1J0X

Crystal structure of the rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000226	biological_process	microtubule cytoskeleton organization
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0006006	biological_process	glucose metabolic process
O	0006096	biological_process	glycolytic process
O	0008017	molecular_function	microtubule binding
O	0015630	cellular_component	microtubule cytoskeleton
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0032481	biological_process	positive regulation of type I interferon production
O	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
O	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
O	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
O	0050661	molecular_function	NADP binding
O	0050821	biological_process	protein stabilization
O	0051287	molecular_function	NAD binding
O	0051402	biological_process	neuron apoptotic process
O	0097452	cellular_component	GAIT complex
P	0000226	biological_process	microtubule cytoskeleton organization
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005856	cellular_component	cytoskeleton
P	0006006	biological_process	glucose metabolic process
P	0006096	biological_process	glycolytic process
P	0008017	molecular_function	microtubule binding
P	0015630	cellular_component	microtubule cytoskeleton
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0032481	biological_process	positive regulation of type I interferon production
P	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
P	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
P	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
P	0050661	molecular_function	NADP binding
P	0050821	biological_process	protein stabilization
P	0051287	molecular_function	NAD binding
P	0051402	biological_process	neuron apoptotic process
P	0097452	cellular_component	GAIT complex
Q	0000226	biological_process	microtubule cytoskeleton organization
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005856	cellular_component	cytoskeleton
Q	0006006	biological_process	glucose metabolic process
Q	0006096	biological_process	glycolytic process
Q	0008017	molecular_function	microtubule binding
Q	0015630	cellular_component	microtubule cytoskeleton
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0032481	biological_process	positive regulation of type I interferon production
Q	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
Q	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
Q	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
Q	0050661	molecular_function	NADP binding
Q	0050821	biological_process	protein stabilization
Q	0051287	molecular_function	NAD binding
Q	0051402	biological_process	neuron apoptotic process
Q	0097452	cellular_component	GAIT complex
R	0000226	biological_process	microtubule cytoskeleton organization
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005856	cellular_component	cytoskeleton
R	0006006	biological_process	glucose metabolic process
R	0006096	biological_process	glycolytic process
R	0008017	molecular_function	microtubule binding
R	0015630	cellular_component	microtubule cytoskeleton
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0032481	biological_process	positive regulation of type I interferon production
R	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
R	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
R	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
R	0050661	molecular_function	NADP binding
R	0050821	biological_process	protein stabilization
R	0051287	molecular_function	NAD binding
R	0051402	biological_process	neuron apoptotic process
R	0097452	cellular_component	GAIT complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NAD P 1336

Chain	Residue
P	ASN6
P	ARG77
P	SER95
P	THR96
P	GLY97
P	PHE99
P	SER119
P	ALA120
P	CSX149
P	ASN313
P	TYR317
P	PHE8
P	HOH1375
P	GLY9
P	ARG10
P	ILE11
P	ASP32
P	PRO33
P	PHE34
P	ILE35

---

site_id	AC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE NAD R 2336

Chain	Residue
O	HOH363
R	ASN6
R	PHE8
R	GLY9
R	ARG10
R	ILE11
R	ASP32
R	PRO33
R	PHE34
R	ILE35
R	ARG77
R	SER95
R	THR96
R	GLY97
R	PHE99
R	SER119
R	ALA120
R	CSX149
R	ASN313
R	TYR317
R	HOH2428

---

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA147-LEU154

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	Motif: {"description":"[IL]-x-C-x-x-[DE] motif","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Nucleophile"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"14646080","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P22513","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Site: {"description":"Activates thiol group during catalysis","evidences":[{"source":"PubMed","id":"14646080","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	16
Details	Modified residue: {"description":"N6,N6-dimethyllysine","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	28
Details	Modified residue: {"description":"Deamidated asparagine","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	12
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	32
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	20
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	Modified residue: {"description":"S-nitrosocysteine; in reversibly inhibited form","evidences":[{"source":"UniProtKB","id":"P04797","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	8
Details	Modified residue: {"description":"N6-malonyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	4
Details	Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"UniProtKB","id":"P04406","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
O	HIS176

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
P	HIS176

---

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
Q	HIS176

---

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
R	HIS176

---