1GS5
N-acetyl-L-glutamate kinase from Escherichia coli complexed with its substrate N-acetylglutamate and its substrate analog AMPPNP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003991 | molecular_function | acetylglutamate kinase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006526 | biological_process | L-arginine biosynthetic process |
| A | 0006974 | biological_process | DNA damage response |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0042450 | biological_process | L-arginine biosynthetic process via ornithine |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG A 1261 |
| Chain | Residue |
| A | ANP1260 |
| A | HOH2135 |
| A | HOH2195 |
| site_id | AC2 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE NLG A 1259 |
| Chain | Residue |
| A | ASN158 |
| A | VAL159 |
| A | ASN160 |
| A | ALA161 |
| A | ANP1260 |
| A | HOH2194 |
| A | HOH2195 |
| A | HOH2196 |
| A | GLY43 |
| A | GLY44 |
| A | GLY45 |
| A | LEU65 |
| A | ARG66 |
| A | LEU80 |
| site_id | AC3 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE ANP A 1260 |
| Chain | Residue |
| A | LYS8 |
| A | GLY10 |
| A | GLY11 |
| A | GLY43 |
| A | GLY44 |
| A | GLY45 |
| A | SER180 |
| A | VAL182 |
| A | LEU186 |
| A | ILE209 |
| A | ILE210 |
| A | THR211 |
| A | MET214 |
| A | LYS217 |
| A | NLG1259 |
| A | MG1261 |
| A | HOH2197 |
| A | HOH2198 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00082","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12005432","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12875848","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00082","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12875848","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"source":"HAMAP-Rule","id":"MF_00082","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12875848","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1oh9 |
| Chain | Residue | Details |
| A | LYS8 | |
| A | GLY45 | |
| A | GLY11 | |
| A | LYS217 |
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 870 |
| Chain | Residue | Details |
| A | LYS8 | transition state stabiliser |
| A | GLY11 | transition state stabiliser |
| A | GLY45 | transition state stabiliser |
| A | ASP162 | activator, steric role |
| A | LYS217 | transition state stabiliser |
