1LV8
Crystal structure of calf spleen purine nucleoside phosphorylase in a new space group with full trimer in the asymmetric unit
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| A | 0006166 | biological_process | purine ribonucleoside salvage |
| A | 0009116 | biological_process | nucleoside metabolic process |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0016763 | molecular_function | pentosyltransferase activity |
| A | 0047975 | molecular_function | guanosine phosphorylase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| B | 0006166 | biological_process | purine ribonucleoside salvage |
| B | 0009116 | biological_process | nucleoside metabolic process |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0016763 | molecular_function | pentosyltransferase activity |
| B | 0047975 | molecular_function | guanosine phosphorylase activity |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| C | 0006166 | biological_process | purine ribonucleoside salvage |
| C | 0009116 | biological_process | nucleoside metabolic process |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0016763 | molecular_function | pentosyltransferase activity |
| C | 0047975 | molecular_function | guanosine phosphorylase activity |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| D | 0006166 | biological_process | purine ribonucleoside salvage |
| D | 0009116 | biological_process | nucleoside metabolic process |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0016763 | molecular_function | pentosyltransferase activity |
| D | 0047975 | molecular_function | guanosine phosphorylase activity |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| E | 0006166 | biological_process | purine ribonucleoside salvage |
| E | 0009116 | biological_process | nucleoside metabolic process |
| E | 0016757 | molecular_function | glycosyltransferase activity |
| E | 0016763 | molecular_function | pentosyltransferase activity |
| E | 0047975 | molecular_function | guanosine phosphorylase activity |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| F | 0006166 | biological_process | purine ribonucleoside salvage |
| F | 0009116 | biological_process | nucleoside metabolic process |
| F | 0016757 | molecular_function | glycosyltransferase activity |
| F | 0016763 | molecular_function | pentosyltransferase activity |
| F | 0047975 | molecular_function | guanosine phosphorylase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA D 7001 |
| Chain | Residue |
| A | GLU58 |
| D | SER51 |
| D | GLU58 |
| D | HOH7032 |
| D | HOH7034 |
| D | HOH7100 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 7002 |
| Chain | Residue |
| A | HOH7069 |
| D | GLU58 |
| D | HOH7085 |
| A | SER51 |
| A | GLU58 |
| A | HOH7052 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA E 7003 |
| Chain | Residue |
| C | GLU2058 |
| E | HOH155 |
| E | HOH439 |
| E | HOH693 |
| E | SER1051 |
| E | GLU1058 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA C 7004 |
| Chain | Residue |
| C | HOH262 |
| C | HOH305 |
| C | HOH718 |
| C | SER2051 |
| C | GLU2058 |
| E | GLU1058 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA B 7005 |
| Chain | Residue |
| B | HOH363 |
| B | SER1051 |
| B | GLU1058 |
| F | HOH376 |
| F | HOH588 |
| F | GLU2058 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA F 7006 |
| Chain | Residue |
| B | GLU1058 |
| F | HOH207 |
| F | HOH287 |
| F | HOH368 |
| F | SER2051 |
| F | GLU2058 |
| site_id | AC7 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE 9PP A 3001 |
| Chain | Residue |
| A | GLY32 |
| A | SER33 |
| A | HIS86 |
| A | ASN115 |
| A | ALA116 |
| A | ALA117 |
| A | GLY118 |
| A | LEU195 |
| A | PHE200 |
| A | GLU201 |
| A | GLY218 |
| A | MET219 |
| A | SER220 |
| A | ASN243 |
| A | HOH7033 |
| A | HOH7050 |
| A | HOH7122 |
| site_id | AC8 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE 9PP B 3002 |
| Chain | Residue |
| B | HOH105 |
| B | HOH173 |
| B | HOH319 |
| B | HOH322 |
| B | GLY1032 |
| B | SER1033 |
| B | HIS1086 |
| B | ASN1115 |
| B | ALA1116 |
| B | ALA1117 |
| B | GLY1118 |
| B | LEU1195 |
| B | PHE1200 |
| B | GLU1201 |
| B | VAL1217 |
| B | GLY1218 |
| B | MET1219 |
| B | SER1220 |
| B | ASN1243 |
| site_id | AC9 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE 9PP C 3003 |
| Chain | Residue |
| C | HOH96 |
| C | HOH103 |
| C | HOH460 |
| C | HOH464 |
| C | GLY2032 |
| C | SER2033 |
| C | HIS2086 |
| C | ASN2115 |
| C | ALA2116 |
| C | ALA2117 |
| C | GLY2118 |
| C | LEU2195 |
| C | PHE2200 |
| C | GLU2201 |
| C | VAL2217 |
| C | GLY2218 |
| C | MET2219 |
| C | SER2220 |
| C | ASN2243 |
| site_id | BC1 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE 9PP E 3004 |
| Chain | Residue |
| E | ALA1116 |
| E | LEU1195 |
| E | PHE1200 |
| E | GLU1201 |
| E | VAL1217 |
| E | GLY1218 |
| E | MET1219 |
| E | SER1220 |
| E | ASN1243 |
| E | HOH222 |
| E | HOH236 |
| E | HOH620 |
| E | HOH674 |
| E | GLY1032 |
| E | SER1033 |
| E | HIS1086 |
| E | ASN1115 |
| site_id | BC2 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE 9PP F 3005 |
