1GK2
Histidine Ammonia-Lyase (HAL) Mutant F329G from Pseudomonas putida
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004397 | molecular_function | histidine ammonia-lyase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006547 | biological_process | L-histidine metabolic process |
| A | 0006548 | biological_process | L-histidine catabolic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016841 | molecular_function | ammonia-lyase activity |
| A | 0019556 | biological_process | L-histidine catabolic process to glutamate and formamide |
| A | 0019557 | biological_process | L-histidine catabolic process to glutamate and formate |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004397 | molecular_function | histidine ammonia-lyase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006547 | biological_process | L-histidine metabolic process |
| B | 0006548 | biological_process | L-histidine catabolic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016841 | molecular_function | ammonia-lyase activity |
| B | 0019556 | biological_process | L-histidine catabolic process to glutamate and formamide |
| B | 0019557 | biological_process | L-histidine catabolic process to glutamate and formate |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004397 | molecular_function | histidine ammonia-lyase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006547 | biological_process | L-histidine metabolic process |
| C | 0006548 | biological_process | L-histidine catabolic process |
| C | 0016829 | molecular_function | lyase activity |
| C | 0016841 | molecular_function | ammonia-lyase activity |
| C | 0019556 | biological_process | L-histidine catabolic process to glutamate and formamide |
| C | 0019557 | biological_process | L-histidine catabolic process to glutamate and formate |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004397 | molecular_function | histidine ammonia-lyase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006547 | biological_process | L-histidine metabolic process |
| D | 0006548 | biological_process | L-histidine catabolic process |
| D | 0016829 | molecular_function | lyase activity |
| D | 0016841 | molecular_function | ammonia-lyase activity |
| D | 0019556 | biological_process | L-histidine catabolic process to glutamate and formamide |
| D | 0019557 | biological_process | L-histidine catabolic process to glutamate and formate |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1511 |
| Chain | Residue |
| A | TYR53 |
| A | SER143 |
| A | ASN313 |
| A | SER325 |
| D | TYR280 |
| D | ARG283 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1511 |
| Chain | Residue |
| C | TYR280 |
| C | ARG283 |
| B | TYR53 |
| B | ASN313 |
| B | SER325 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1512 |
| Chain | Residue |
| B | TYR280 |
| B | ARG283 |
| C | SER143 |
| C | ASN195 |
| C | ASN313 |
| C | HOH2103 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1511 |
| Chain | Residue |
| A | TYR280 |
| A | ARG283 |
| D | TYR53 |
| D | ASN195 |
| D | ASN313 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL A 1510 |
| Chain | Residue |
| A | GLY54 |
| A | ILE55 |
| A | THR57 |
| A | GLN76 |
| A | LEU79 |
| A | LYS188 |
| A | GLU189 |
| A | GLY190 |
| A | LEU191 |
| site_id | AC6 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE GOL B 1510 |
| Chain | Residue |
| B | GLY54 |
| B | ILE55 |
| B | THR57 |
| B | PHE59 |
| B | LEU72 |
| B | GLN76 |
| B | LEU79 |
| B | LYS188 |
| B | GLY190 |
| B | LEU191 |
| B | HOH2298 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL C 1510 |
| Chain | Residue |
| C | ILE55 |
| C | PHE59 |
| C | GLN76 |
| C | LEU79 |
| C | ALA187 |
| C | LYS188 |
| C | GLY190 |
| C | LEU191 |
| C | HOH2050 |
| site_id | AC8 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GOL D 1510 |
| Chain | Residue |
| D | GLY54 |
| D | ILE55 |
| D | LEU72 |
| D | GLN76 |
| D | ALA187 |
| D | LYS188 |
| D | GLU189 |
| D | GLY190 |
| D | LEU191 |
| D | HOH2290 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | MOD_RES: 2,3-didehydroalanine (Ser) => ECO:0000269|PubMed:7947813, ECO:0000269|PubMed:8204579 |
| Chain | Residue | Details |
| A | SER143 | |
| B | SER143 | |
| C | SER143 | |
| D | SER143 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | CROSSLNK: 5-imidazolinone (Ala-Gly) |
| Chain | Residue | Details |
| A | ALA142 | |
| A | GLY144 | |
| B | ALA142 | |
| B | GLY144 | |
| C | ALA142 | |
| C | GLY144 | |
| D | ALA142 | |
| D | GLY144 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1b8f |
| Chain | Residue | Details |
| A | ALA142 | |
| A | TYR280 | |
| A | SER143 | |
| A | GLU414 | |
| A | GLY144 |
| site_id | CSA2 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1b8f |
| Chain | Residue | Details |
| B | ALA142 | |
| B | TYR280 | |
| B | SER143 | |
| B | GLU414 | |
| B | GLY144 |
| site_id | CSA3 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1b8f |
| Chain | Residue | Details |
| C | ALA142 | |
| C | TYR280 | |
| C | SER143 | |
| C | GLU414 | |
| C | GLY144 |
| site_id | CSA4 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1b8f |
| Chain | Residue | Details |
| D | ALA142 | |
| D | TYR280 | |
| D | SER143 | |
| D | GLU414 | |
| D | GLY144 |
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 417 |
| Chain | Residue | Details |
| A | TYR53 | electrostatic stabiliser |
| A | HIS83 | electrostatic stabiliser |
| A | ASN195 | activator |
| A | TYR280 | proton shuttle (general acid/base) |
| A | ARG283 | electrostatic stabiliser |
| A | GLY329 | electrostatic stabiliser |
| A | GLU414 | proton shuttle (general acid/base) |
| site_id | MCSA2 |
| Number of Residues | 7 |
| Details | M-CSA 417 |
| Chain | Residue | Details |
| B | TYR53 | electrostatic stabiliser |
| B | HIS83 | electrostatic stabiliser |
| B | ASN195 | activator |
| B | TYR280 | proton shuttle (general acid/base) |
| B | ARG283 | electrostatic stabiliser |
| B | GLY329 | electrostatic stabiliser |
| B | GLU414 | proton shuttle (general acid/base) |
| site_id | MCSA3 |
| Number of Residues | 7 |
| Details | M-CSA 417 |
| Chain | Residue | Details |
| C | TYR53 | electrostatic stabiliser |
| C | HIS83 | electrostatic stabiliser |
| C | ASN195 | activator |
| C | TYR280 | proton shuttle (general acid/base) |
| C | ARG283 | electrostatic stabiliser |
| C | GLY329 | electrostatic stabiliser |
| C | GLU414 | proton shuttle (general acid/base) |
| site_id | MCSA4 |
| Number of Residues | 7 |
| Details | M-CSA 417 |
| Chain | Residue | Details |
| D | TYR53 | electrostatic stabiliser |
| D | HIS83 | electrostatic stabiliser |
| D | ASN195 | activator |
| D | TYR280 | proton shuttle (general acid/base) |
| D | ARG283 | electrostatic stabiliser |
| D | GLY329 | electrostatic stabiliser |
| D | GLU414 | proton shuttle (general acid/base) |
