1GYH
Structure of D158A Cellvibrio cellulosa alpha-L-arabinanase mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0046558 | molecular_function | arabinan endo-1,5-alpha-L-arabinosidase activity |
| B | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0046558 | molecular_function | arabinan endo-1,5-alpha-L-arabinosidase activity |
| C | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0046558 | molecular_function | arabinan endo-1,5-alpha-L-arabinosidase activity |
| D | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0046558 | molecular_function | arabinan endo-1,5-alpha-L-arabinosidase activity |
| E | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| E | 0005975 | biological_process | carbohydrate metabolic process |
| E | 0046558 | molecular_function | arabinan endo-1,5-alpha-L-arabinosidase activity |
| F | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| F | 0005975 | biological_process | carbohydrate metabolic process |
| F | 0046558 | molecular_function | arabinan endo-1,5-alpha-L-arabinosidase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CL A1348 |
| Chain | Residue |
| A | HIS291 |
| A | HOH2132 |
| A | HOH2230 |
| A | HOH2231 |
| A | HOH2307 |
| A | HOH2376 |
| A | HOH2377 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CL B1348 |
| Chain | Residue |
| B | HOH2095 |
| B | HOH2173 |
| B | HOH2234 |
| B | HOH2293 |
| B | HOH2294 |
| B | HIS291 |
| B | HOH2093 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CL C1348 |
| Chain | Residue |
| C | HIS291 |
| C | HOH2092 |
| C | HOH2178 |
| C | HOH2179 |
| C | HOH2249 |
| C | HOH2309 |
| C | HOH2310 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CL D1347 |
| Chain | Residue |
| D | HIS291 |
| D | HOH2065 |
| D | HOH2066 |
| D | HOH2127 |
| D | HOH2203 |
| D | HOH2246 |
| D | HOH2247 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CL E1348 |
| Chain | Residue |
| E | HIS291 |
| E | HOH2077 |
| E | HOH2138 |
| E | HOH2139 |
| E | HOH2197 |
| E | HOH2241 |
| E | HOH2242 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CL F1347 |
| Chain | Residue |
| F | HIS291 |
| F | HOH2034 |
| F | HOH2035 |
| F | HOH2074 |
| F | HOH2075 |
| F | HOH2150 |
| F | HOH2151 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"12198486","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"12198486","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 48 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"12198486","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | Site: {"description":"Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate","evidences":[{"source":"PubMed","id":"12198486","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | Site: {"description":"Important for substrate recognition and stabilization"} |
| Chain | Residue | Details |
