1HYG
Crystal structure of MJ0490 gene product, the family of lactate/malate dehydrogenase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006089 | biological_process | lactate metabolic process |
| A | 0006099 | biological_process | tricarboxylic acid cycle |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| A | 0030060 | molecular_function | L-malate dehydrogenase (NAD+) activity |
| A | 0042867 | biological_process | pyruvate catabolic process |
| A | 0046554 | molecular_function | L-malate dehydrogenase (NADP+) activity |
| A | 0102443 | molecular_function | L-2-hydroxycarboxylate dehydrogenase (NAD+) activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006089 | biological_process | lactate metabolic process |
| B | 0006099 | biological_process | tricarboxylic acid cycle |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0030060 | molecular_function | L-malate dehydrogenase (NAD+) activity |
| B | 0042867 | biological_process | pyruvate catabolic process |
| B | 0046554 | molecular_function | L-malate dehydrogenase (NADP+) activity |
| B | 0102443 | molecular_function | L-2-hydroxycarboxylate dehydrogenase (NAD+) activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE NAP A 900 |
| Chain | Residue |
| A | GLY7 |
| A | THR81 |
| A | GLY83 |
| A | PRO85 |
| A | ILE102 |
| A | THR122 |
| A | ASN123 |
| A | HIS178 |
| A | GLY227 |
| A | SER228 |
| A | SER9 |
| A | GLY10 |
| A | ARG11 |
| A | VAL12 |
| A | GLY33 |
| A | ARG34 |
| A | HIS36 |
| A | SER37 |
| site_id | AC2 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE NAP B 901 |
| Chain | Residue |
| A | ASP115 |
| B | GLY7 |
| B | SER9 |
| B | GLY10 |
| B | ARG11 |
| B | VAL12 |
| B | ARG34 |
| B | HIS36 |
| B | SER37 |
| B | THR81 |
| B | SER82 |
| B | GLY83 |
| B | ILE102 |
| B | LYS105 |
| B | ILE121 |
| B | THR122 |
| B | ASN123 |
| B | LEU146 |
| B | ARG154 |
| B | HIS178 |
| B | GLY227 |
| B | SER228 |
| B | HOH736 |
| B | HOH758 |
Functional Information from PROSITE/UniProt
| site_id | PS00064 |
| Number of Residues | 7 |
| Details | L_LDH L-lactate dehydrogenase active site. IGEHGDS |
| Chain | Residue | Details |
| A | ILE175-SER181 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"UniProtKB","id":"P61889","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 10 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"11292347","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1HYG","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"1HYG","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P61889","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Site: {"description":"Discrimination site allowing the use of NADPH","evidences":[{"source":"PubMed","id":"10998181","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Site: {"description":"Discrimination site conferring specificity for malate over lactate","evidences":[{"source":"PubMed","id":"10998181","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| A | ASP151 | |
| A | HIS178 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| B | ASP151 | |
| B | HIS178 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| A | ASP151 | |
| A | ARG154 | |
| A | HIS178 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| B | ASP151 | |
| B | ARG154 | |
| B | HIS178 |
