1JQ3

Crystal Structure of Spermidine Synthase in Complex with Transition State Analogue AdoDATO

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004766	molecular_function	spermidine synthase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006596	biological_process	polyamine biosynthetic process
A	0008295	biological_process	spermidine biosynthetic process
A	0010487	molecular_function	thermospermine synthase activity
A	0016740	molecular_function	transferase activity
A	0043918	molecular_function	cadaverine aminopropyltransferase activity
A	0043919	molecular_function	agmatine aminopropyltransferase activity
A	0050314	molecular_function	sym-norspermidine synthase activity
B	0003824	molecular_function	catalytic activity
B	0004766	molecular_function	spermidine synthase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006596	biological_process	polyamine biosynthetic process
B	0008295	biological_process	spermidine biosynthetic process
B	0010487	molecular_function	thermospermine synthase activity
B	0016740	molecular_function	transferase activity
B	0043918	molecular_function	cadaverine aminopropyltransferase activity
B	0043919	molecular_function	agmatine aminopropyltransferase activity
B	0050314	molecular_function	sym-norspermidine synthase activity
C	0003824	molecular_function	catalytic activity
C	0004766	molecular_function	spermidine synthase activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006596	biological_process	polyamine biosynthetic process
C	0008295	biological_process	spermidine biosynthetic process
C	0010487	molecular_function	thermospermine synthase activity
C	0016740	molecular_function	transferase activity
C	0043918	molecular_function	cadaverine aminopropyltransferase activity
C	0043919	molecular_function	agmatine aminopropyltransferase activity
C	0050314	molecular_function	sym-norspermidine synthase activity
D	0003824	molecular_function	catalytic activity
D	0004766	molecular_function	spermidine synthase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006596	biological_process	polyamine biosynthetic process
D	0008295	biological_process	spermidine biosynthetic process
D	0010487	molecular_function	thermospermine synthase activity
D	0016740	molecular_function	transferase activity
D	0043918	molecular_function	cadaverine aminopropyltransferase activity
D	0043919	molecular_function	agmatine aminopropyltransferase activity
D	0050314	molecular_function	sym-norspermidine synthase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	26
Details	BINDING SITE FOR RESIDUE AAT A 800

Chain	Residue
A	GLN46
A	CYS120
A	GLU121
A	VAL122
A	VAL126
A	ASN152
A	GLY153
A	ASP170
A	SER171
A	THR172
A	ASP173
A	LEU62
A	GLN178
A	GLY179
A	TYR239
A	TRP244
A	HOH802
A	HOH899
A	HOH906
A	THR66
A	MET67
A	TYR76
A	HIS77
A	GLY98
A	GLY99
A	ASP101

---

site_id	AC2
Number of Residues	26
Details	BINDING SITE FOR RESIDUE AAT C 801

Chain	Residue
C	GLN46
C	LEU62
C	THR66
C	MET67
C	THR68
C	TYR76
C	HIS77
C	GLY98
C	GLY99
C	GLY100
C	ASP101
C	CYS120
C	GLU121
C	VAL122
C	VAL126
C	ALA151
C	GLY153
C	ASP170
C	SER171
C	THR172
C	ASP173
C	LEU182
C	TYR239
C	TRP244
C	HOH803
C	HOH888

---

site_id	AC3
Number of Residues	25
Details	BINDING SITE FOR RESIDUE AAT D 802

Chain	Residue
D	GLN46
D	LEU62
D	MET67
D	TYR76
D	HIS77
D	GLY98
D	GLY99
D	ASP101
D	CYS120
D	GLU121
D	VAL122
D	VAL126
D	ASN152
D	GLY153
D	ASP170
D	SER171
D	THR172
D	ASP173
D	GLY177
D	GLN178
D	GLY179
D	TYR239
D	TRP244
D	HOH803
D	HOH885

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Functional Information from PROSITE/UniProt

site_id	PS01330
Number of Residues	14
Details	PABS_1 Polyamine biosynthesis (PABS) domain signature. VLIIGGGdGgtLrE

Chain	Residue	Details
A	VAL94-GLU107

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00198, ECO:0000305|PubMed:11731804

Chain	Residue	Details
A	ASP170
B	ASP170
C	ASP170
D	ASP170

---

site_id	SWS_FT_FI2
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269|PubMed:11731804

Chain	Residue	Details
A	GLN46
B	GLU121
B	ASN152
B	ASP170
C	GLN46
C	HIS77
C	ASP101
C	GLU121
C	ASN152
C	ASP170
D	GLN46
A	HIS77
D	HIS77
D	ASP101
D	GLU121
D	ASN152
D	ASP170
A	ASP101
A	GLU121
A	ASN152
A	ASP170
B	GLN46
B	HIS77
B	ASP101

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