1GGR
COMPLEX OF ENZYME IIAGLC AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0009401 | biological_process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
| A | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| A | 0016020 | cellular_component | membrane |
| A | 0016301 | molecular_function | kinase activity |
| A | 0034763 | biological_process | negative regulation of transmembrane transport |
| A | 0043610 | biological_process | regulation of carbohydrate utilization |
| A | 0045912 | biological_process | negative regulation of carbohydrate metabolic process |
| A | 1902344 | biological_process | negative regulation of maltose transport |
| B | 0004857 | molecular_function | enzyme inhibitor activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0008047 | molecular_function | enzyme activator activity |
| B | 0009401 | biological_process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
| B | 0016775 | molecular_function | phosphotransferase activity, nitrogenous group as acceptor |
| B | 0018106 | biological_process | peptidyl-histidine phosphorylation |
| B | 0030234 | molecular_function | enzyme regulator activity |
| B | 0043609 | biological_process | regulation of carbon utilization |
| B | 0045152 | molecular_function | antisigma factor binding |
| B | 0045819 | biological_process | positive regulation of glycogen catabolic process |
Functional Information from PROSITE/UniProt
| site_id | PS00369 |
| Number of Residues | 8 |
| Details | PTS_HPR_HIS PTS HPR domain histidine phosphorylation site signature. GLHTRPAA |
| Chain | Residue | Details |
| B | GLY313-ALA320 |
| site_id | PS00371 |
| Number of Residues | 13 |
| Details | PTS_EIIA_TYPE_1_HIS PTS EIIA domains phosphorylation site signature 1. GveLFVHFGIdTV |
| Chain | Residue | Details |
| A | GLY84-VAL96 |
| site_id | PS00589 |
| Number of Residues | 16 |
| Details | PTS_HPR_SER PTS HPR domain serine phosphorylation site signature. GKsASaKSLFKLQtLG |
| Chain | Residue | Details |
| B | GLY339-GLY354 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 104 |
| Details | Domain: {"description":"PTS EIIA type-1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00416","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Tele-phosphohistidine intermediate; for EIIA activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00416","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"2657735","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"6383826","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"1961703","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9405042","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"2657735","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8170944","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9405042","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"1961703","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Site: {"description":"Important for phospho-donor activity","evidences":[{"source":"PubMed","id":"2657735","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphohistidine; by HPr","evidences":[{"source":"PubMed","id":"2657735","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 84 |
| Details | Domain: {"description":"HPr","evidences":[{"source":"PROSITE-ProRule","id":"PRU00681","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Pros-phosphohistidine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00681","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"2261470","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1gpr |
| Chain | Residue | Details |
| A | HIS90 | |
| A | HIS75 | |
| A | GLY92 | |
| A | THR73 |
