1GK0
Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0016787 | molecular_function | hydrolase activity |
A | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
A | 0017000 | biological_process | antibiotic biosynthetic process |
B | 0016787 | molecular_function | hydrolase activity |
B | 0017000 | biological_process | antibiotic biosynthetic process |
C | 0016787 | molecular_function | hydrolase activity |
C | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
C | 0017000 | biological_process | antibiotic biosynthetic process |
D | 0016787 | molecular_function | hydrolase activity |
D | 0017000 | biological_process | antibiotic biosynthetic process |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE PO4 B1523 |
Chain | Residue |
B | SER1 |
B | HIS23 |
B | LEU24 |
B | VAL70 |
B | ASN244 |
B | EDO1524 |
B | HOH2261 |
B | HOH2262 |
site_id | AC2 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE EDO B1524 |
Chain | Residue |
B | SER1 |
B | TYR33 |
B | ARG57 |
B | PHE177 |
B | PO41523 |
A | TYR150 |
site_id | AC3 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE PO4 D1523 |
Chain | Residue |
D | SER1 |
D | HIS23 |
D | LEU24 |
D | VAL70 |
D | ASN244 |
D | EDO1524 |
D | HOH2269 |
D | HOH2271 |
site_id | AC4 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE EDO D1524 |
Chain | Residue |
C | TYR150 |
D | TYR33 |
D | ARG57 |
D | PHE58 |
D | PO41523 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 2 |
Details | ACT_SITE: Nucleophile |
Chain | Residue | Details |
B | SER1 | |
D | SER1 |
site_id | SWS_FT_FI2 |
Number of Residues | 4 |
Details | ACT_SITE: ACT_SITE => ECO:0000255 |
Chain | Residue | Details |
B | HIS23 | |
B | GLU455 | |
D | HIS23 | |
D | GLU455 |
Catalytic Information from CSA
site_id | CSA1 |
Number of Residues | 2 |
Details | Annotated By Reference To The Literature 3s8r |
Chain | Residue | Details |
B | SER1 | |
B | ASN244 |
site_id | CSA2 |
Number of Residues | 2 |
Details | Annotated By Reference To The Literature 3s8r |
Chain | Residue | Details |
D | SER1 | |
D | ASN244 |
site_id | MCSA1 |
Number of Residues | 5 |
Details | M-CSA 288 |
Chain | Residue | Details |
B | SER1 | activator, covalently attached, hydrogen bond acceptor, hydrogen bond donor, increase electrophilicity, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
B | HIS23 | activator, electrostatic stabiliser, increase acidity, steric role |
B | VAL70 | electrostatic stabiliser |
B | ASN244 | electrostatic stabiliser |
B | GLU455 | activator, electrostatic stabiliser, increase acidity, steric role |
site_id | MCSA2 |
Number of Residues | 5 |
Details | M-CSA 288 |
Chain | Residue | Details |
D | SER1 | activator, covalently attached, hydrogen bond acceptor, hydrogen bond donor, increase electrophilicity, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
D | HIS23 | activator, electrostatic stabiliser, increase acidity, steric role |
D | VAL70 | electrostatic stabiliser |
D | ASN244 | electrostatic stabiliser |
D | GLU455 | activator, electrostatic stabiliser, increase acidity, steric role |