1LVU
Crystal structure of calf spleen purine nucleoside phosphorylase in a new space group with full trimer in the asymmetric unit
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| A | 0006166 | biological_process | purine ribonucleoside salvage |
| A | 0009116 | biological_process | nucleoside metabolic process |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0016763 | molecular_function | pentosyltransferase activity |
| A | 0047975 | molecular_function | guanosine phosphorylase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| B | 0006166 | biological_process | purine ribonucleoside salvage |
| B | 0009116 | biological_process | nucleoside metabolic process |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0016763 | molecular_function | pentosyltransferase activity |
| B | 0047975 | molecular_function | guanosine phosphorylase activity |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| C | 0006166 | biological_process | purine ribonucleoside salvage |
| C | 0009116 | biological_process | nucleoside metabolic process |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0016763 | molecular_function | pentosyltransferase activity |
| C | 0047975 | molecular_function | guanosine phosphorylase activity |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| D | 0006166 | biological_process | purine ribonucleoside salvage |
| D | 0009116 | biological_process | nucleoside metabolic process |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0016763 | molecular_function | pentosyltransferase activity |
| D | 0047975 | molecular_function | guanosine phosphorylase activity |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| E | 0006166 | biological_process | purine ribonucleoside salvage |
| E | 0009116 | biological_process | nucleoside metabolic process |
| E | 0016757 | molecular_function | glycosyltransferase activity |
| E | 0016763 | molecular_function | pentosyltransferase activity |
| E | 0047975 | molecular_function | guanosine phosphorylase activity |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| F | 0006166 | biological_process | purine ribonucleoside salvage |
| F | 0009116 | biological_process | nucleoside metabolic process |
| F | 0016757 | molecular_function | glycosyltransferase activity |
| F | 0016763 | molecular_function | pentosyltransferase activity |
| F | 0047975 | molecular_function | guanosine phosphorylase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 8001 |
| Chain | Residue |
| A | SER51 |
| A | GLU58 |
| A | HOH8018 |
| A | HOH8022 |
| A | HOH8094 |
| D | GLU58 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA D 8002 |
| Chain | Residue |
| D | HOH8044 |
| D | HOH8058 |
| D | HOH8154 |
| A | GLU58 |
| D | SER51 |
| D | GLU58 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA F 8003 |
| Chain | Residue |
| B | GLU1058 |
| B | HOH8073 |
| F | HOH204 |
| F | HOH306 |
| F | SER2051 |
| F | GLU2058 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA B 8004 |
| Chain | Residue |
| B | SER1051 |
| B | GLU1058 |
| B | HOH8022 |
| B | HOH8052 |
| B | HOH8071 |
| F | GLU2058 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA C 8005 |
| Chain | Residue |
| C | HOH117 |
| C | HOH355 |
| C | SER2051 |
| C | GLU2058 |
| E | GLU1058 |
| E | HOH8072 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA E 8006 |
| Chain | Residue |
| C | GLU2058 |
| E | SER1051 |
| E | GLU1058 |
| E | HOH8042 |
| E | HOH8059 |
| E | HOH8090 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA B 8007 |
| Chain | Residue |
| B | ASP1215 |
| B | HOH8043 |
| B | HOH8244 |
| B | HOH8337 |
| D | GLU250 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA F 8008 |
