Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0050661 | molecular_function | NADP binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE NDP A 168 |
| Chain | Residue |
| A | TRP5 |
| A | THR45 |
| A | LEU62 |
| A | THR63 |
| A | HIS64 |
| A | GLN65 |
| A | ALA97 |
| A | GLY99 |
| A | ALA100 |
| A | GLN101 |
| A | ILE102 |
| A | ALA6 |
| A | TRR170 |
| A | ILE13 |
| A | GLY14 |
| A | LYS15 |
| A | GLY17 |
| A | GLY42 |
| A | ARG43 |
| A | ARG44 |
| site_id | AC2 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE TRR A 170 |
| Chain | Residue |
| A | LEU4 |
| A | TRP5 |
| A | ALA6 |
| A | GLY17 |
| A | LEU19 |
| A | ASP26 |
| A | LEU27 |
| A | PHE30 |
| A | PHE49 |
| A | PRO50 |
| A | ALA97 |
| A | THR116 |
| A | NDP168 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. LIGkdghLPWhlpd.DlhyFraqT |
| Chain | Residue | Details |
| A | LEU12-THR34 | |
Functional Information from SwissProt/UniProt
| Chain | Residue | Details |
| A | TRP5 | |
| A | LEU27 | |
| A | ALA32 | |
| A | THR58 | |
| A | ARG117 | |
| Chain | Residue | Details |
| A | ALA6 | |
| A | GLY14 | |
| A | ARG43 | |
| A | THR63 | |
| A | ALA80 | |
| A | GLY98 | |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | SITE: May be important for enzyme function |
| Chain | Residue | Details |
| A | HIS22 | |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1dhf |
| Chain | Residue | Details |
| A | LEU19 | |
| A | ASP26 | |
| site_id | MCSA1 |
| Number of Residues | 9 |
| Details | M-CSA 112 |
| Chain | Residue | Details |
| A | TRP5 | steric role |
| A | PRO20 | enhance reactivity |
| A | HIS22 | activator, hydrogen bond donor |
| A | LEU27 | hydrogen bond acceptor, modifies pKa |
| A | HIS28 | steric locator |
| A | ARG31 | steric locator |
| A | PRO55 | steric locator |
| A | VAL95 | steric role |
| A | ARG117 | electrostatic stabiliser, hydrogen bond donor |
