Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1GK2

Histidine Ammonia-Lyase (HAL) Mutant F329G from Pseudomonas putida

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004397	molecular_function	histidine ammonia-lyase activity
A	0005737	cellular_component	cytoplasm
A	0006548	biological_process	L-histidine catabolic process
A	0016841	molecular_function	ammonia-lyase activity
B	0003824	molecular_function	catalytic activity
B	0004397	molecular_function	histidine ammonia-lyase activity
B	0005737	cellular_component	cytoplasm
B	0006548	biological_process	L-histidine catabolic process
B	0016841	molecular_function	ammonia-lyase activity
C	0003824	molecular_function	catalytic activity
C	0004397	molecular_function	histidine ammonia-lyase activity
C	0005737	cellular_component	cytoplasm
C	0006548	biological_process	L-histidine catabolic process
C	0016841	molecular_function	ammonia-lyase activity
D	0003824	molecular_function	catalytic activity
D	0004397	molecular_function	histidine ammonia-lyase activity
D	0005737	cellular_component	cytoplasm
D	0006548	biological_process	L-histidine catabolic process
D	0016841	molecular_function	ammonia-lyase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 1511

Chain	Residue
A	TYR53
A	SER143
A	ASN313
A	SER325
D	TYR280
D	ARG283

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 1511

Chain	Residue
C	TYR280
C	ARG283
B	TYR53
B	ASN313
B	SER325

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 1512

Chain	Residue
B	TYR280
B	ARG283
C	SER143
C	ASN195
C	ASN313
C	HOH2103

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 1511

Chain	Residue
A	TYR280
A	ARG283
D	TYR53
D	ASN195
D	ASN313

site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 1510

Chain	Residue
A	GLY54
A	ILE55
A	THR57
A	GLN76
A	LEU79
A	LYS188
A	GLU189
A	GLY190
A	LEU191

site_id	AC6
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL B 1510

Chain	Residue
B	GLY54
B	ILE55
B	THR57
B	PHE59
B	LEU72
B	GLN76
B	LEU79
B	LYS188
B	GLY190
B	LEU191
B	HOH2298

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL C 1510

Chain	Residue
C	ILE55
C	PHE59
C	GLN76
C	LEU79
C	ALA187
C	LYS188
C	GLY190
C	LEU191
C	HOH2050

site_id	AC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL D 1510

Chain	Residue
D	GLY54
D	ILE55
D	LEU72
D	GLN76
D	ALA187
D	LYS188
D	GLU189
D	GLY190
D	LEU191
D	HOH2290

Functional Information from PROSITE/UniProt

site_id	PS00213
Number of Residues	14
Details	LIPOCALIN Lipocalin signature. GEGkaRYKGQWLSA

Chain	Residue	Details
A	GLY158-ALA171

site_id	PS00488
Number of Residues	17
Details	PAL_HISTIDASE Phenylalanine and histidine ammonia-lyases signature. GSVGASGDLaPLAhmsL

Chain	Residue	Details
A	GLY138-LEU154

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Modified residue: {"description":"2,3-didehydroalanine (Ser)","evidences":[{"source":"PubMed","id":"10220322","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7947813","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8204579","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Cross-link: {"description":"5-imidazolinone (Ala-Gly)","evidences":[{"source":"PubMed","id":"10220322","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1b8f

Chain	Residue	Details
A	ALA142
A	TYR280
A	SER143
A	GLU414
A	GLY144

site_id	CSA2
Number of Residues	5
Details	Annotated By Reference To The Literature 1b8f

Chain	Residue	Details
B	ALA142
B	TYR280
B	SER143
B	GLU414
B	GLY144

site_id	CSA3
Number of Residues	5
Details	Annotated By Reference To The Literature 1b8f

Chain	Residue	Details
C	ALA142
C	TYR280
C	SER143
C	GLU414
C	GLY144

site_id	CSA4
Number of Residues	5
Details	Annotated By Reference To The Literature 1b8f

Chain	Residue	Details
D	ALA142
D	TYR280
D	SER143
D	GLU414
D	GLY144

site_id	MCSA1
Number of Residues	7
Details	M-CSA 417

Chain	Residue	Details
A	TYR53	electrostatic stabiliser
A	HIS83	electrostatic stabiliser
A	ASN195	activator
A	TYR280	proton shuttle (general acid/base)
A	ARG283	electrostatic stabiliser
A	GLY329	electrostatic stabiliser
A	GLU414	proton shuttle (general acid/base)

site_id	MCSA2
Number of Residues	7
Details	M-CSA 417

Chain	Residue	Details
B	TYR53	electrostatic stabiliser
B	HIS83	electrostatic stabiliser
B	ASN195	activator
B	TYR280	proton shuttle (general acid/base)
B	ARG283	electrostatic stabiliser
B	GLY329	electrostatic stabiliser
B	GLU414	proton shuttle (general acid/base)

site_id	MCSA3
Number of Residues	7
Details	M-CSA 417

Chain	Residue	Details
C	TYR53	electrostatic stabiliser
C	HIS83	electrostatic stabiliser
C	ASN195	activator
C	TYR280	proton shuttle (general acid/base)
C	ARG283	electrostatic stabiliser
C	GLY329	electrostatic stabiliser
C	GLU414	proton shuttle (general acid/base)

site_id	MCSA4
Number of Residues	7
Details	M-CSA 417

Chain	Residue	Details
D	TYR53	electrostatic stabiliser
D	HIS83	electrostatic stabiliser
D	ASN195	activator
D	TYR280	proton shuttle (general acid/base)
D	ARG283	electrostatic stabiliser
D	GLY329	electrostatic stabiliser
D	GLU414	proton shuttle (general acid/base)

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)