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1HDE

HALOALKANE DEHALOGENASE MUTANT WITH PHE 172 REPLACED WITH TRP

Functional Information from GO Data
ChainGOidnamespacecontents
A0003824molecular_functioncatalytic activity
A0009636biological_processresponse to toxic substance
A0016787molecular_functionhydrolase activity
A0018786molecular_functionhaloalkane dehalogenase activity
A0019260biological_process1,2-dichloroethane catabolic process
B0003824molecular_functioncatalytic activity
B0009636biological_processresponse to toxic substance
B0016787molecular_functionhydrolase activity
B0018786molecular_functionhaloalkane dehalogenase activity
B0019260biological_process1,2-dichloroethane catabolic process
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2
DetailsACT_SITE: Nucleophile
ChainResidueDetails
AASP124
BASP124

site_idSWS_FT_FI2
Number of Residues2
DetailsACT_SITE: Proton donor
ChainResidueDetails
AASP260
BASP260

site_idSWS_FT_FI3
Number of Residues2
DetailsACT_SITE: Proton acceptor
ChainResidueDetails
AHIS289
BHIS289

site_idSWS_FT_FI4
Number of Residues2
DetailsBINDING: BINDING => ECO:0000269|PubMed:10393294, ECO:0000269|PubMed:8515812
ChainResidueDetails
ATRP125
BTRP125

site_idSWS_FT_FI5
Number of Residues2
DetailsBINDING: BINDING => ECO:0000269|PubMed:10393294, ECO:0000269|PubMed:8515812, ECO:0007744|PDB:2DHD, ECO:0007744|PDB:2DHE
ChainResidueDetails
ATRP175
BTRP175

Catalytic Information from CSA
site_idCSA1
Number of Residues3
DetailsAnnotated By Reference To The Literature 1b6g
ChainResidueDetails
AHIS289
AASP124
AASP260

site_idCSA2
Number of Residues3
DetailsAnnotated By Reference To The Literature 1b6g
ChainResidueDetails
BHIS289
BASP124
BASP260

site_idMCSA1
Number of Residues5
DetailsM-CSA 415
ChainResidueDetails
AASP124covalent catalysis
ATRP125electrostatic stabiliser
ATRP175electrostatic stabiliser
AASP260activator, electrostatic stabiliser
AHIS289proton shuttle (general acid/base)

site_idMCSA2
Number of Residues5
DetailsM-CSA 415
ChainResidueDetails
BASP124covalent catalysis
BTRP125electrostatic stabiliser
BTRP175electrostatic stabiliser
BASP260activator, electrostatic stabiliser
BHIS289proton shuttle (general acid/base)

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PDB entries from 2024-10-30

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