1LTH

T AND R STATES IN THE CRYSTALS OF BACTERIAL L-LACTATE DEHYDROGENASE REVEAL THE MECHANISM FOR ALLOSTERIC CONTROL

Functional Information from GO Data

Chain	GOid	namespace	contents
R	0003824	molecular_function	catalytic activity
R	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
R	0005737	cellular_component	cytoplasm
R	0006089	biological_process	lactate metabolic process
R	0006096	biological_process	glycolytic process
R	0016491	molecular_function	oxidoreductase activity
R	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
R	0019752	biological_process	carboxylic acid metabolic process
T	0003824	molecular_function	catalytic activity
T	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
T	0005737	cellular_component	cytoplasm
T	0006089	biological_process	lactate metabolic process
T	0006096	biological_process	glycolytic process
T	0016491	molecular_function	oxidoreductase activity
T	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
T	0019752	biological_process	carboxylic acid metabolic process

Functional Information from PDB Data

site_id	RFB
Number of Residues	5
Details	FBP BINDING SITE IN THE R CHAIN

Chain	Residue
R	ARG158
R	LYS170
R	ASN171
R	HIS173
R	TYR175

---

site_id	RND
Number of Residues	18
Details	NADH BINDING SITE IN THE R CHAIN

Chain	Residue
R	ILE13
R	GLY16
R	ALA17
R	VAL18
R	ASP39
R	ILE40
R	THR82
R	ALA83
R	GLY84
R	PRO85
R	ARG86
R	ILE103
R	ILE107
R	ILE123
R	THR124
R	ASN125
R	HIS180
R	ILE240

---

site_id	ROX
Number of Residues	8
Details	OXAMATE BINDING SITE IN THE R CHAIN

Chain	Residue
R	GLN87
R	ARG93
R	ASN125
R	LEU152
R	ARG156
R	HIS180
R	ALA226
R	THR236

---

site_id	TFB
Number of Residues	5
Details	FBP BINDING SITE IN THE T CHAIN

Chain	Residue
T	ARG158
T	LYS170
T	ASN171
T	HIS173
T	TYR175

---

site_id	TND
Number of Residues	20
Details	NADH BINDING SITE IN THE T CHAIN

Chain	Residue
T	ILE13
T	GLY84
T	ARG86
T	ILE103
T	ILE107
T	ILE123
T	THR124
T	ASN125
T	HIS180
T	ILE230
T	ASN237
T	GLY16
T	ILE240
T	ALA17
T	VAL18
T	ASP39
T	ILE40
T	ARG44
T	THR82
T	ALA83

---

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. AGEHGDS

Chain	Residue	Details
T	ALA177-SER183

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"7656036","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1LTH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"7656036","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8450537","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LLD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1LTH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7656036","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8450537","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LLD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1LTH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"7656036","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1LTH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"7656036","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LTH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7656036","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LTH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
T	HIS180
T	ASP153

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
R	HIS180
R	ASP153

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
T	ARG156
T	HIS180
T	ASP153

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
R	ARG156
R	HIS180
R	ASP153

---