1M9Y

X-ray crystal structure of Cyclophilin A/HIV-1 CA N-terminal domain (1-146) M-type H87A,G89A Complex.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000413	biological_process	protein peptidyl-prolyl isomerization
A	0001933	biological_process	negative regulation of protein phosphorylation
A	0001934	biological_process	positive regulation of protein phosphorylation
A	0003723	molecular_function	RNA binding
A	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
A	0005178	molecular_function	integrin binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005925	cellular_component	focal adhesion
A	0006457	biological_process	protein folding
A	0006469	biological_process	negative regulation of protein kinase activity
A	0006915	biological_process	apoptotic process
A	0016018	molecular_function	cyclosporin A binding
A	0016020	cellular_component	membrane
A	0016853	molecular_function	isomerase activity
A	0019076	biological_process	viral release from host cell
A	0030168	biological_process	platelet activation
A	0030182	biological_process	neuron differentiation
A	0030593	biological_process	neutrophil chemotaxis
A	0030595	biological_process	leukocyte chemotaxis
A	0031982	cellular_component	vesicle
A	0032148	biological_process	activation of protein kinase B activity
A	0032873	biological_process	negative regulation of stress-activated MAPK cascade
A	0032991	cellular_component	protein-containing complex
A	0034389	biological_process	lipid droplet organization
A	0034599	biological_process	cellular response to oxidative stress
A	0034774	cellular_component	secretory granule lumen
A	0042118	biological_process	endothelial cell activation
A	0043410	biological_process	positive regulation of MAPK cascade
A	0045069	biological_process	regulation of viral genome replication
A	0045070	biological_process	positive regulation of viral genome replication
A	0046790	molecular_function	virion binding
A	0050714	biological_process	positive regulation of protein secretion
A	0051082	molecular_function	unfolded protein binding
A	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
A	0060352	biological_process	cell adhesion molecule production
A	0061944	biological_process	negative regulation of protein K48-linked ubiquitination
A	0070062	cellular_component	extracellular exosome
A	0070527	biological_process	platelet aggregation
A	1902176	biological_process	negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
A	1903901	biological_process	negative regulation of viral life cycle
A	1904399	molecular_function	heparan sulfate binding
A	1904813	cellular_component	ficolin-1-rich granule lumen
A	2001233	biological_process	regulation of apoptotic signaling pathway
B	0000413	biological_process	protein peptidyl-prolyl isomerization
B	0001933	biological_process	negative regulation of protein phosphorylation
B	0001934	biological_process	positive regulation of protein phosphorylation
B	0003723	molecular_function	RNA binding
B	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
B	0005178	molecular_function	integrin binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005925	cellular_component	focal adhesion
B	0006457	biological_process	protein folding
B	0006469	biological_process	negative regulation of protein kinase activity
B	0006915	biological_process	apoptotic process
B	0016018	molecular_function	cyclosporin A binding
B	0016020	cellular_component	membrane
B	0016853	molecular_function	isomerase activity
B	0019076	biological_process	viral release from host cell
B	0030168	biological_process	platelet activation
B	0030182	biological_process	neuron differentiation
B	0030593	biological_process	neutrophil chemotaxis
B	0030595	biological_process	leukocyte chemotaxis
B	0031982	cellular_component	vesicle
B	0032148	biological_process	activation of protein kinase B activity
B	0032873	biological_process	negative regulation of stress-activated MAPK cascade
B	0032991	cellular_component	protein-containing complex
B	0034389	biological_process	lipid droplet organization
B	0034599	biological_process	cellular response to oxidative stress
B	0034774	cellular_component	secretory granule lumen
B	0042118	biological_process	endothelial cell activation
B	0043410	biological_process	positive regulation of MAPK cascade
B	0045069	biological_process	regulation of viral genome replication
B	0045070	biological_process	positive regulation of viral genome replication
B	0046790	molecular_function	virion binding
B	0050714	biological_process	positive regulation of protein secretion
B	0051082	molecular_function	unfolded protein binding
B	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
B	0060352	biological_process	cell adhesion molecule production
B	0061944	biological_process	negative regulation of protein K48-linked ubiquitination
B	0070062	cellular_component	extracellular exosome
B	0070527	biological_process	platelet aggregation
B	1902176	biological_process	negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
B	1903901	biological_process	negative regulation of viral life cycle
B	1904399	molecular_function	heparan sulfate binding
B	1904813	cellular_component	ficolin-1-rich granule lumen
B	2001233	biological_process	regulation of apoptotic signaling pathway
C	0016032	biological_process	viral process
D	0016032	biological_process	viral process
E	0000413	biological_process	protein peptidyl-prolyl isomerization
E	0001933	biological_process	negative regulation of protein phosphorylation
E	0001934	biological_process	positive regulation of protein phosphorylation
E	0003723	molecular_function	RNA binding
E	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
E	0005178	molecular_function	integrin binding
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005925	cellular_component	focal adhesion
E	0006457	biological_process	protein folding
E	0006469	biological_process	negative regulation of protein kinase activity
E	0006915	biological_process	apoptotic process
E	0016018	molecular_function	cyclosporin A binding
E	0016020	cellular_component	membrane
E	0016853	molecular_function	isomerase activity
E	0019076	biological_process	viral release from host cell
E	0030168	biological_process	platelet activation
E	0030182	biological_process	neuron differentiation
E	0030593	biological_process	neutrophil chemotaxis
E	0030595	biological_process	leukocyte chemotaxis
E	0031982	cellular_component	vesicle
E	0032148	biological_process	activation of protein kinase B activity
E	0032873	biological_process	negative regulation of stress-activated MAPK cascade
E	0032991	cellular_component	protein-containing complex
E	0034389	biological_process	lipid droplet organization
E	0034599	biological_process	cellular response to oxidative stress
E	0034774	cellular_component	secretory granule lumen
E	0042118	biological_process	endothelial cell activation
E	0043410	biological_process	positive regulation of MAPK cascade
E	0045069	biological_process	regulation of viral genome replication
E	0045070	biological_process	positive regulation of viral genome replication
E	0046790	molecular_function	virion binding
E	0050714	biological_process	positive regulation of protein secretion
E	0051082	molecular_function	unfolded protein binding
E	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
E	0060352	biological_process	cell adhesion molecule production
E	0061944	biological_process	negative regulation of protein K48-linked ubiquitination
E	0070062	cellular_component	extracellular exosome
E	0070527	biological_process	platelet aggregation
E	1902176	biological_process	negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
E	1903901	biological_process	negative regulation of viral life cycle
E	1904399	molecular_function	heparan sulfate binding
E	1904813	cellular_component	ficolin-1-rich granule lumen
E	2001233	biological_process	regulation of apoptotic signaling pathway
F	0000413	biological_process	protein peptidyl-prolyl isomerization
F	0001933	biological_process	negative regulation of protein phosphorylation
F	0001934	biological_process	positive regulation of protein phosphorylation
F	0003723	molecular_function	RNA binding
F	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
F	0005178	molecular_function	integrin binding
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005615	cellular_component	extracellular space
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005925	cellular_component	focal adhesion
F	0006457	biological_process	protein folding
F	0006469	biological_process	negative regulation of protein kinase activity
F	0006915	biological_process	apoptotic process
F	0016018	molecular_function	cyclosporin A binding
F	0016020	cellular_component	membrane
F	0016853	molecular_function	isomerase activity
F	0019076	biological_process	viral release from host cell
F	0030168	biological_process	platelet activation
F	0030182	biological_process	neuron differentiation
F	0030593	biological_process	neutrophil chemotaxis
F	0030595	biological_process	leukocyte chemotaxis
F	0031982	cellular_component	vesicle
F	0032148	biological_process	activation of protein kinase B activity
F	0032873	biological_process	negative regulation of stress-activated MAPK cascade
F	0032991	cellular_component	protein-containing complex
F	0034389	biological_process	lipid droplet organization
F	0034599	biological_process	cellular response to oxidative stress
F	0034774	cellular_component	secretory granule lumen
F	0042118	biological_process	endothelial cell activation
F	0043410	biological_process	positive regulation of MAPK cascade
F	0045069	biological_process	regulation of viral genome replication
F	0045070	biological_process	positive regulation of viral genome replication
F	0046790	molecular_function	virion binding
F	0050714	biological_process	positive regulation of protein secretion
F	0051082	molecular_function	unfolded protein binding
F	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
F	0060352	biological_process	cell adhesion molecule production
F	0061944	biological_process	negative regulation of protein K48-linked ubiquitination
F	0070062	cellular_component	extracellular exosome
F	0070527	biological_process	platelet aggregation
F	1902176	biological_process	negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
F	1903901	biological_process	negative regulation of viral life cycle
F	1904399	molecular_function	heparan sulfate binding
F	1904813	cellular_component	ficolin-1-rich granule lumen
F	2001233	biological_process	regulation of apoptotic signaling pathway
G	0016032	biological_process	viral process
H	0016032	biological_process	viral process

