 N 404

Chain	Residue
M	HOH2677
N	PHE110
N	GLY111
N	ASP115
N	MET117
N	HOH1282
N	HOH1925
N	HOH2529
O	LYS68

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 I 405

Chain	Residue
I	ALA86
I	ASP87
I	ASP88
I	PRO146
I	ASP147
I	HOH2612

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 E 406

Chain	Residue
E	ALA86
E	ASP87
E	ASP88
E	PRO146
E	GLY148
E	HOH2614

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 M 407

Chain	Residue
G	ASP18
M	ASP87
M	ASP88
M	PRO146
M	GLY148
M	HOH1206

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 A 408

Chain	Residue
A	ALA86
A	ASP87
A	ASP88
A	PRO146

site_id	BC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE NA L 601

Chain	Residue
K	GLU31
K	HOH1348
L	GLY72
L	ASP75
L	TYR77
L	HOH1273
L	HOH1529

site_id	BC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE NA P 602

Chain	Residue
O	GLU31
O	HOH1352
P	GLY72
P	ASP75
P	TYR77
P	HOH1276
P	HOH2639

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA H 603

Chain	Residue
G	GLU31
H	GLY72
H	ASP75
H	TYR77
H	HOH1158

site_id	BC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE NA D 604

Chain	Residue
C	GLU31
D	GLY72
D	ASP75
D	TYR77
D	HOH1002
D	HOH1409
D	HOH1768

site_id	BC8
Number of Residues	16
Details	BINDING SITE FOR RESIDUE HEC D 200

Chain	Residue
D	TRP78
D	THR79
D	TYR80
D	ASN83
D	LEU89
D	LEU93
D	ALA97
D	THR98
D	GLN100
D	MET101
D	HOH1852
D	MET56
D	CYS57
D	CYS60
D	HIS61
D	PRO71

site_id	BC9
Number of Residues	17
Details	BINDING SITE FOR RESIDUE HEC H 200

Chain	Residue
H	MET56
H	CYS57
H	CYS60
H	HIS61
H	PRO71
H	TRP78
H	THR79
H	TYR80
H	ASN83
H	LEU89
H	LEU93
H	ALA97
H	THR98
H	GLN100
H	MET101
H	HOH1646
H	HOH1903

site_id	CC1
Number of Residues	17
Details	BINDING SITE FOR RESIDUE HEC L 200

Chain	Residue
L	MET56
L	CYS57
L	CYS60
L	HIS61
L	PRO71
L	TRP78
L	THR79
L	TYR80
L	ASN83
L	LEU89
L	LEU93
L	ALA97
L	THR98
L	GLN100
L	MET101
L	MET104
L	HOH2369

site_id	CC2
Number of Residues	16
Details	BINDING SITE FOR RESIDUE HEC P 200

Chain	Residue
P	MET56
P	CYS57
P	CYS60
P	HIS61
P	PRO71
P	TRP78
P	THR79
P	TYR80
P	ASN83
P	THR92
P	LEU93
P	ALA97
P	THR98
P	GLN100
P	MET101
P	HOH2639

Functional Information from PROSITE/UniProt

site_id	PS00196
Number of Residues	14
Details	COPPER_BLUE Type-1 copper (blue) proteins signature. AgtYdYHCt.P.Hpf..M

Chain	Residue	Details
C	ALA85-MET98

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Modified residue: {"description":"Tryptophylquinone","evidences":[{"source":"PubMed","id":"1409575","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	416
Details	Domain: {"description":"Plastocyanin-like"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"description":"covalent"}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"description":"axial binding residue"}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 2bbk

Chain	Residue	Details
B	ASP76
B	THR122
B	ASP32
B	TYR119
B	TRP108

site_id	CSA2
Number of Residues	5
Details	Annotated By Reference To The Literature 2bbk

Chain	Residue	Details
F	ASP76
F	THR122
F	ASP32
F	TYR119
F	TRP108

site_id	CSA3
Number of Residues	5
Details	Annotated By Reference To The Literature 2bbk

Chain	Residue	Details
J	ASP76
J	THR122
J	ASP32
J	TYR119
J	TRP108

site_id	CSA4
Number of Residues	5
Details	Annotated By Reference To The Literature 2bbk

Chain	Residue	Details
N	ASP76
N	THR122
N	ASP32
N	TYR119
N	TRP108

site_id	MCSA1
Number of Residues	6
Details	M-CSA 13

Chain	Residue	Details
B	ASP32	electrostatic stabiliser, proton acceptor, proton donor
B	TRQ57	proton acceptor, proton donor, proton relay
B	TYR80	electrostatic stabiliser, proton acceptor, proton donor
B	ALA112	proton acceptor, proton donor, proton relay, single electron donor
B	ILE123	steric role
B	ILE126	electrostatic stabiliser

site_id	MCSA2
Number of Residues	6
Details	M-CSA 13

Chain	Residue	Details
F	ASP32	electrostatic stabiliser, proton acceptor, proton donor
F	TRQ57	proton acceptor, proton donor, proton relay
F	TYR80	electrostatic stabiliser, proton acceptor, proton donor
F	ALA112	proton acceptor, proton donor, proton relay, single electron donor
F	ILE123	steric role
F	ILE126	electrostatic stabiliser

site_id	MCSA3
Number of Residues	6
Details	M-CSA 13

Chain	Residue	Details
J	ASP32	electrostatic stabiliser, proton acceptor, proton donor
J	TRQ57	proton acceptor, proton donor, proton relay
J	TYR80	electrostatic stabiliser, proton acceptor, proton donor
J	ALA112	proton acceptor, proton donor, proton relay, single electron donor
J	ILE123	steric role
J	ILE126	electrostatic stabiliser

site_id	MCSA4
Number of Residues	6
Details	M-CSA 13

Chain	Residue	Details
N	ASP32	electrostatic stabiliser, proton acceptor, proton donor
N	TRQ57	proton acceptor, proton donor, proton relay
N	TYR80	electrostatic stabiliser, proton acceptor, proton donor
N	ALA112	proton acceptor, proton donor, proton relay, single electron donor
N	ILE123	steric role
N	ILE126	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)