Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0050661 | molecular_function | NADP binding |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0046452 | biological_process | dihydrofolate metabolic process |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0046655 | biological_process | folic acid metabolic process |
| B | 0050661 | molecular_function | NADP binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE NDP A 193 |
| Chain | Residue |
| A | ARG30 |
| A | SER94 |
| A | GLY114 |
| A | ALA115 |
| A | GLU116 |
| A | ILE117 |
| A | GLU120 |
| A | TQ5194 |
| A | HOH247 |
| A | HOH352 |
| A | HOH395 |
| A | LYS31 |
| A | HOH423 |
| A | HOH461 |
| A | HOH492 |
| A | GLY55 |
| A | ARG56 |
| A | LYS57 |
| A | THR58 |
| A | LEU77 |
| A | SER78 |
| A | ARG79 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE TQ5 A 194 |
| Chain | Residue |
| A | ILE9 |
| A | VAL10 |
| A | ALA11 |
| A | ILE19 |
| A | GLU32 |
| A | ILE33 |
| A | PHE36 |
| A | ILE112 |
| A | TYR118 |
| A | THR133 |
| A | NDP193 |
| site_id | AC3 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE NDP B 195 |
| Chain | Residue |
| B | GLY55 |
| B | ARG56 |
| B | LYS57 |
| B | THR58 |
| B | LEU77 |
| B | SER78 |
| B | ARG79 |
| B | SER94 |
| B | SER95 |
| B | ILE96 |
| B | GLY114 |
| B | ALA115 |
| B | GLU116 |
| B | ILE117 |
| B | GLU120 |
| B | TQ5196 |
| B | HOH264 |
| B | HOH350 |
| B | HOH389 |
| B | HOH440 |
| B | HOH481 |
| B | HOH490 |
| B | HOH537 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE TQ5 B 196 |
| Chain | Residue |
| B | ILE9 |
| B | VAL10 |
| B | ILE19 |
| B | MET25 |
| B | GLU32 |
| B | PHE36 |
| B | THR58 |
| B | ILE112 |
| B | TYR118 |
| B | THR133 |
| B | NDP195 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MES A 201 |
| Chain | Residue |
| A | PHE36 |
| A | LEU69 |
| A | ARG72 |
| A | HOH501 |
| A | HOH538 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MES B 202 |
| Chain | Residue |
| B | PHE36 |
| B | LYS37 |
| B | PRO70 |
| B | ARG72 |
| B | HOH301 |
| B | HOH541 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGykgkMPWrlrk.EiryFkdvT |
| Chain | Residue | Details |
| A | GLY18-THR40 | |
Functional Information from SwissProt/UniProt
| Chain | Residue | Details |
| A | ALA11 | |
| B | GLY113 | |
| A | GLY18 | |
| A | ARG56 | |
| A | SER78 | |
| A | GLY113 | |
| B | ALA11 | |
| B | GLY18 | |
| B | ARG56 | |
| B | SER78 | |
| Chain | Residue | Details |
| A | GLU32 | |
| A | ILE112 | |
| A | TYR118 | |
| B | GLU32 | |
| B | ILE112 | |
| B | TYR118 | |
| Chain | Residue | Details |
| A | ARG72 | |
| B | ARG72 | |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 7 |
| Details | Annotated By Reference To The Literature 1ra2 |
| Chain | Residue | Details |
| A | VAL10 | |
| A | GLU32 | |
| A | ILE33 | |
| A | MET25 | |
| A | ILE112 | |
| A | LEU69 | |
| A | PHE36 | |
| site_id | CSA2 |
| Number of Residues | 7 |
| Details | Annotated By Reference To The Literature 1ra2 |
| Chain | Residue | Details |
| B | VAL10 | |
| B | GLU32 | |
| B | ILE33 | |
| B | MET25 | |
| B | ILE112 | |
| B | LEU69 | |
| B | PHE36 | |
