Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1J0X

Crystal structure of the rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000226	biological_process	microtubule cytoskeleton organization
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0006006	biological_process	glucose metabolic process
O	0006096	biological_process	glycolytic process
O	0006417	biological_process	regulation of translation
O	0006915	biological_process	apoptotic process
O	0008017	molecular_function	microtubule binding
O	0015630	cellular_component	microtubule cytoskeleton
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0016740	molecular_function	transferase activity
O	0032481	biological_process	positive regulation of type I interferon production
O	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
O	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
O	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
O	0045087	biological_process	innate immune response
O	0050661	molecular_function	NADP binding
O	0050821	biological_process	protein stabilization
O	0051287	molecular_function	NAD binding
O	0051402	biological_process	neuron apoptotic process
O	0097452	cellular_component	GAIT complex
P	0000226	biological_process	microtubule cytoskeleton organization
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005856	cellular_component	cytoskeleton
P	0006006	biological_process	glucose metabolic process
P	0006096	biological_process	glycolytic process
P	0006417	biological_process	regulation of translation
P	0006915	biological_process	apoptotic process
P	0008017	molecular_function	microtubule binding
P	0015630	cellular_component	microtubule cytoskeleton
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0016740	molecular_function	transferase activity
P	0032481	biological_process	positive regulation of type I interferon production
P	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
P	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
P	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
P	0045087	biological_process	innate immune response
P	0050661	molecular_function	NADP binding
P	0050821	biological_process	protein stabilization
P	0051287	molecular_function	NAD binding
P	0051402	biological_process	neuron apoptotic process
P	0097452	cellular_component	GAIT complex
Q	0000226	biological_process	microtubule cytoskeleton organization
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005856	cellular_component	cytoskeleton
Q	0006006	biological_process	glucose metabolic process
Q	0006096	biological_process	glycolytic process
Q	0006417	biological_process	regulation of translation
Q	0006915	biological_process	apoptotic process
Q	0008017	molecular_function	microtubule binding
Q	0015630	cellular_component	microtubule cytoskeleton
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0016740	molecular_function	transferase activity
Q	0032481	biological_process	positive regulation of type I interferon production
Q	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
Q	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
Q	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
Q	0045087	biological_process	innate immune response
Q	0050661	molecular_function	NADP binding
Q	0050821	biological_process	protein stabilization
Q	0051287	molecular_function	NAD binding
Q	0051402	biological_process	neuron apoptotic process
Q	0097452	cellular_component	GAIT complex
R	0000226	biological_process	microtubule cytoskeleton organization
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005856	cellular_component	cytoskeleton
R	0006006	biological_process	glucose metabolic process
R	0006096	biological_process	glycolytic process
R	0006417	biological_process	regulation of translation
R	0006915	biological_process	apoptotic process
R	0008017	molecular_function	microtubule binding
R	0015630	cellular_component	microtubule cytoskeleton
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0016740	molecular_function	transferase activity
R	0032481	biological_process	positive regulation of type I interferon production
R	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
R	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
R	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
R	0045087	biological_process	innate immune response
R	0050661	molecular_function	NADP binding
R	0050821	biological_process	protein stabilization
R	0051287	molecular_function	NAD binding
R	0051402	biological_process	neuron apoptotic process
R	0097452	cellular_component	GAIT complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NAD P 1336

Chain	Residue
P	ASN6
P	ARG77
P	SER95
P	THR96
P	GLY97
P	PHE99
P	SER119
P	ALA120
P	CSX149
P	ASN313
P	TYR317
P	PHE8
P	HOH1375
P	GLY9
P	ARG10
P	ILE11
P	ASP32
P	PRO33
P	PHE34
P	ILE35

site_id	AC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE NAD R 2336

Chain	Residue
O	HOH363
R	ASN6
R	PHE8
R	GLY9
R	ARG10
R	ILE11
R	ASP32
R	PRO33
R	PHE34
R	ILE35
R	ARG77
R	SER95
R	THR96
R	GLY97
R	PHE99
R	SER119
R	ALA120
R	CSX149
R	ASN313
R	TYR317
R	HOH2428

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA147-LEU154

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
O	THR150
P	THR150
Q	THR150
R	THR150

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:14646080

Chain	Residue	Details
O	ILE11
Q	PRO33
Q	ASP78
Q	GLU314
R	ILE11
R	PRO33
R	ASP78
R	GLU314
O	PRO33
O	ASP78
O	GLU314
P	ILE11
P	PRO33
P	ASP78
P	GLU314
Q	ILE11

