1KH1

Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000050	biological_process	urea cycle
A	0000053	biological_process	argininosuccinate metabolic process
A	0004055	molecular_function	argininosuccinate synthase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006526	biological_process	arginine biosynthetic process
A	0016874	molecular_function	ligase activity
B	0000050	biological_process	urea cycle
B	0000053	biological_process	argininosuccinate metabolic process
B	0004055	molecular_function	argininosuccinate synthase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006526	biological_process	arginine biosynthetic process
B	0016874	molecular_function	ligase activity
C	0000050	biological_process	urea cycle
C	0000053	biological_process	argininosuccinate metabolic process
C	0004055	molecular_function	argininosuccinate synthase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006526	biological_process	arginine biosynthetic process
C	0016874	molecular_function	ligase activity
D	0000050	biological_process	urea cycle
D	0000053	biological_process	argininosuccinate metabolic process
D	0004055	molecular_function	argininosuccinate synthase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006526	biological_process	arginine biosynthetic process
D	0016874	molecular_function	ligase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 510

Chain	Residue
A	SER8
A	GLY10
A	ASP12
A	THR13
A	HOH1457

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 B 1510

Chain	Residue
B	HOH1194
B	HOH1226
B	SER8
B	GLY10
B	ASP12
B	THR13

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 2510

Chain	Residue
C	SER8
C	GLY10
C	ASP12
C	THR13

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 3510

Chain	Residue
D	SER8
D	GLY10
D	ASP12
D	THR13

---

Functional Information from PROSITE/UniProt

site_id	PS00564
Number of Residues	9
Details	ARGININOSUCCIN_SYN_1 Argininosuccinate synthase signature 1. AYSGGLDTS

Chain	Residue	Details
A	ALA6-SER14

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site_id	PS00565
Number of Residues	12
Details	ARGININOSUCCIN_SYN_2 Argininosuccinate synthase signature 2. GaTgKGNDqvRF

Chain	Residue	Details
A	GLY114-PHE125

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00005, ECO:0000269|PubMed:11844799, ECO:0000269|PubMed:12684518

Chain	Residue	Details
A	ALA6
D	ALA6
D	ALA33
D	GLY114
A	ALA33
A	GLY114
B	ALA6
B	ALA33
B	GLY114
C	ALA6
C	ALA33
C	GLY114

---

site_id	SWS_FT_FI2
Number of Residues	40
Details	BINDING:

Chain	Residue	Details
A	TYR84
A	TYR270
B	TYR84
B	SER89
B	THR116
B	ASN120
B	ASP121
B	ARG124
B	SER173
B	SER182
B	GLU258
A	SER89
B	TYR270
C	TYR84
C	SER89
C	THR116
C	ASN120
C	ASP121
C	ARG124
C	SER173
C	SER182
C	GLU258
A	THR116
C	TYR270
D	TYR84
D	SER89
D	THR116
D	ASN120
D	ASP121
D	ARG124
D	SER173
D	SER182
D	GLU258
A	ASN120
D	TYR270
A	ASP121
A	ARG124
A	SER173
A	SER182
A	GLU258

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1kp2

Chain	Residue	Details
A	ASP121
A	ARG92

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1kp2

Chain	Residue	Details
B	ASP121
B	ARG92

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1kp2

Chain	Residue	Details
C	ASP121
C	ARG92

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1kp2

Chain	Residue	Details
D	ASP121
D	ARG92

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 316

Chain	Residue	Details
A	ASP12	steric role
A	ARG92	electrostatic stabiliser, hydrogen bond donor
A	ASP121	proton acceptor, proton donor
A	SER173	hydrogen bond donor, steric role

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 316

Chain	Residue	Details
B	ASP12	steric role
B	ARG92	electrostatic stabiliser, hydrogen bond donor
B	ASP121	proton acceptor, proton donor
B	SER173	hydrogen bond donor, steric role

---

site_id	MCSA3
Number of Residues	4
Details	M-CSA 316

Chain	Residue	Details
C	ASP12	steric role
C	ARG92	electrostatic stabiliser, hydrogen bond donor
C	ASP121	proton acceptor, proton donor
C	SER173	hydrogen bond donor, steric role

---

site_id	MCSA4
Number of Residues	4
Details	M-CSA 316

Chain	Residue	Details
D	ASP12	steric role
D	ARG92	electrostatic stabiliser, hydrogen bond donor
D	ASP121	proton acceptor, proton donor
D	SER173	hydrogen bond donor, steric role

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