1LFL

DEOXY HEMOGLOBIN (90% RELATIVE HUMIDITY)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004601	molecular_function	peroxidase activity
A	0005344	molecular_function	oxygen carrier activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005829	cellular_component	cytosol
A	0005833	cellular_component	hemoglobin complex
A	0006954	biological_process	inflammatory response
A	0015670	biological_process	carbon dioxide transport
A	0015671	biological_process	oxygen transport
A	0016020	cellular_component	membrane
A	0019825	molecular_function	oxygen binding
A	0020037	molecular_function	heme binding
A	0030185	biological_process	nitric oxide transport
A	0031720	molecular_function	haptoglobin binding
A	0031838	cellular_component	haptoglobin-hemoglobin complex
A	0042542	biological_process	response to hydrogen peroxide
A	0042744	biological_process	hydrogen peroxide catabolic process
A	0046872	molecular_function	metal ion binding
A	0048821	biological_process	erythrocyte development
A	0070062	cellular_component	extracellular exosome
A	0071682	cellular_component	endocytic vesicle lumen
A	0072562	cellular_component	blood microparticle
A	0098869	biological_process	cellular oxidant detoxification
B	0004601	molecular_function	peroxidase activity
B	0005344	molecular_function	oxygen carrier activity
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005829	cellular_component	cytosol
B	0005833	cellular_component	hemoglobin complex
B	0006954	biological_process	inflammatory response
B	0008217	biological_process	regulation of blood pressure
B	0015670	biological_process	carbon dioxide transport
B	0015671	biological_process	oxygen transport
B	0019825	molecular_function	oxygen binding
B	0020037	molecular_function	heme binding
B	0030185	biological_process	nitric oxide transport
B	0030492	molecular_function	hemoglobin binding
B	0031720	molecular_function	haptoglobin binding
B	0031721	molecular_function	hemoglobin alpha binding
B	0031838	cellular_component	haptoglobin-hemoglobin complex
B	0042542	biological_process	response to hydrogen peroxide
B	0042744	biological_process	hydrogen peroxide catabolic process
B	0045429	biological_process	positive regulation of nitric oxide biosynthetic process
B	0046872	molecular_function	metal ion binding
B	0048821	biological_process	erythrocyte development
B	0070062	cellular_component	extracellular exosome
B	0070293	biological_process	renal absorption
B	0070527	biological_process	platelet aggregation
B	0071682	cellular_component	endocytic vesicle lumen
B	0072562	cellular_component	blood microparticle
B	0097746	biological_process	blood vessel diameter maintenance
B	0098869	biological_process	cellular oxidant detoxification
B	1904724	cellular_component	tertiary granule lumen
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0004601	molecular_function	peroxidase activity
C	0005344	molecular_function	oxygen carrier activity
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005829	cellular_component	cytosol
C	0005833	cellular_component	hemoglobin complex
C	0006954	biological_process	inflammatory response
C	0015670	biological_process	carbon dioxide transport
C	0015671	biological_process	oxygen transport
C	0016020	cellular_component	membrane
C	0019825	molecular_function	oxygen binding
C	0020037	molecular_function	heme binding
C	0030185	biological_process	nitric oxide transport
C	0031720	molecular_function	haptoglobin binding
C	0031838	cellular_component	haptoglobin-hemoglobin complex
C	0042542	biological_process	response to hydrogen peroxide
C	0042744	biological_process	hydrogen peroxide catabolic process
C	0046872	molecular_function	metal ion binding
C	0048821	biological_process	erythrocyte development
C	0070062	cellular_component	extracellular exosome
C	0071682	cellular_component	endocytic vesicle lumen