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	ALA120
P	ALA120
Q	ALA120
R	ALA120

site_id	SWS_FT_FI4
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P22513

Chain	Residue	Details
O	CSX149
Q	ALA180
Q	GLY209
Q	VAL232
R	CSX149
R	ALA180
R	GLY209
R	VAL232
O	ALA180
O	GLY209
O	VAL232
P	CSX149
P	ALA180
P	GLY209
P	VAL232
Q	CSX149

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Activates thiol group during catalysis => ECO:0000305\|PubMed:14646080

Chain	Residue	Details
O	ALA177
P	ALA177
Q	ALA177
R	ALA177

site_id	SWS_FT_FI6
Number of Residues	20
Details	MOD_RES: N6,N6-dimethyllysine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	VAL3
P	GLU332
Q	VAL3
Q	LEU64
Q	VAL258
Q	GLN261
Q	GLU332
R	VAL3
R	LEU64
R	VAL258
R	GLN261
O	LEU64
R	GLU332
O	VAL258
O	GLN261
O	GLU332
P	VAL3
P	LEU64
P	VAL258
P	GLN261

site_id	SWS_FT_FI7
Number of Residues	28
Details	MOD_RES: Deamidated asparagine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	GLY7
P	GLY68
P	ALA147
P	CYS153
P	GLY223
P	GLU314
Q	GLY7
Q	GLY62
Q	GLY68
Q	ALA147
Q	CYS153
O	GLY62
Q	GLY223
Q	GLU314
R	GLY7
R	GLY62
R	GLY68
R	ALA147
R	CYS153
R	GLY223
R	GLU314
O	GLY68
O	ALA147
O	CYS153
O	GLY223
O	GLU314
P	GLY7
P	GLY62

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	MET40
P	MET40
Q	MET40
R	MET40

site_id	SWS_FT_FI9
Number of Residues	12
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	ALA59
R	ALA59
R	VAL217
R	TYR252
O	VAL217
O	TYR252
P	ALA59
P	VAL217
P	TYR252
Q	ALA59
Q	VAL217
Q	TYR252

site_id	SWS_FT_FI10
Number of Residues	32
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	ILE73
P	THR151
P	VAL175
P	ALA180
P	GLN182
P	GLY209
P	GLY227
P	PRO235
Q	ILE73
Q	THR151
Q	VAL175
O	THR151
Q	ALA180
Q	GLN182
Q	GLY209
Q	GLY227
Q	PRO235
R	ILE73
R	THR151
R	VAL175
R	ALA180
R	GLN182
O	VAL175
R	GLY209
R	GLY227
R	PRO235
O	ALA180
O	GLN182
O	GLY209
O	GLY227
O	PRO235
P	ILE73

site_id	SWS_FT_FI11
Number of Residues	24
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	ALA120
P	VAL239
P	TRP310
P	LYS331
Q	ALA120
Q	ASN146
Q	CSX149
Q	VAL239
Q	TRP310
Q	LYS331
R	ALA120
O	ASN146
R	ASN146
R	CSX149
R	VAL239
R	TRP310
R	LYS331
O	CSX149
O	VAL239
O	TRP310
O	LYS331
P	ALA120
P	ASN146
P	CSX149

site_id	SWS_FT_FI12
Number of Residues	4
Details	MOD_RES: S-nitrosocysteine; in reversibly inhibited form => ECO:0000250\|UniProtKB:P04797

Chain	Residue	Details
O	THR150
P	THR150
Q	THR150
R	THR150

site_id	SWS_FT_FI13
Number of Residues	8
Details	MOD_RES: N6-malonyllysine; alternate => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	LEU192
O	ALA213
P	LEU192
P	ALA213
Q	LEU192
Q	ALA213
R	LEU192
R	ALA213

site_id	SWS_FT_FI14
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	LEU225
P	LEU225
Q	LEU225
R	LEU225

site_id	SWS_FT_FI15
Number of Residues	4
Details	MOD_RES: S-nitrosocysteine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	ARG245
P	ARG245
Q	ARG245
R	ARG245

site_id	SWS_FT_FI16
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
O	THR184
P	THR184
Q	THR184
R	THR184

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
O	HIS176

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
P	HIS176

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
Q	HIS176

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
R	HIS176

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)