1K70

The Structure of Escherichia coli Cytosine Deaminase bound to 4-Hydroxy-3,4-Dihydro-1H-Pyrimidin-2-one

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004131	molecular_function	cytosine deaminase activity
A	0005829	cellular_component	cytosol
A	0006209	biological_process	cytosine catabolic process
A	0008198	molecular_function	ferrous iron binding
A	0008270	molecular_function	zinc ion binding
A	0016787	molecular_function	hydrolase activity
A	0016810	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
A	0019858	biological_process	cytosine metabolic process
A	0035888	molecular_function	isoguanine deaminase activity
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FE A 502

Chain	Residue
A	HIS61
A	HIS63
A	HIS214
A	ASP313
A	HPY501

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site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE HPY A 501

Chain	Residue
A	HIS214
A	GLU217
A	HIS246
A	ASP313
A	ASP314
A	TRP319
A	FE502
A	HOH665
A	HIS63
A	LEU81
A	PHE154
A	GLN156

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:11812140, ECO:0000305|PubMed:21545144, ECO:0000305|PubMed:21604715

Chain	Residue	Details
A	ILE218

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site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:21545144, ECO:0000269|PubMed:21604715, ECO:0000269|Ref.14

Chain	Residue	Details
A	ILE62
A	LEU64
A	CYS215
A	ASP314

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site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11812140, ECO:0000269|PubMed:15381761, ECO:0000269|PubMed:21545144, ECO:0000269|PubMed:21604715

Chain	Residue	Details
A	GLU157

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site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11812140, ECO:0000269|PubMed:15381761, ECO:0000269|PubMed:21545144

Chain	Residue	Details
A	TYR320

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site_id	SWS_FT_FI5
Number of Residues	1
Details	SITE: Activates the nucleophilic water => ECO:0000305|PubMed:21545144, ECO:0000305|PubMed:21604715

Chain	Residue	Details
A	THR247

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 710

Chain	Residue	Details
A	ILE62	metal ligand
A	LEU64	metal ligand
A	GLU157	electrostatic stabiliser
A	CYS215	metal ligand
A	ILE218	proton acceptor, proton donor
A	ASP314	metal ligand

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