Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1GC3

THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 2 COMPLEXED WITH TRYPTOPHAN

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
A	0005737	cellular_component	cytoplasm
A	0008483	molecular_function	transaminase activity
A	0009058	biological_process	biosynthetic process
A	0030170	molecular_function	pyridoxal phosphate binding
A	0033853	molecular_function	aspartate-prephenate aminotransferase activity
B	0003824	molecular_function	catalytic activity
B	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
B	0005737	cellular_component	cytoplasm
B	0008483	molecular_function	transaminase activity
B	0009058	biological_process	biosynthetic process
B	0030170	molecular_function	pyridoxal phosphate binding
B	0033853	molecular_function	aspartate-prephenate aminotransferase activity
C	0003824	molecular_function	catalytic activity
C	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
C	0005737	cellular_component	cytoplasm
C	0008483	molecular_function	transaminase activity
C	0009058	biological_process	biosynthetic process
C	0030170	molecular_function	pyridoxal phosphate binding
C	0033853	molecular_function	aspartate-prephenate aminotransferase activity
D	0003824	molecular_function	catalytic activity
D	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
D	0005737	cellular_component	cytoplasm
D	0008483	molecular_function	transaminase activity
D	0009058	biological_process	biosynthetic process
D	0030170	molecular_function	pyridoxal phosphate binding
D	0033853	molecular_function	aspartate-prephenate aminotransferase activity
E	0003824	molecular_function	catalytic activity
E	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
E	0005737	cellular_component	cytoplasm
E	0008483	molecular_function	transaminase activity
E	0009058	biological_process	biosynthetic process
E	0030170	molecular_function	pyridoxal phosphate binding
E	0033853	molecular_function	aspartate-prephenate aminotransferase activity
F	0003824	molecular_function	catalytic activity
F	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
F	0005737	cellular_component	cytoplasm
F	0008483	molecular_function	transaminase activity
F	0009058	biological_process	biosynthetic process
F	0030170	molecular_function	pyridoxal phosphate binding
F	0033853	molecular_function	aspartate-prephenate aminotransferase activity
G	0003824	molecular_function	catalytic activity
G	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
G	0005737	cellular_component	cytoplasm
G	0008483	molecular_function	transaminase activity
G	0009058	biological_process	biosynthetic process
G	0030170	molecular_function	pyridoxal phosphate binding
G	0033853	molecular_function	aspartate-prephenate aminotransferase activity
H	0003824	molecular_function	catalytic activity
H	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
H	0005737	cellular_component	cytoplasm
H	0008483	molecular_function	transaminase activity
H	0009058	biological_process	biosynthetic process
H	0030170	molecular_function	pyridoxal phosphate binding
H	0033853	molecular_function	aspartate-prephenate aminotransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE TRP A 414

Chain	Residue
A	VAL16
A	PLP413
A	ASN20
A	THR37
A	ALA38
A	GLY39
A	GLU40
A	ASN175
A	TYR322
A	ARG361

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE TRP A 914

Chain	Residue
A	TYR64
A	THR265
B	VAL516
B	GLY539
B	ASN675
B	LYS734
B	TYR822
B	ARG861
B	PLP913

site_id	AC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE TRP C 1414

Chain	Residue
C	VAL1016
C	ASN1020
C	GLY1039
C	LYS1234
C	TYR1322
C	ARG1361
C	PLP1413
D	TYR1564

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE TRP D 1914

Chain	Residue
C	TYR1064
D	VAL1516
D	ASN1520
D	THR1537
D	GLY1539
D	ASN1675
D	LYS1734
D	TYR1822
D	ARG1861
D	PLP1913

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE TRP E 2414

Chain	Residue
E	GLY2039
E	TYR2322
E	ARG2361
E	PLP2413

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE TRP E 2914

Chain	Residue
E	TYR2064
F	VAL2516
F	GLY2539
F	LYS2734
F	TYR2822
F	ARG2861
F	PLP2913

