1M3Z
Biosynthetic thiolase, C89A mutant, complexed with acetyl coenzyme A
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| A | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| A | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| A | 0044281 | biological_process | small molecule metabolic process |
| B | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| B | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| B | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| B | 0044281 | biological_process | small molecule metabolic process |
| C | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| C | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016746 | molecular_function | acyltransferase activity |
| C | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| C | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| C | 0044281 | biological_process | small molecule metabolic process |
| D | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| D | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016746 | molecular_function | acyltransferase activity |
| D | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| D | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| D | 0044281 | biological_process | small molecule metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 9720 |
| Chain | Residue |
| A | SER260 |
| A | ARG266 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 9722 |
| Chain | Residue |
| A | LYS298 |
| A | ARG302 |
| A | HOH9908 |
| site_id | AC3 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE ACO A 1393 |
| Chain | Residue |
| A | ARG220 |
| A | SER227 |
| A | MET228 |
| A | ALA243 |
| A | SER247 |
| A | MET288 |
| A | PHE319 |
| A | HIS348 |
| A | CYS378 |
| A | ILE379 |
| A | GLY380 |
| A | HOH395 |
| A | HOH443 |
| A | HOH445 |
| A | HOH449 |
| A | HOH9751 |
| A | HOH9793 |
| A | HOH9820 |
| A | HOH9860 |
| A | HOH9915 |
| D | MET134 |
| A | ALA89 |
| A | LEU148 |
| A | HIS156 |
| A | MET157 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 9719 |
| Chain | Residue |
| B | LYS298 |
| B | ARG302 |
| B | HOH9952 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 9721 |
| Chain | Residue |
| B | SER260 |
| B | ARG266 |
| B | HOH441 |
| B | HOH456 |
| B | HOH9856 |
| site_id | AC6 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE ACO B 2393 |
| Chain | Residue |
| B | ALA89 |
| B | LEU148 |
| B | HIS156 |
| B | MET157 |
| B | ARG220 |
| B | SER227 |
| B | MET228 |
| B | ALA243 |
| B | GLY244 |
| B | SER247 |
| B | MET288 |
| B | ALA318 |
| B | PHE319 |
| B | HIS348 |
| B | CYS378 |
| B | ILE379 |
| B | HOH394 |
| B | HOH421 |
| B | HOH448 |
| B | HOH9757 |
| B | HOH9776 |
| B | HOH9790 |
| B | HOH9907 |
| B | HOH9910 |
| site_id | AC7 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE ACO C 3393 |
| Chain | Residue |
| A | LYS208 |
| B | MET134 |
| C | ALA89 |
| C | LEU148 |
| C | HIS156 |
| C | MET157 |
| C | ARG220 |
| C | SER227 |
| C | MET228 |
| C | LEU231 |
| C | ALA243 |
| C | GLY244 |
| C | SER247 |
| C | MET288 |
| C | ALA318 |
| C | PHE319 |
| C | HIS348 |
| C | CYS378 |
| C | ILE379 |
| C | HOH3496 |
| C | HOH3551 |
| C | HOH3562 |
| site_id | AC8 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE ACO D 4393 |
| Chain | Residue |
| D | GLY380 |
| D | HOH4497 |
| D | HOH4554 |
| D | HOH4561 |
| D | HOH4569 |
| D | HOH4570 |
| D | HOH4576 |
| D | ALA89 |
| D | LEU148 |
| D | HIS156 |
| D | ARG220 |
| D | SER227 |
| D | MET228 |
| D | ALA243 |
| D | GLY244 |
| D | SER247 |
| D | MET288 |
| D | ALA318 |
| D | PHE319 |
| D | HIS348 |
| D | CYS378 |
| D | ILE379 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Acyl-thioester intermediate"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Active site: {"description":"Proton acceptor"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| A | HIS348 | |
| A | CYS378 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| B | HIS348 | |
| B | CYS378 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| C | HIS348 | |
| C | CYS378 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| D | HIS348 | |
| D | CYS378 |
