1IH8
NH3-dependent NAD+ Synthetase from Bacillus subtilis Complexed with AMP-CPP and Mg2+ ions.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003952 | molecular_function | NAD+ synthase (glutamine-hydrolyzing) activity |
| A | 0004359 | molecular_function | glutaminase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0008795 | molecular_function | NAD+ synthase activity |
| A | 0009435 | biological_process | NAD biosynthetic process |
| A | 0016874 | molecular_function | ligase activity |
| A | 0030435 | biological_process | sporulation resulting in formation of a cellular spore |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003952 | molecular_function | NAD+ synthase (glutamine-hydrolyzing) activity |
| B | 0004359 | molecular_function | glutaminase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0008795 | molecular_function | NAD+ synthase activity |
| B | 0009435 | biological_process | NAD biosynthetic process |
| B | 0016874 | molecular_function | ligase activity |
| B | 0030435 | biological_process | sporulation resulting in formation of a cellular spore |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 4002 |
| Chain | Residue |
| A | THR208 |
| A | HOH2385 |
| A | HOH2386 |
| A | APC3001 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 4003 |
| Chain | Residue |
| A | APC3001 |
| A | GLU162 |
| A | HOH2060 |
| A | HOH2248 |
| A | HOH2387 |
| A | HOH2388 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 4004 |
| Chain | Residue |
| B | ASP1124 |
| B | HOH2280 |
| B | HOH2367 |
| B | HOH2480 |
| B | HOH2481 |
| B | HOH2482 |
| site_id | AC4 |
| Number of Residues | 30 |
| Details | BINDING SITE FOR RESIDUE APC A 3001 |
| Chain | Residue |
| A | LEU43 |
| A | GLY44 |
| A | ILE45 |
| A | SER46 |
| A | GLY48 |
| A | GLN49 |
| A | ASP50 |
| A | SER51 |
| A | ARG78 |
| A | LEU79 |
| A | GLN84 |
| A | ARG139 |
| A | THR157 |
| A | LYS186 |
| A | PRO207 |
| A | THR208 |
| A | ALA209 |
| A | HOH2036 |
| A | HOH2039 |
| A | HOH2040 |
| A | HOH2057 |
| A | HOH2117 |
| A | HOH2118 |
| A | HOH2125 |
| A | HOH2128 |
| A | HOH2385 |
| A | HOH2386 |
| A | HOH2388 |
| A | MG4002 |
| A | MG4003 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1EE1, ECO:0007744|PDB:1IFX, ECO:0007744|PDB:1KQP, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | VAL33 | |
| B | VAL1033 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00193, ECO:0007744|PDB:1EE1, ECO:0007744|PDB:1FYD, ECO:0007744|PDB:1IH8, ECO:0007744|PDB:1KQP, ECO:0007744|PDB:1NSY, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | ILE45 | |
| A | ARG187 | |
| B | ILE1045 | |
| B | ARG1187 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00193, ECO:0007744|PDB:1KQP, ECO:0007744|PDB:1NSY, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | SER51 | |
| A | ALA163 | |
| B | SER1051 | |
| B | ALA1163 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1EE1, ECO:0007744|PDB:1FYD, ECO:0007744|PDB:1IH8, ECO:0007744|PDB:1KQP, ECO:0007744|PDB:1NSY, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | LEU79 | |
| A | GLN85 | |
| B | LEU1079 | |
| B | GLN1085 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | BINDING: in other chain => ECO:0000255|HAMAP-Rule:MF_00193, ECO:0007744|PDB:1EE1, ECO:0007744|PDB:1IFX, ECO:0007744|PDB:1KQP, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | THR138 | |
| A | TYR171 | |
| A | LYS258 | |
| B | THR1138 | |
| B | TYR1171 | |
| B | LYS1258 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00193, ECO:0007744|PDB:1EE1, ECO:0007744|PDB:1KQP, ECO:0007744|PDB:1NSY, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | ASP158 | |
| B | ASP1158 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00193, ECO:0007744|PDB:1EE1, ECO:0007744|PDB:1IFX, ECO:0007744|PDB:1KQP, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | LEU178 | |
| B | LEU1178 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00193, ECO:0007744|PDB:1EE1, ECO:0007744|PDB:1FYD, ECO:0007744|PDB:1IH8, ECO:0007744|PDB:1NSY, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | ALA209 | |
| B | ALA1209 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | BINDING: in other chain => ECO:0007744|PDB:1EE1, ECO:0007744|PDB:1KQP, ECO:0007744|PDB:2NSY |
| Chain | Residue | Details |
| A | LEU224 | |
| B | LEU1224 |
