1HY3
CRYSTAL STRUCTURE OF HUMAN ESTROGEN SULFOTRANSFERASE V269E MUTANT IN THE PRESENCE OF PAPS
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004062 | molecular_function | aryl sulfotransferase activity |
| A | 0004304 | molecular_function | estrone sulfotransferase activity |
| A | 0005496 | molecular_function | steroid binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006068 | biological_process | ethanol catabolic process |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006711 | biological_process | estrogen catabolic process |
| A | 0008146 | molecular_function | sulfotransferase activity |
| A | 0008202 | biological_process | steroid metabolic process |
| A | 0008210 | biological_process | estrogen metabolic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0031965 | cellular_component | nuclear membrane |
| A | 0045600 | biological_process | positive regulation of fat cell differentiation |
| A | 0047894 | molecular_function | flavonol 3-sulfotransferase activity |
| A | 0050294 | molecular_function | steroid sulfotransferase activity |
| A | 0050427 | biological_process | 3'-phosphoadenosine 5'-phosphosulfate metabolic process |
| A | 0051923 | biological_process | sulfation |
| B | 0004062 | molecular_function | aryl sulfotransferase activity |
| B | 0004304 | molecular_function | estrone sulfotransferase activity |
| B | 0005496 | molecular_function | steroid binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006068 | biological_process | ethanol catabolic process |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006711 | biological_process | estrogen catabolic process |
| B | 0008146 | molecular_function | sulfotransferase activity |
| B | 0008202 | biological_process | steroid metabolic process |
| B | 0008210 | biological_process | estrogen metabolic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0031965 | cellular_component | nuclear membrane |
| B | 0045600 | biological_process | positive regulation of fat cell differentiation |
| B | 0047894 | molecular_function | flavonol 3-sulfotransferase activity |
| B | 0050294 | molecular_function | steroid sulfotransferase activity |
| B | 0050427 | biological_process | 3'-phosphoadenosine 5'-phosphosulfate metabolic process |
| B | 0051923 | biological_process | sulfation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 27 |
| Details | BINDING SITE FOR RESIDUE PPS B 502 |
| Chain | Residue |
| B | PRO46 |
| B | ARG129 |
| B | SER137 |
| B | TYR192 |
| B | THR226 |
| B | PHE228 |
| B | MET231 |
| B | PHE254 |
| B | MET255 |
| B | ARG256 |
| B | LYS257 |
| B | LYS47 |
| B | GLY258 |
| B | HOH508 |
| B | HOH512 |
| B | HOH517 |
| B | HOH536 |
| B | HOH580 |
| B | HOH604 |
| B | HOH674 |
| B | SER48 |
| B | GLY49 |
| B | THR50 |
| B | THR51 |
| B | TRP52 |
| B | LYS105 |
| B | HIS107 |
| site_id | AC2 |
| Number of Residues | 27 |
| Details | BINDING SITE FOR RESIDUE PPS A 602 |
| Chain | Residue |
| A | LYS47 |
| A | SER48 |
| A | GLY49 |
| A | THR50 |
| A | THR51 |
| A | TRP52 |
| A | LYS105 |
| A | HIS107 |
| A | ARG129 |
| A | SER137 |
| A | PHE141 |
| A | TYR192 |
| A | THR226 |
| A | PHE228 |
| A | MET231 |
| A | PHE254 |
| A | MET255 |
| A | ARG256 |
| A | LYS257 |
| A | GLY258 |
| A | HOH608 |
| A | HOH611 |
| A | HOH617 |
| A | HOH620 |
| A | HOH634 |
| A | HOH644 |
| A | HOH740 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000269|PubMed:11006110 |
| Chain | Residue | Details |
| A | HIS107 | |
| B | HIS107 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P49891 |
| Chain | Residue | Details |
| A | ARG129 | |
| A | TYR192 | |
| A | THR226 | |
| A | ARG256 | |
| B | LYS47 | |
| B | LYS105 | |
| B | ARG129 | |
| B | TYR192 | |
| B | THR226 | |
| B | ARG256 | |
| A | LYS47 | |
| A | LYS105 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11884392 |
| Chain | Residue | Details |
| A | SER137 | |
| B | SER137 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 154 |
| Chain | Residue | Details |
| A | LYS47 | electrostatic stabiliser, hydrogen bond donor |
| A | HIS107 | hydrogen bond acceptor, proton acceptor, proton donor, proton relay |
| A | SER137 | hydrogen bond acceptor, steric role |
| site_id | MCSA2 |
| Number of Residues | 3 |
| Details | M-CSA 154 |
| Chain | Residue | Details |
| B | LYS47 | electrostatic stabiliser, hydrogen bond donor |
| B | HIS107 | hydrogen bond acceptor, proton acceptor, proton donor, proton relay |
| B | SER137 | hydrogen bond acceptor, steric role |