C	0072562	cellular_component	blood microparticle
C	0098869	biological_process	cellular oxidant detoxification
D	0004601	molecular_function	peroxidase activity
D	0005344	molecular_function	oxygen carrier activity
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005829	cellular_component	cytosol
D	0005833	cellular_component	hemoglobin complex
D	0006954	biological_process	inflammatory response
D	0008217	biological_process	regulation of blood pressure
D	0015670	biological_process	carbon dioxide transport
D	0015671	biological_process	oxygen transport
D	0019825	molecular_function	oxygen binding
D	0020037	molecular_function	heme binding
D	0030185	biological_process	nitric oxide transport
D	0030492	molecular_function	hemoglobin binding
D	0031720	molecular_function	haptoglobin binding
D	0031721	molecular_function	hemoglobin alpha binding
D	0031838	cellular_component	haptoglobin-hemoglobin complex
D	0042542	biological_process	response to hydrogen peroxide
D	0042744	biological_process	hydrogen peroxide catabolic process
D	0045429	biological_process	positive regulation of nitric oxide biosynthetic process
D	0046872	molecular_function	metal ion binding
D	0048821	biological_process	erythrocyte development
D	0070062	cellular_component	extracellular exosome
D	0070293	biological_process	renal absorption
D	0070527	biological_process	platelet aggregation
D	0071682	cellular_component	endocytic vesicle lumen
D	0072562	cellular_component	blood microparticle
D	0097746	biological_process	blood vessel diameter maintenance
D	0098869	biological_process	cellular oxidant detoxification
D	1904724	cellular_component	tertiary granule lumen
D	1904813	cellular_component	ficolin-1-rich granule lumen
P	0004601	molecular_function	peroxidase activity
P	0005344	molecular_function	oxygen carrier activity
P	0005506	molecular_function	iron ion binding
P	0005515	molecular_function	protein binding
P	0005576	cellular_component	extracellular region
P	0005615	cellular_component	extracellular space
P	0005829	cellular_component	cytosol
P	0005833	cellular_component	hemoglobin complex
P	0006954	biological_process	inflammatory response
P	0015670	biological_process	carbon dioxide transport
P	0015671	biological_process	oxygen transport
P	0016020	cellular_component	membrane
P	0019825	molecular_function	oxygen binding
P	0020037	molecular_function	heme binding
P	0030185	biological_process	nitric oxide transport
P	0031720	molecular_function	haptoglobin binding
P	0031838	cellular_component	haptoglobin-hemoglobin complex
P	0042542	biological_process	response to hydrogen peroxide
P	0042744	biological_process	hydrogen peroxide catabolic process
P	0046872	molecular_function	metal ion binding
P	0048821	biological_process	erythrocyte development
P	0070062	cellular_component	extracellular exosome
P	0071682	cellular_component	endocytic vesicle lumen
P	0072562	cellular_component	blood microparticle
P	0098869	biological_process	cellular oxidant detoxification
Q	0004601	molecular_function	peroxidase activity
Q	0005344	molecular_function	oxygen carrier activity
Q	0005515	molecular_function	protein binding
Q	0005576	cellular_component	extracellular region
Q	0005615	cellular_component	extracellular space
Q	0005829	cellular_component	cytosol
Q	0005833	cellular_component	hemoglobin complex
Q	0006954	biological_process	inflammatory response
Q	0008217	biological_process	regulation of blood pressure
Q	0015670	biological_process	carbon dioxide transport
Q	0015671	biological_process	oxygen transport
Q	0019825	molecular_function	oxygen binding
Q	0020037	molecular_function	heme binding
Q	0030185	biological_process	nitric oxide transport
Q	0030492	molecular_function	hemoglobin binding
Q	0031720	molecular_function	haptoglobin binding
Q	0031721	molecular_function	hemoglobin alpha binding
