Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1IRU

Crystal Structure of the mammalian 20S proteasome at 2.75 A resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0005839	cellular_component	proteasome core complex
1	0051603	biological_process	proteolysis involved in protein catabolic process
2	0005839	cellular_component	proteasome core complex
2	0051603	biological_process	proteolysis involved in protein catabolic process
A	0000502	cellular_component	proteasome complex
A	0000932	cellular_component	P-body
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005839	cellular_component	proteasome core complex
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
B	0000502	cellular_component	proteasome complex
B	0000932	cellular_component	P-body
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0051603	biological_process	proteolysis involved in protein catabolic process
C	0000502	cellular_component	proteasome complex
C	0000932	cellular_component	P-body
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
D	0000502	cellular_component	proteasome complex
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0042802	molecular_function	identical protein binding
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
E	0000502	cellular_component	proteasome complex
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
F	0000502	cellular_component	proteasome complex
F	0001530	molecular_function	lipopolysaccharide binding
F	0002376	biological_process	immune system process
F	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005813	cellular_component	centrosome
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0051603	biological_process	proteolysis involved in protein catabolic process
G	0000502	cellular_component	proteasome complex
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005839	cellular_component	proteasome core complex
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0045202	cellular_component	synapse
G	0051603	biological_process	proteolysis involved in protein catabolic process
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005839	cellular_component	proteasome core complex
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0004298	molecular_function	threonine-type endopeptidase activity
I	0005839	cellular_component	proteasome core complex
I	0051603	biological_process	proteolysis involved in protein catabolic process
J	0000502	cellular_component	proteasome complex
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
K	0000502	cellular_component	proteasome complex
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0010498	biological_process	proteasomal protein catabolic process
K	0019774	cellular_component	proteasome core complex, beta-subunit complex
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0051603	biological_process	proteolysis involved in protein catabolic process
L	0004298	molecular_function	threonine-type endopeptidase activity
L	0005839	cellular_component	proteasome core complex
L	0051603	biological_process	proteolysis involved in protein catabolic process
M	0005839	cellular_component	proteasome core complex
M	0051603	biological_process	proteolysis involved in protein catabolic process
N	0005839	cellular_component	proteasome core complex
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0000502	cellular_component	proteasome complex
O	0000932	cellular_component	P-body
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005839	cellular_component	proteasome core complex
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0019773	cellular_component	proteasome core complex, alpha-subunit complex
O	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0051603	biological_process	proteolysis involved in protein catabolic process
P	0000502	cellular_component	proteasome complex
P	0000932	cellular_component	P-body
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005839	cellular_component	proteasome core complex
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0019773	cellular_component	proteasome core complex, alpha-subunit complex
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0000502	cellular_component	proteasome complex
Q	0000932	cellular_component	P-body
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005839	cellular_component	proteasome core complex
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0051603	biological_process	proteolysis involved in protein catabolic process
R	0000502	cellular_component	proteasome complex
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005839	cellular_component	proteasome core complex
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0019773	cellular_component	proteasome core complex, alpha-subunit complex
R	0042802	molecular_function	identical protein binding
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0051603	biological_process	proteolysis involved in protein catabolic process
S	0000502	cellular_component	proteasome complex
S	0005634	cellular_component	nucleus
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0005839	cellular_component	proteasome core complex
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0019773	cellular_component	proteasome core complex, alpha-subunit complex
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051603	biological_process	proteolysis involved in protein catabolic process
T	0000502	cellular_component	proteasome complex
T	0001530	molecular_function	lipopolysaccharide binding
T	0002376	biological_process	immune system process
T	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005813	cellular_component	centrosome
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0051603	biological_process	proteolysis involved in protein catabolic process
U	0000502	cellular_component	proteasome complex
U	0005634	cellular_component	nucleus
U	0005737	cellular_component	cytoplasm
U	0005839	cellular_component	proteasome core complex
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0045202	cellular_component	synapse
U	0051603	biological_process	proteolysis involved in protein catabolic process
V	0004298	molecular_function	threonine-type endopeptidase activity
V	0005839	cellular_component	proteasome core complex
V	0051603	biological_process	proteolysis involved in protein catabolic process
W	0004298	molecular_function	threonine-type endopeptidase activity
W	0005839	cellular_component	proteasome core complex
W	0051603	biological_process	proteolysis involved in protein catabolic process
X	0000502	cellular_component	proteasome complex
X	0005634	cellular_component	nucleus
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005839	cellular_component	proteasome core complex
X	0019774	cellular_component	proteasome core complex, beta-subunit complex
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0051603	biological_process	proteolysis involved in protein catabolic process
Y	0000502	cellular_component	proteasome complex
Y	0005634	cellular_component	nucleus
Y	0005654	cellular_component	nucleoplasm
Y	0005737	cellular_component	cytoplasm
Y	0005829	cellular_component	cytosol
Y	0005839	cellular_component	proteasome core complex
Y	0010498	biological_process	proteasomal protein catabolic process
Y	0019774	cellular_component	proteasome core complex, beta-subunit complex
Y	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Y	0051603	biological_process	proteolysis involved in protein catabolic process
Z	0004298	molecular_function	threonine-type endopeptidase activity
Z	0005839	cellular_component	proteasome core complex
Z	0051603	biological_process	proteolysis involved in protein catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 301

