1MF0

Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with AMP, GDP, HPO4(2-), and Mg(2+)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003924	molecular_function	GTPase activity
A	0004019	molecular_function	adenylosuccinate synthase activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0006163	biological_process	purine nucleotide metabolic process
A	0006164	biological_process	purine nucleotide biosynthetic process
A	0006167	biological_process	AMP biosynthetic process
A	0006531	biological_process	aspartate metabolic process
A	0016020	cellular_component	membrane
A	0016874	molecular_function	ligase activity
A	0042802	molecular_function	identical protein binding
A	0044208	biological_process	'de novo' AMP biosynthetic process
A	0044209	biological_process	AMP salvage
A	0046040	biological_process	IMP metabolic process
A	0046872	molecular_function	metal ion binding
A	0051015	molecular_function	actin filament binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 1453

Chain	Residue
A	ASP43
A	GLY70
A	PO41451
A	GDP1452

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 A 1451

Chain	Residue
A	HIS71
A	ASN256
A	GDP1452
A	MG1453
A	AMP1454
A	GLY42
A	ASP43
A	LYS46
A	ALA69
A	GLY70

---

site_id	AC3
Number of Residues	16
Details	BINDING SITE FOR RESIDUE AMP A 1454

Chain	Residue
A	ASP43
A	ASN68
A	ALA69
A	GLY70
A	THR162
A	THR163
A	ARG177
A	VAL270
A	THR271
A	ARG304
A	VAL305
A	GLY306
A	HOH520
A	HOH521
A	HOH579
A	PO41451

---

site_id	AC4
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GDP A 1452

Chain	Residue
A	ASP43
A	GLU44
A	GLY45
A	LYS46
A	GLY47
A	LYS48
A	GLY70
A	HIS71
A	THR72
A	LYS363
A	ASP365
A	ILE366
A	GLY445
A	VAL446
A	GLY447
A	LYS448
A	HOH503
A	HOH535
A	PO41451
A	MG1453

---

Functional Information from PROSITE/UniProt

site_id	PS00513
Number of Residues	12
Details	ADENYLOSUCCIN_SYN_2 Adenylosuccinate synthetase active site. GIGPtYssKaaR

Chain	Residue	Details
A	GLY166-ARG177

---

site_id	PS01266
Number of Residues	8
Details	ADENYLOSUCCIN_SYN_1 Adenylosuccinate synthetase GTP-binding site. QWGDEGKG

Chain	Residue	Details
A	GLN40-GLY47

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_03126

Chain	Residue	Details
A	ASP43

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_03126

Chain	Residue	Details
A	HIS71

---

site_id	SWS_FT_FI3
Number of Residues	5
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03126, ECO:0000269|PubMed:12004071

Chain	Residue	Details
A	GLY42
A	ARG177
A	ARG337
A	LYS363
A	GLY445

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	ASP43
A	GLY70
A	VAL331

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: in other chain

Chain	Residue	Details
A	ASN68

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	BINDING: in other chain => ECO:0000255|HAMAP-Rule:MF_03126, ECO:0000269|PubMed:12004071

Chain	Residue	Details
A	THR163
A	ASN256
A	THR271
A	ARG335

---