Q	0031838	cellular_component	haptoglobin-hemoglobin complex
Q	0042542	biological_process	response to hydrogen peroxide
Q	0042744	biological_process	hydrogen peroxide catabolic process
Q	0045429	biological_process	positive regulation of nitric oxide biosynthetic process
Q	0046872	molecular_function	metal ion binding
Q	0048821	biological_process	erythrocyte development
Q	0070062	cellular_component	extracellular exosome
Q	0070293	biological_process	renal absorption
Q	0070527	biological_process	platelet aggregation
Q	0071682	cellular_component	endocytic vesicle lumen
Q	0072562	cellular_component	blood microparticle
Q	0097746	biological_process	blood vessel diameter maintenance
Q	0098869	biological_process	cellular oxidant detoxification
Q	1904724	cellular_component	tertiary granule lumen
Q	1904813	cellular_component	ficolin-1-rich granule lumen
R	0004601	molecular_function	peroxidase activity
R	0005344	molecular_function	oxygen carrier activity
R	0005506	molecular_function	iron ion binding
R	0005515	molecular_function	protein binding
R	0005576	cellular_component	extracellular region
R	0005615	cellular_component	extracellular space
R	0005829	cellular_component	cytosol
R	0005833	cellular_component	hemoglobin complex
R	0006954	biological_process	inflammatory response
R	0015670	biological_process	carbon dioxide transport
R	0015671	biological_process	oxygen transport
R	0016020	cellular_component	membrane
R	0019825	molecular_function	oxygen binding
R	0020037	molecular_function	heme binding
R	0030185	biological_process	nitric oxide transport
R	0031720	molecular_function	haptoglobin binding
R	0031838	cellular_component	haptoglobin-hemoglobin complex
R	0042542	biological_process	response to hydrogen peroxide
R	0042744	biological_process	hydrogen peroxide catabolic process
R	0046872	molecular_function	metal ion binding
R	0048821	biological_process	erythrocyte development
R	0070062	cellular_component	extracellular exosome
R	0071682	cellular_component	endocytic vesicle lumen
R	0072562	cellular_component	blood microparticle
R	0098869	biological_process	cellular oxidant detoxification
S	0004601	molecular_function	peroxidase activity
S	0005344	molecular_function	oxygen carrier activity
S	0005515	molecular_function	protein binding
S	0005576	cellular_component	extracellular region
S	0005615	cellular_component	extracellular space
S	0005829	cellular_component	cytosol
S	0005833	cellular_component	hemoglobin complex
S	0006954	biological_process	inflammatory response
S	0008217	biological_process	regulation of blood pressure
S	0015670	biological_process	carbon dioxide transport
S	0015671	biological_process	oxygen transport
S	0019825	molecular_function	oxygen binding
S	0020037	molecular_function	heme binding
S	0030185	biological_process	nitric oxide transport
S	0030492	molecular_function	hemoglobin binding
S	0031720	molecular_function	haptoglobin binding
S	0031721	molecular_function	hemoglobin alpha binding
S	0031838	cellular_component	haptoglobin-hemoglobin complex
S	0042542	biological_process	response to hydrogen peroxide
S	0042744	biological_process	hydrogen peroxide catabolic process
S	0045429	biological_process	positive regulation of nitric oxide biosynthetic process
S	0046872	molecular_function	metal ion binding
S	0048821	biological_process	erythrocyte development
S	0070062	cellular_component	extracellular exosome
S	0070293	biological_process	renal absorption
S	0070527	biological_process	platelet aggregation
S	0071682	cellular_component	endocytic vesicle lumen
S	0072562	cellular_component	blood microparticle
S	0097746	biological_process	blood vessel diameter maintenance
S	0098869	biological_process	cellular oxidant detoxification
S	1904724	cellular_component	tertiary granule lumen
S	1904813	cellular_component	ficolin-1-rich granule lumen