Chain	Residue
A	THR14
A	TYR125
A	ASN128
A	ALA129
A	MET131

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG J 304

Chain	Residue
J	VAL175
J	ASP178
J	SER181
J	ASP205

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG I 306

Chain	Residue
1	ASP213
I	ILE163
I	PHE164
I	ASP166
I	SER169

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG X 307

Chain	Residue
L	ARG19
L	THR164
L	ASP167
L	ALA168
L	SER170
X	ASP205

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG D 309

Chain	Residue
D	ASN159
D	ALA160

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG M 310

Chain	Residue
M	SER40
M	PRO41

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG G 311

Chain	Residue
G	VAL45
G	VAL46
G	SER214
G	TRP215
G	VAL216

site_id	AC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG K 313

Chain	Residue
K	CYS91

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG C 314

Chain	Residue
C	GLY158
C	TRP159
D	GLN54

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG J 315

Chain	Residue
J	GLN65
K	ASN82
K	ARG85

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 316

Chain	Residue
B	ALA32
B	THR47
B	LYS63
B	TYR75
B	SER76

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG G 317

Chain	Residue
A	ALA85
G	HIS120
G	THR123
G	SER154

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG M 318

Chain	Residue
M	ARG1
M	PHE2
N	GLN2

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG G 319

Chain	Residue
G	LEU179
G	GLU192
G	LYS195
G	ILE196

site_id	BC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG J 320

Chain	Residue
J	ASP47
J	ARG48
J	ILE195

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG O 401

Chain	Residue
O	THR14
O	TYR125
O	ASN128
O	ALA129
O	MET131

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG X 404

Chain	Residue
X	VAL175
X	ASP178
X	ALA179
X	SER181
X	ASP205

site_id	BC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG W 406

Chain	Residue
M	ASP213
W	ILE163
W	PHE164
W	ASP166
W	LEU167
W	SER169

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG J 407

Chain	Residue
J	ASP205
Z	ARG19
Z	THR164
Z	ASP167
Z	ALA168
Z	SER170

site_id	CC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG R 409

Chain	Residue
R	ASN159
R	ALA160

site_id	CC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG 1 410

Chain	Residue
1	SER40
1	PRO41

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG U 411

Chain	Residue
U	VAL45
U	VAL46
U	SER214
U	TRP215
U	VAL216

site_id	CC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG Y 413

Chain	Residue
Y	CYS91

site_id	CC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG Q 414

Chain	Residue
Q	GLY158
Q	TRP159
R	GLN54

site_id	CC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG X 415

Chain	Residue
X	GLN65
Y	ASN82
Y	ARG85

site_id	CC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG P 416

Chain	Residue
P	ALA32
P	THR47
P	LYS63
P	TYR75
P	SER76

site_id	CC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG U 417

Chain	Residue
U	HIS120
U	THR123
U	SER154
O	ALA85

site_id	DC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG 1 418

Chain	Residue
1	ARG1
1	PHE2
2	GLN2

site_id	DC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG U 419

Chain	Residue
U	GLU192
U	LYS195
U	ILE196

site_id	DC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG X 420

Chain	Residue
X	ASP47
X	ARG48
X	ILE195

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YsfslTtFSPsGKlvQIEYAlaA

Chain	Residue	Details
B	TYR5-ALA27
D	TYR3-ALA25
C	TYR5-ALA27
A	PHE9-ALA31
E	TYR8-ALA30
F	TYR6-ALA28
G	TYR7-ALA29

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LAIaGedFAIVASDtrlsegfsihtrdsp.KcykltdktvigcsGfhGD

Chain	Residue	Details
1	LEU13-ASP60
J	MET12-ASP59
K	ILE5-ASP52
L	LEU4-ASP51
H	MET4-ASP51
C	LEU35-ASP82
2	LEU12-ASP59
I	ALA4-ASP51

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000250\|UniProtKB:P28074

Chain	Residue	Details
L	THR1
Z	THR1
C	SER173
Q	SER13
Q	SER75
Q	SER173

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
L	ALA49
Z	ALA49
G	LYS229
U	LYS56
U	LYS205
U	LYS229

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P25788

Chain	Residue	Details
G	SER242
G	SER249
U	SER242
U	SER249
P	SER13
P	SER15

site_id	SWS_FT_FI4
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine; alternate => ECO:0000250

Chain	Residue	Details
F	SER110
T	SER110
P	TYR23
P	TYR75

site_id	SWS_FT_FI5
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250\|UniProtKB:P25786

Chain	Residue	Details
F	LYS115
B	LYS170
F	LYS208
T	LYS115
T	LYS208

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:P17220

Chain	Residue	Details
B	TYR120
P	TYR120

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
L	GLY47
L	LYS33
L	THR1
L	SER130

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
Z	GLY47
Z	LYS33
Z	THR1
Z	SER130

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
E	GLY80
E	SER172
E	SER35
E	LYS66

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
S	GLY80
S	SER172
S	SER35
S	LYS66

site_id	CSA5
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
I	GLY47
I	LYS33
I	THR1
I	SER129

site_id	CSA6
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
W	GLY47
W	LYS33
W	THR1
W	SER129

site_id	CSA7
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
H	GLY47
H	LYS33
H	THR1
H	SER130

site_id	CSA8
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
V	GLY47
V	LYS33
V	THR1
V	SER130

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)