site_id	AC7
Number of Residues	10
Details	BINDING SITE FOR RESIDUE TRP G 3414

Chain	Residue
G	VAL3016
G	ASN3020
G	THR3037
G	GLY3039
G	GLU3040
G	TRP3125
G	ASN3175
G	ARG3361
G	PLP3413
H	TYR3564

site_id	AC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE TRP H 3914

Chain	Residue
H	VAL3516
H	ASN3520
H	THR3537
H	GLY3539
H	ASN3675
H	TYR3822
H	ARG3861
H	PLP3913

site_id	AC9
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PLP A 413

Chain	Residue
A	GLY99
A	GLY100
A	SER101
A	ASN171
A	ASN175
A	ASP203
A	TYR206
A	LYS234
A	ARG242
A	TYR322
A	TRP414
B	TYR564

site_id	BC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PLP B 913

Chain	Residue
A	TYR64
A	TRP914
B	GLY600
B	SER601
B	TRP625
B	ASN671
B	ASN675
B	ASP703
B	TYR706
B	LYS734
B	ARG742

site_id	BC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PLP C 1413

Chain	Residue
C	TRP1414
D	TYR1564
C	GLY1100
C	SER1101
C	TRP1125
C	ASN1175
C	ASP1203
C	TYR1206
C	ALA1233
C	LYS1234
C	ARG1242
C	TYR1322

site_id	BC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PLP D 1913

Chain	Residue
C	TYR1064
D	GLY1600
D	SER1601
D	ASN1671
D	ASN1675
D	ASP1703
D	TYR1706
D	LYS1734
D	ARG1742
D	TRP1914

site_id	BC4
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PLP E 2413

Chain	Residue
E	GLY2099
E	GLY2100
E	SER2101
E	TRP2125
E	ASN2175
E	ASP2203
E	TYR2206
E	ALA2233
E	ARG2242
E	TYR2322
E	TRP2414
F	TYR2564

site_id	BC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PLP F 2913

Chain	Residue
E	TYR2064
E	TRP2914
F	GLY2600
F	SER2601
F	TRP2625
F	ASN2675
F	ASP2703
F	TYR2706
F	LYS2734
F	ARG2742

site_id	BC6
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PLP G 3413

Chain	Residue
G	GLY3100
G	SER3101
G	TRP3125
G	ASN3171
G	ASN3175
G	ASP3203
G	TYR3206
G	ARG3242
G	TRP3414
H	TYR3564

site_id	BC7
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PLP H 3913

Chain	Residue
G	TYR3064
H	GLY3599
H	GLY3600
H	SER3601
H	TRP3625
H	ASN3675
H	ASP3703
H	TYR3706
H	LYS3734
H	ARG3742
H	TYR3822
H	TRP3914

Functional Information from PROSITE/UniProt

site_id	PS00105
Number of Residues	14
Details	AA_TRANSFER_CLASS_1 Aminotransferases class-I pyridoxal-phosphate attachment site. GAAKafAMtGWRIG

Chain	Residue	Details
A	GLY231-GLY244

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P00509

Chain	Residue	Details
A	GLY39
B	GLY539
C	GLY1039
D	GLY1539
E	GLY2039
F	GLY2539
G	GLY3039
H	GLY3539

site_id	SWS_FT_FI2
Number of Residues	24
Details	BINDING: BINDING => ECO:0007744\|PDB:1GCK

Chain	Residue	Details
A	TRP125
D	TRP1625
D	ASN1675
D	ARG1861
E	TRP2125
E	ASN2175
E	ARG2361
F	TRP2625
F	ASN2675
F	ARG2861
G	TRP3125
A	ASN175
G	ASN3175
G	ARG3361
H	TRP3625
H	ASN3675
H	ARG3861
A	ARG361
B	TRP625
B	ASN675
B	ARG861
C	TRP1125
C	ASN1175
C	ARG1361

site_id	SWS_FT_FI3
Number of Residues	8
Details	SITE: Important for prephenate aminotransferase activity => ECO:0000269\|PubMed:30771275

Chain	Residue	Details
A	LYS12
B	LYS512
C	LYS1012
D	LYS1512
E	LYS2012
F	LYS2512
G	LYS3012
H	LYS3512