| Chain | Residue |
| F | HOH138 |
| F | HOH150 |
| F | HOH338 |
| F | HOH691 |
| F | GLY2032 |
| F | SER2033 |
| F | HIS2086 |
| F | ASN2115 |
| F | ALA2116 |
| F | ALA2117 |
| F | GLY2118 |
| F | LEU2195 |
| F | PHE2200 |
| F | GLU2201 |
| F | VAL2217 |
| F | GLY2218 |
| F | MET2219 |
| F | SER2220 |
| F | ASN2243 |
| site_id | BC3 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE 9PP D 3006 |
| Chain | Residue |
| D | GLY32 |
| D | SER33 |
| D | HIS86 |
| D | ASN115 |
| D | ALA116 |
| D | ALA117 |
| D | GLY118 |
| D | LEU195 |
| D | PHE200 |
| D | GLU201 |
| D | MET219 |
| D | SER220 |
| D | ASN243 |
| D | HOH7044 |
| D | HOH7055 |
| D | HOH7058 |
| D | HOH7068 |
Functional Information from PROSITE/UniProt
| site_id | PS01240 |
| Number of Residues | 42 |
| Details | PNP_MTAP_2 Purine and other phosphorylases family 2 signature. VmmqGrfHmYegypFwkvTfpVrVfrllGvet.LVvtNAaGGL |
| Chain | Residue | Details |
| A | VAL79-LEU120 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0007744|PDB:1A9O, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9S, ECO:0007744|PDB:1A9T, ECO:0007744|PDB:3PNP, ECO:0007744|PDB:4PNP |
| Chain | Residue | Details |
| A | SER33 | |
| B | SER1033 | |
| C | SER2033 | |
| D | SER33 | |
| E | SER1033 | |
| F | SER2033 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9T, ECO:0007744|PDB:3PNP, ECO:0007744|PDB:4PNP |
| Chain | Residue | Details |
| A | HIS64 | |
| B | HIS1064 | |
| C | HIS2064 | |
| D | HIS64 | |
| E | HIS1064 | |
| F | HIS2064 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 18 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0007744|PDB:1A9O, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9Q, ECO:0007744|PDB:1A9R, ECO:0007744|PDB:1A9S, ECO:0007744|PDB:1A9T, ECO:0007744|PDB:3PNP, ECO:0007744|PDB:4PNP |
| Chain | Residue | Details |
| A | ARG84 | |
| D | ARG84 | |
| D | ALA116 | |
| D | SER220 | |
| E | ARG1084 | |
| E | ALA1116 | |
| E | SER1220 | |
| F | ARG2084 | |
| F | ALA2116 | |
| F | SER2220 | |
| A | ALA116 | |
| A | SER220 | |
| B | ARG1084 | |
| B | ALA1116 | |
| B | SER1220 | |
| C | ARG2084 | |
| C | ALA2116 | |
| C | SER2220 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 18 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9S, ECO:0007744|PDB:1A9T |
| Chain | Residue | Details |
| A | TYR88 | |
| D | TYR88 | |
| D | MET219 | |
| D | HIS257 | |
| E | TYR1088 | |
| E | MET1219 | |
| E | HIS1257 | |
| F | TYR2088 | |
| F | MET2219 | |
| F | HIS2257 | |
| A | MET219 | |
| A | HIS257 | |
| B | TYR1088 | |
| B | MET1219 | |
| B | HIS1257 | |
| C | TYR2088 | |
| C | MET2219 | |
| C | HIS2257 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9585525, ECO:0000305|PubMed:9020983, ECO:0007744|PDB:1A9P, ECO:0007744|PDB:1A9Q, ECO:0007744|PDB:1A9R, ECO:0007744|PDB:1VFN |
| Chain | Residue | Details |
| A | GLU201 | |
| E | ASN1243 | |
| F | GLU2201 | |
| F | ASN2243 | |
| A | ASN243 | |
| B | GLU1201 | |
| B | ASN1243 | |
| C | GLU2201 | |
| C | ASN2243 | |
| D | GLU201 | |
| D | ASN243 | |
| E | GLU1201 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | SITE: Important for substrate specificity => ECO:0000250|UniProtKB:P00491 |
| Chain | Residue | Details |
| A | ASN243 | |
| B | ASN1243 | |
| C | ASN2243 | |
| D | ASN243 | |
| E | ASN1243 | |
| F | ASN2243 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | MOD_RES: N-acetylmethionine => ECO:0000250|UniProtKB:P00491 |
| Chain | Residue | Details |
| A | MET1 | |
| B | MET1001 | |
| C | MET2001 | |
| D | MET1 | |
| E | MET1001 | |
| F | MET2001 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P00491 |
| Chain | Residue | Details |
| A | SER251 | |
| B | SER1251 | |
| C | SER2251 | |
| D | SER251 | |
| E | SER1251 | |
| F | SER2251 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| A | ASN243 | |
| A | HIS86 | |
| A | GLU89 |
| site_id | CSA10 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| D | ASN243 | |
| D | ASP248 |
| site_id | CSA11 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| E | ASP1248 | |
| E | ASN1243 |
| site_id | CSA12 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| F | ASN2243 | |
| F | ASP2248 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| B | ASN1243 | |
| B | GLU1089 | |
| B | HIS1086 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| C | HIS2086 | |
| C | ASN2243 | |
| C | GLU2089 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| D | ASN243 | |
| D | HIS86 | |
| D | GLU89 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| E | ASN1243 | |
| E | GLU1089 | |
| E | HIS1086 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| F | HIS2086 | |
| F | ASN2243 | |
| F | GLU2089 |
| site_id | CSA7 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| A | ASN243 | |
| A | ASP248 |
| site_id | CSA8 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| B | ASP1248 | |
| B | ASN1243 |
| site_id | CSA9 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cg6 |
| Chain | Residue | Details |
| C | ASN2243 | |
| C | ASP2248 |