| Chain | Residue |
| C | GLU2250 |
| F | HOH76 |
| F | HOH582 |
| F | ASN2121 |
| F | ASP2215 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 8009 |
| Chain | Residue |
| A | GLU8 |
| A | HOH8082 |
| A | HOH8091 |
| E | GLU1008 |
| E | HOH8032 |
| E | HOH8274 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA B 8010 |
| Chain | Residue |
| B | GLU1008 |
| B | HOH8037 |
| B | HOH8131 |
| D | GLU8 |
| D | HOH8033 |
| D | HOH8131 |
| site_id | BC2 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE 9PP A 6100 |
| Chain | Residue |
| A | GLY32 |
| A | SER33 |
| A | HIS86 |
| A | ASN115 |
| A | ALA116 |
| A | GLY118 |
| A | LEU195 |
| A | PHE200 |
| A | GLU201 |
| A | VAL217 |
| A | GLY218 |
| A | MET219 |
| A | SER220 |
| A | ASN243 |
| A | HOH8034 |
| A | HOH8048 |
| A | HOH8070 |
| A | HOH8092 |
| A | HOH8131 |
| A | HOH8162 |
| site_id | BC3 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE 9PP B 6200 |
| Chain | Residue |
| B | GLY1032 |
| B | SER1033 |
| B | ARG1084 |
| B | HIS1086 |
| B | ASN1115 |
| B | ALA1116 |
| B | ALA1117 |
| B | GLY1118 |
| B | LEU1195 |
| B | PHE1200 |
| B | GLU1201 |
| B | VAL1217 |
| B | GLY1218 |
| B | MET1219 |
| B | SER1220 |
| B | ASN1243 |
| B | HOH8012 |
| B | HOH8057 |
| B | HOH8092 |
| B | HOH8105 |
| B | HOH8119 |
| site_id | BC4 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE 9PP C 6300 |
| Chain | Residue |
| C | HOH134 |
| C | HOH326 |
| C | HOH483 |
| C | HOH707 |
| C | HOH1653 |
| C | GLY2032 |
| C | SER2033 |
| C | ARG2084 |
| C | HIS2086 |
| C | ASN2115 |
| C | ALA2116 |
| C | ALA2117 |
| C | GLY2118 |
| C | PHE2200 |
| C | GLU2201 |
| C | VAL2217 |
| C | GLY2218 |
| C | MET2219 |
| C | SER2220 |
| C | ASN2243 |
| site_id | BC5 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE 9PP D 6400 |
| Chain | Residue |
| D | GLY32 |
| D | SER33 |
| D | HIS86 |
| D | ASN115 |
| D | ALA116 |
| D | ALA117 |
| D | GLY118 |
| D | LEU195 |
| D | PHE200 |
| D | GLU201 |
| D | VAL217 |
| D | GLY218 |
| D | MET219 |
| D | SER220 |
| D | ASN243 |
| D | HOH8019 |
| D | HOH8024 |
| D | HOH8056 |
| D | HOH8083 |
| D | HOH8123 |
| site_id | BC6 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE 9PP E 6500 |
| Chain | Residue |
| E | GLY1032 |
| E | SER1033 |
| E | ARG1084 |
| E | HIS1086 |
| E | ASN1115 |
| E | ALA1116 |
| E | ALA1117 |
| E | GLY1118 |
| E | LEU1195 |
| E | PHE1200 |
| E | GLU1201 |
| E | VAL1217 |
| E | GLY1218 |
| E | MET1219 |
| E | SER1220 |
| E | ASN1243 |
| E | HOH8016 |
| E | HOH8022 |
| E | HOH8030 |
| E | HOH8051 |
| E | HOH8056 |
| F | PHE2159 |
| site_id | BC7 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE 9PP F 6600 |
| Chain | Residue |
| F | HOH157 |
| F | HOH276 |
| F | HOH279 |
| F | HOH860 |
| F | GLY2032 |
| F | SER2033 |
| F | HIS2086 |
| F | ASN2115 |
| F | ALA2116 |
| F | ALA2117 |
| F | GLY2118 |
| F | LEU2195 |
| F | PHE2200 |
| F | GLU2201 |
| F | VAL2217 |
| F | GLY2218 |
| F | MET2219 |
| F | SER2220 |
| F | ASN2243 |
Functional Information from PROSITE/UniProt
| site_id | PS01240 |
| Number of Residues | 42 |
| Details | PNP_MTAP_2 Purine and other phosphorylases family 2 signature. VmmqGrfHmYegypFwkvTfpVrVfrllGvet.LVvtNAaGGL |
| Chain | Residue | Details |
| A | VAL79-LEU120 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0007744|PDB:1A9O, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9S, ECO:0007744|PDB:1A9T, ECO:0007744|PDB:3PNP, ECO:0007744|PDB:4PNP |
| Chain | Residue | Details |
| A | SER33 | |
| B | SER1033 | |
| C | SER2033 | |
| D | SER33 | |
| E | SER1033 | |
| F | SER2033 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9T, ECO:0007744|PDB:3PNP, ECO:0007744|PDB:4PNP |