Functional Information from PROSITE/UniProt

site_id	PS00170
Number of Residues	18
Details	CSA_PPIASE_1 Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature. YkgScFHRIIpgFMcQGG

Chain	Residue	Details
A	TYR48-GLY65

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	624
Details	Domain: {"description":"PPIase cyclophilin-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00156","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22223895","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25944712","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Modified residue: {"description":"N-acetylvaline; partial; in Peptidyl-prolyl cis-trans isomerase A, N-terminally processed","evidences":[{"source":"PubMed","id":"25489052","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2005","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Claeys D."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25944712","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"20364129","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25678563","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P17742","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate"}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 189

Chain	Residue	Details
A	ARG55	electrostatic stabiliser, hydrogen bond donor, steric role
A	PHE60	polar/non-polar interaction, steric role
A	GLN63	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
A	ASN102	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
A	PHE113	polar/non-polar interaction, steric role
A	LEU122	polar/non-polar interaction, steric role
A	HIS126	polar/non-polar interaction, steric role

---

site_id	MCSA2
Number of Residues	7
Details	M-CSA 189

Chain	Residue	Details
B	ARG55	electrostatic stabiliser, hydrogen bond donor, steric role
B	PHE60	polar/non-polar interaction, steric role
B	GLN63	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
B	ASN102	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
B	PHE113	polar/non-polar interaction, steric role
B	LEU122	polar/non-polar interaction, steric role
B	HIS126	polar/non-polar interaction, steric role

---

site_id	MCSA3
Number of Residues	7
Details	M-CSA 189

Chain	Residue	Details
E	ARG55	electrostatic stabiliser, hydrogen bond donor, steric role
E	PHE60	polar/non-polar interaction, steric role
E	GLN63	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
E	ASN102	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
E	PHE113	polar/non-polar interaction, steric role
E	LEU122	polar/non-polar interaction, steric role
E	HIS126	polar/non-polar interaction, steric role

---

site_id	MCSA4
Number of Residues	7
Details	M-CSA 189

Chain	Residue	Details
F	ARG55	electrostatic stabiliser, hydrogen bond donor, steric role
F	PHE60	polar/non-polar interaction, steric role
F	GLN63	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
F	ASN102	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
F	PHE113	polar/non-polar interaction, steric role
F	LEU122	polar/non-polar interaction, steric role
F	HIS126	polar/non-polar interaction, steric role

---