1J51
CRYSTAL STRUCTURE OF CYTOCHROME P450CAM MUTANT (F87W/Y96F/V247L/C334A) WITH 1,3,5-TRICHLOROBENZENE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004497 | molecular_function | monooxygenase activity |
| A | 0005506 | molecular_function | iron ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0016705 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| A | 0018683 | molecular_function | camphor 5-monooxygenase activity |
| A | 0019383 | biological_process | (+)-camphor catabolic process |
| A | 0020037 | molecular_function | heme binding |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0004497 | molecular_function | monooxygenase activity |
| B | 0005506 | molecular_function | iron ion binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0016705 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| B | 0018683 | molecular_function | camphor 5-monooxygenase activity |
| B | 0019383 | biological_process | (+)-camphor catabolic process |
| B | 0020037 | molecular_function | heme binding |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0004497 | molecular_function | monooxygenase activity |
| C | 0005506 | molecular_function | iron ion binding |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0016705 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| C | 0018683 | molecular_function | camphor 5-monooxygenase activity |
| C | 0019383 | biological_process | (+)-camphor catabolic process |
| C | 0020037 | molecular_function | heme binding |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0004497 | molecular_function | monooxygenase activity |
| D | 0005506 | molecular_function | iron ion binding |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0016705 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| D | 0018683 | molecular_function | camphor 5-monooxygenase activity |
| D | 0019383 | biological_process | (+)-camphor catabolic process |
| D | 0020037 | molecular_function | heme binding |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE K A 1418 |
| Chain | Residue |
| A | GLU84 |
| A | GLY93 |
| A | GLU94 |
| A | PHE96 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE K B 2418 |
| Chain | Residue |
| B | GLU84 |
| B | GLY93 |
| B | GLU94 |
| B | PHE96 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE K C 3418 |
| Chain | Residue |
| C | GLY93 |
| C | GLU94 |
| C | PHE96 |
| C | GLU84 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE K D 4418 |
| Chain | Residue |
| D | GLU84 |
| D | GLY93 |
| D | GLU94 |
| D | PHE96 |
| D | HOH4475 |
| site_id | AC5 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE HEM A 417 |
| Chain | Residue |
| A | PRO100 |
| A | THR101 |
| A | GLN108 |
| A | ARG112 |
| A | LEU244 |
| A | LEU245 |
| A | GLY248 |
| A | GLY249 |
| A | THR252 |
| A | ASP297 |
| A | ARG299 |
| A | GLN322 |
| A | THR349 |
| A | PHE350 |
| A | GLY351 |
| A | HIS355 |
| A | CYS357 |
| A | GLY359 |
| A | ALA363 |
| A | TCZ1450 |
| A | HOH1452 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE TCZ A 1450 |
| Chain | Residue |
| A | TRP87 |
| A | LEU244 |
| A | GLY248 |
| A | THR252 |
| A | VAL295 |
| A | ASP297 |
| A | HEM417 |
| site_id | AC7 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE HEM B 417 |
| Chain | Residue |
| B | PRO100 |
| B | THR101 |
| B | GLN108 |
| B | ARG112 |
| B | LEU245 |
| B | GLY248 |
| B | THR252 |
| B | VAL253 |
| B | ASP297 |
| B | ARG299 |
| B | GLN322 |
| B | THR349 |
| B | PHE350 |
| B | GLY351 |
| B | HIS355 |
| B | LEU356 |
| B | CYS357 |
| B | ALA363 |
| B | TCZ2450 |
| B | HOH2480 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE TCZ B 2450 |
| Chain | Residue |
| B | TRP87 |
| B | PHE96 |
| B | LEU244 |
| B | LEU247 |
| B | VAL295 |
| B | HEM417 |
| site_id | AC9 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE HEM C 417 |
| Chain | Residue |
| C | PRO100 |
| C | THR101 |
| C | GLN108 |
| C | ARG112 |
| C | LEU244 |
| C | LEU245 |
| C | GLY248 |
| C | GLY249 |
| C | THR252 |
| C | VAL295 |
| C | ASP297 |
| C | ARG299 |
| C | GLN322 |
| C | THR349 |
| C | PHE350 |
| C | GLY351 |
| C | HIS355 |
| C | CYS357 |
| C | LEU358 |
| C | GLY359 |
| C | TCZ3450 |
| C | HOH3462 |
| site_id | BC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE TCZ C 3450 |
| Chain | Residue |
| C | HEM417 |
| C | TRP87 |
| C | LEU244 |
| C | GLY248 |
| C | THR252 |
| C | VAL295 |
| C | ASP297 |
| site_id | BC2 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE HEM D 417 |
| Chain | Residue |
| D | PRO100 |
| D | THR101 |
| D | GLN108 |
| D | ARG112 |
| D | LEU244 |
| D | LEU245 |
| D | GLY248 |
| D | THR252 |
| D | ASP297 |
| D | ARG299 |
| D | GLN322 |
| D | THR349 |
| D | PHE350 |
| D | GLY351 |
| D | HIS355 |
| D | CYS357 |
| D | ALA363 |
| D | HOH4464 |
| D | HOH4502 |
Functional Information from PROSITE/UniProt
| site_id | PS00086 |
| Number of Residues | 10 |
| Details | CYTOCHROME_P450 Cytochrome P450 cysteine heme-iron ligand signature. FGhGSHLCLG |
| Chain | Residue | Details |
| A | PHE350-GLY359 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | BINDING: axial binding residue |
| Chain | Residue | Details |
| A | LEU358 | |
| B | LEU358 | |
| C | LEU358 | |
| D | LEU358 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1akd |
| Chain | Residue | Details |
| A | ASP251 | |
| A | THR252 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1akd |
| Chain | Residue | Details |
| B | ASP251 | |
| B | THR252 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1akd |
| Chain | Residue | Details |
| C | ASP251 | |
| C | THR252 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1akd |
| Chain | Residue | Details |
| D | ASP251 | |
| D | THR252 |
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 133 |
| Chain | Residue | Details |
| A | PRO187 | hydrogen bond donor, proton acceptor, proton donor, proton relay |
| A | THR252 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| A | VAL253 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| A | LEU358 | electrostatic stabiliser, hydrogen bond acceptor, metal ligand |
| A | GLY359 | electrostatic stabiliser, hydrogen bond donor |
| A | GLN360 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 133 |
| Chain | Residue | Details |
| B | PRO187 | hydrogen bond donor, proton acceptor, proton donor, proton relay |
| B | THR252 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| B | VAL253 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| B | LEU358 | electrostatic stabiliser, hydrogen bond acceptor, metal ligand |
| B | GLY359 | electrostatic stabiliser, hydrogen bond donor |
| B | GLN360 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA3 |
| Number of Residues | 6 |
| Details | M-CSA 133 |
| Chain | Residue | Details |
| C | PRO187 | hydrogen bond donor, proton acceptor, proton donor, proton relay |
| C | THR252 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| C | VAL253 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| C | LEU358 | electrostatic stabiliser, hydrogen bond acceptor, metal ligand |
| C | GLY359 | electrostatic stabiliser, hydrogen bond donor |
| C | GLN360 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA4 |
| Number of Residues | 6 |
| Details | M-CSA 133 |
| Chain | Residue | Details |
| D | PRO187 | hydrogen bond donor, proton acceptor, proton donor, proton relay |
| D | THR252 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| D | VAL253 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| D | LEU358 | electrostatic stabiliser, hydrogen bond acceptor, metal ligand |
| D | GLY359 | electrostatic stabiliser, hydrogen bond donor |
| D | GLN360 | electrostatic stabiliser, hydrogen bond donor |