| Chain | Residue | Details |
| A | HIS64 | |
| B | HIS1064 | |
| C | HIS2064 | |
| D | HIS64 | |
| E | HIS1064 | |
| F | HIS2064 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 18 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0007744|PDB:1A9O, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9Q, ECO:0007744|PDB:1A9R, ECO:0007744|PDB:1A9S, ECO:0007744|PDB:1A9T, ECO:0007744|PDB:3PNP, ECO:0007744|PDB:4PNP |
| Chain | Residue | Details |
| A | ARG84 | |
| D | ARG84 | |
| D | ALA116 | |
| D | SER220 | |
| E | ARG1084 | |
| E | ALA1116 | |
| E | SER1220 | |
| F | ARG2084 | |
| F | ALA2116 | |
| F | SER2220 | |
| A | ALA116 | |
| A | SER220 | |
| B | ARG1084 | |
| B | ALA1116 | |
| B | SER1220 | |
| C | ARG2084 | |
| C | ALA2116 | |
| C | SER2220 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 18 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9S, ECO:0007744|PDB:1A9T |
| Chain | Residue | Details |
| A | TYR88 | |
| D | TYR88 | |
| D | MET219 | |
| D | HIS257 | |
| E | TYR1088 | |
| E | MET1219 | |
| E | HIS1257 | |
| F | TYR2088 | |
| F | MET2219 | |
| F | HIS2257 | |
| A | MET219 | |
| A | HIS257 | |
| B | TYR1088 | |
| B | MET1219 | |
| B | HIS1257 | |
| C | TYR2088 | |
| C | MET2219 | |
| C | HIS2257 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0000305|PubMed:9020983, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9Q, ECO:0007744|PDB:1A9R, ECO:0007744|PDB:1VFN |
| Chain | Residue | Details |
| A | GLU201 | |
| E | ASN1243 | |
| F | GLU2201 | |
| F | ASN2243 | |
| A | ASN243 | |
| B | GLU1201 | |
| B | ASN1243 | |
| C | GLU2201 | |
| C | ASN2243 | |
| D | GLU201 | |
| D | ASN243 | |
| E | GLU1201 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | SITE: Important for substrate specificity => ECO:0000250|UniProtKB:P00491 |
| Chain | Residue | Details |
| A | ASN243 | |
| B | ASN1243 | |
| C | ASN2243 | |
| D | ASN243 | |
| E | ASN1243 | |
| F | ASN2243 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | MOD_RES: N-acetylmethionine => ECO:0000250|UniProtKB:P00491 |
| Chain | Residue | Details |
| A | MET1 | |
| B | MET1001 | |
| C | MET2001 | |
| D | MET1 | |
| E | MET1001 | |
| F | MET2001 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P00491 |
| Chain | Residue | Details |
| A | SER251 | |
| B | SER1251 | |
| C | SER2251 | |
| D | SER251 | |
| E | SER1251 | |
| F | SER2251 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| A | ASN243 | |
| A | HIS86 | |
| A | GLU89 |
| site_id | CSA10 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| D | ASN243 | |
| D | ASP248 |
| site_id | CSA11 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| E | ASP1248 | |
| E | ASN1243 |
| site_id | CSA12 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| F | ASN2243 | |
| F | ASP2248 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| B | ASN1243 | |
| B | GLU1089 | |
| B | HIS1086 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| C | HIS2086 | |
| C | ASN2243 | |
| C | GLU2089 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| D | ASN243 | |
| D | HIS86 | |
| D | GLU89 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| E | ASN1243 | |
| E | GLU1089 | |
| E | HIS1086 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| F | HIS2086 | |
| F | ASN2243 | |
| F | GLU2089 |
| site_id | CSA7 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| A | ASN243 | |
| A | ASP248 |
| site_id | CSA8 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| B | ASP1248 | |
| B | ASN1243 |
| site_id | CSA9 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| C | ASN2243 | |
| C | ASP2248 |
