1HM3

ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0016829	molecular_function	lyase activity
A	0030246	molecular_function	carbohydrate binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	Active site: {}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	4
Details	Binding site: {}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	2
Details	Glycosylation: {"description":"O-linked (Man...) serine"}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1c82

Chain	Residue	Details
A	ASN175
A	TYR234
A	HIS225

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site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1c82

Chain	Residue	Details
A	TYR234
A	HIS225
A	ARG288

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site_id	MCSA1
Number of Residues	5
Details	M-CSA 441

Chain	Residue	Details
A	ASN175	electrostatic stabiliser
A	HIS225	electrostatic stabiliser, modifies pKa
A	TYR234	proton shuttle (general acid/base)
A	ARG288	electrostatic stabiliser, modifies pKa
A	GLU371	modifies pKa

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