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE HEM A 142

Chain	Residue
A	MET32
A	LEU91
A	VAL93
A	ASN97
A	PHE98
A	VAL132
A	LEU136
A	HOH167
A	TYR42
A	PHE43
A	HIS45
A	PHE46
A	HIS58
A	LYS61
A	LEU83
A	HIS87

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE HEM B 147

Chain	Residue
B	PHE41
B	PHE42
B	HIS63
B	HIS92
B	LEU96
B	VAL98
B	PHE103
B	LEU106
B	LEU141

---

site_id	AC3
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM C 142

Chain	Residue
C	TYR42
C	PHE43
C	HIS45
C	PHE46
C	HIS58
C	LYS61
C	LEU83
C	HIS87
C	LEU91
C	VAL93
C	ASN97
C	PHE98
C	LEU101
C	VAL132

---

site_id	AC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE HEM D 147

Chain	Residue
D	PHE41
D	PHE42
D	HIS63
D	VAL67
D	LEU91
D	HIS92
D	ASN102
D	PHE103
D	LEU106
D	LEU141
P	ALA53

---

site_id	AC5
Number of Residues	18
Details	BINDING SITE FOR RESIDUE HEM P 142

Chain	Residue
P	TYR42
P	PHE43
P	HIS45
P	PHE46
P	HIS58
P	LYS61
P	VAL62
P	ALA65
P	LEU83
P	HIS87
P	LEU91
P	VAL93
P	ASN97
P	PHE98
P	LEU101
P	VAL132
P	LEU136
P	HOH157

---

site_id	AC6
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM Q 147

Chain	Residue
C	ALA53
C	HOH149
Q	PHE41
Q	PHE42
Q	HIS63
Q	VAL67
Q	ALA70
Q	PHE71
Q	LEU88
Q	HIS92
Q	LEU96
Q	ASN102
Q	LEU106
Q	LEU141

---

site_id	AC7
Number of Residues	15
Details	BINDING SITE FOR RESIDUE HEM R 142

Chain	Residue
R	THR39
R	TYR42
R	PHE43
R	HIS45
R	PHE46
R	HIS58
R	LYS61
R	LEU86
R	HIS87
R	LEU91
R	VAL93
R	ASN97
R	PHE98
R	LEU101
R	HOH152

---

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE HEM S 147

Chain	Residue
S	HIS63
S	VAL67
S	HIS92
S	ASN102
S	PHE103
S	LEU106
S	LEU141
S	PHE41
S	PHE42

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	32
Details	Peptide: {"description":"Hemopressin","featureId":"PRO_0000455882","evidences":[{"source":"PubMed","id":"18077343","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1136
Details	Domain: {"description":"Globin","evidences":[{"source":"PROSITE-ProRule","id":"PRU00238","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00238","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"description":"proximal binding residue","evidences":[{"source":"PROSITE-ProRule","id":"PRU00238","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	132
Details	Site: {"description":"(Microbial infection) Cleavage; by N.americanus apr-2","evidences":[{"source":"PubMed","id":"12552433","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	32
Details	Site: {"description":"Not glycated","evidences":[{"source":"PubMed","id":"7358733","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	20
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	12
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P01942","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	16
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"P01942","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	16
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P01942","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	12
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P01942","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	16
Details	Glycosylation: {"description":"N-linked (Glc) (glycation) lysine; alternate","evidences":[{"source":"PubMed","id":"7358733","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	20
Details	Glycosylation: {"description":"N-linked (Glc) (glycation) lysine","evidences":[{"source":"PubMed","id":"7358733","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	36
Details	Peptide: {"description":"LVV-hemorphin-7","featureId":"PRO_0000296641"}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	24
Details	Peptide: {"description":"Spinorphin","featureId":"PRO_0000424226"}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	4
Details	Binding site: {"description":"distal binding residue"}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	4
Details	Binding site: {"description":"proximal binding residue"}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	4
Details	Modified residue: {"description":"N-pyruvate 2-iminyl-valine; in Hb A1b"}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"4531009","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	4
Details	Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"PubMed","id":"8637569","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9843411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"PubMed","id":"4531009","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (Glc) (glycation) valine; in Hb A1c","evidences":[{"source":"PubMed","id":"635569","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---