1IZJ

Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme f313a

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0008152	biological_process	metabolic process
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0031216	molecular_function	neopullulanase activity
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 1001

Chain	Residue
A	ALA2
A	ASP4
A	ASN6
A	ASP42
A	ASP96
A	HOH1082

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 1002

Chain	Residue
A	SER283
A	GLU288
A	ASP276
A	ASN279
A	PHE281

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 1003

Chain	Residue
A	ASN145
A	ASP147
A	ASN150
A	ASP151
A	GLY187
A	ASP189
A	HOH1019

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P38940

Chain	Residue	Details
A	ASP356

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000250|UniProtKB:P38940

Chain	Residue	Details
A	GLU396

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269|PubMed:12051850, ECO:0007744|PDB:1JI1

Chain	Residue	Details
A	ALA2
A	GLY187
A	ASP189
A	ASP276
A	ASN280
A	PHE281
A	SER283
A	GLU288
A	ASP4
A	ASN6
A	ASP42
A	ASP96
A	ASN145
A	ASP147
A	ASN150
A	ASP151

---

site_id	SWS_FT_FI4
Number of Residues	5
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P38940

Chain	Residue	Details
A	HIS267
A	ARG354
A	HIS471
A	ASP516
A	ARG520

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000250

Chain	Residue	Details
A	ASP472

---