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269\|PubMed:10029535, ECO:0000269\|PubMed:11432784, ECO:0007744\|PDB:1B5O, ECO:0007744\|PDB:1B5P, ECO:0007744\|PDB:5BJ3, ECO:0007744\|PDB:5BJ4

Chain	Residue	Details
A	LYS234
B	LYS734
C	LYS1234
D	LYS1734
E	LYS2234
F	LYS2734
G	LYS3234
H	LYS3734

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
A	TYR124
A	ASP203

site_id	CSA10
Number of Residues	1
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
B	ALA568

site_id	CSA11
Number of Residues	1
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
C	ALA1068

site_id	CSA12
Number of Residues	1
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
D	ALA1568

site_id	CSA13
Number of Residues	1
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
E	ALA2068

site_id	CSA14
Number of Residues	1
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
F	ALA2568

site_id	CSA15
Number of Residues	1
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
G	ALA3068

site_id	CSA16
Number of Residues	1
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
H	ALA3568

site_id	CSA17
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
A	LYS234
A	TRP125
A	ASP203

site_id	CSA18
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
B	LYS734
B	TRP625
B	ASP703

site_id	CSA19
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
C	ASP1203
C	LYS1234
C	TRP1125

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
B	TYR624
B	ASP703

site_id	CSA20
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
D	ASP1703
D	LYS1734
D	TRP1625

site_id	CSA21
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
E	TRP2125
E	ASP2203
E	LYS2234

site_id	CSA22
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
F	TRP2625
F	ASP2703
F	LYS2734

site_id	CSA23
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
G	TRP3125
G	ASP3203
G	LYS3234

site_id	CSA24
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
H	TRP3625
H	ASP3703
H	LYS3734

site_id	CSA25
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
A	LYS234
A	TYR124
A	ASP203

site_id	CSA26
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
B	LYS734
B	TYR624
B	ASP703

site_id	CSA27
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
C	TYR1124
C	ASP1203
C	LYS1234

site_id	CSA28
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
D	ASP1703
D	TYR1624
D	LYS1734

site_id	CSA29
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
E	TYR2124
E	ASP2203
E	LYS2234

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
C	TYR1124
C	ASP1203

site_id	CSA30
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
F	ASP2703
F	TYR2624
F	LYS2734

site_id	CSA31
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
G	TYR3124
G	ASP3203
G	LYS3234

site_id	CSA32
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
H	ASP3703
H	LYS3734
H	TYR3624

site_id	CSA33
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
A	TRP125
A	ASP203

site_id	CSA34
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
B	TRP625
B	ASP703

site_id	CSA35
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
C	ASP1203
C	TRP1125

site_id	CSA36
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
D	ASP1703
D	TRP1625

site_id	CSA37
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
E	TRP2125
E	ASP2203

site_id	CSA38
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
F	TRP2625
F	ASP2703

site_id	CSA39
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
G	TRP3125
G	ASP3203

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
D	ASP1703
D	TYR1624

site_id	CSA40
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
H	TRP3625
H	ASP3703

site_id	CSA41
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
A	LYS234
A	ASP203
A	TYR128

site_id	CSA42
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
B	LYS734
B	TYR628
B	ASP703

site_id	CSA43
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
C	ASP1203
C	LYS1234
C	TYR1128

site_id	CSA44
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
D	TYR1628
D	ASP1703
D	LYS1734

site_id	CSA45
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
E	TYR2128
E	ASP2203
E	LYS2234

site_id	CSA46
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
F	ASP2703
F	TYR2628
F	LYS2734

site_id	CSA47
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
G	TYR3128
G	ASP3203
G	LYS3234

site_id	CSA48
Number of Residues	3
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
H	TYR3628
H	ASP3703
H	LYS3734

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
E	TYR2124
E	ASP2203

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
F	ASP2703
F	TYR2624

site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
G	TYR3124
G	ASP3203

site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
H	ASP3703
H	TYR3624

site_id	CSA9
Number of Residues	1
Details	Annotated By Reference To The Literature 1ay4

Chain	Residue	Details
A	ALA68

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)