1GC3
THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 2 COMPLEXED WITH TRYPTOPHAN
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006520 | biological_process | amino acid metabolic process |
| A | 0008483 | molecular_function | transaminase activity |
| A | 0009058 | biological_process | biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0030170 | molecular_function | pyridoxal phosphate binding |
| A | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006520 | biological_process | amino acid metabolic process |
| B | 0008483 | molecular_function | transaminase activity |
| B | 0009058 | biological_process | biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0030170 | molecular_function | pyridoxal phosphate binding |
| B | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006520 | biological_process | amino acid metabolic process |
| C | 0008483 | molecular_function | transaminase activity |
| C | 0009058 | biological_process | biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0030170 | molecular_function | pyridoxal phosphate binding |
| C | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006520 | biological_process | amino acid metabolic process |
| D | 0008483 | molecular_function | transaminase activity |
| D | 0009058 | biological_process | biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0030170 | molecular_function | pyridoxal phosphate binding |
| D | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0006520 | biological_process | amino acid metabolic process |
| E | 0008483 | molecular_function | transaminase activity |
| E | 0009058 | biological_process | biosynthetic process |
| E | 0016740 | molecular_function | transferase activity |
| E | 0030170 | molecular_function | pyridoxal phosphate binding |
| E | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006520 | biological_process | amino acid metabolic process |
| F | 0008483 | molecular_function | transaminase activity |
| F | 0009058 | biological_process | biosynthetic process |
| F | 0016740 | molecular_function | transferase activity |
| F | 0030170 | molecular_function | pyridoxal phosphate binding |
| F | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| G | 0003824 | molecular_function | catalytic activity |
| G | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0006520 | biological_process | amino acid metabolic process |
| G | 0008483 | molecular_function | transaminase activity |
| G | 0009058 | biological_process | biosynthetic process |
| G | 0016740 | molecular_function | transferase activity |
| G | 0030170 | molecular_function | pyridoxal phosphate binding |
| G | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| H | 0003824 | molecular_function | catalytic activity |
| H | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0006520 | biological_process | amino acid metabolic process |
| H | 0008483 | molecular_function | transaminase activity |
| H | 0009058 | biological_process | biosynthetic process |
| H | 0016740 | molecular_function | transferase activity |
| H | 0030170 | molecular_function | pyridoxal phosphate binding |
| H | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE TRP A 414 |
| Chain | Residue |
| A | VAL16 |
| A | PLP413 |
| A | ASN20 |
| A | THR37 |
| A | ALA38 |
| A | GLY39 |
| A | GLU40 |
| A | ASN175 |
| A | TYR322 |
| A | ARG361 |
| site_id | AC2 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE TRP A 914 |
| Chain | Residue |
| A | TYR64 |
| A | THR265 |
| B | VAL516 |
| B | GLY539 |
| B | ASN675 |
| B | LYS734 |
| B | TYR822 |
| B | ARG861 |
| B | PLP913 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE TRP C 1414 |
| Chain | Residue |
| C | VAL1016 |
| C | ASN1020 |
| C | GLY1039 |
| C | LYS1234 |
| C | TYR1322 |
| C | ARG1361 |
| C | PLP1413 |
| D | TYR1564 |
| site_id | AC4 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE TRP D 1914 |
| Chain | Residue |
| C | TYR1064 |
| D | VAL1516 |
| D | ASN1520 |
| D | THR1537 |
| D | GLY1539 |
| D | ASN1675 |
| D | LYS1734 |
| D | TYR1822 |
| D | ARG1861 |
| D | PLP1913 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE TRP E 2414 |
| Chain | Residue |
| E | GLY2039 |
| E | TYR2322 |
| E | ARG2361 |
| E | PLP2413 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE TRP E 2914 |
| Chain | Residue |
| E | TYR2064 |
| F | VAL2516 |
| F | GLY2539 |
| F | LYS2734 |
| F | TYR2822 |
| F | ARG2861 |
| F | PLP2913 |
| site_id | AC7 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE TRP G 3414 |
| Chain | Residue |
| G | VAL3016 |
| G | ASN3020 |
| G | THR3037 |
| G | GLY3039 |
| G | GLU3040 |
| G | TRP3125 |
| G | ASN3175 |
| G | ARG3361 |
| G | PLP3413 |
| H | TYR3564 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE TRP H 3914 |
| Chain | Residue |
| H | VAL3516 |
| H | ASN3520 |
| H | THR3537 |
| H | GLY3539 |
| H | ASN3675 |
| H | TYR3822 |
| H | ARG3861 |
| H | PLP3913 |
| site_id | AC9 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PLP A 413 |
| Chain | Residue |
| A | GLY99 |
| A | GLY100 |
| A | SER101 |
| A | ASN171 |
| A | ASN175 |
| A | ASP203 |
| A | TYR206 |
| A | LYS234 |
| A | ARG242 |
| A | TYR322 |
| A | TRP414 |
| B | TYR564 |
| site_id | BC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE PLP B 913 |
| Chain | Residue |
| A | TYR64 |
| A | TRP914 |
| B | GLY600 |
| B | SER601 |
| B | TRP625 |
| B | ASN671 |
| B | ASN675 |
| B | ASP703 |
| B | TYR706 |
| B | LYS734 |
| B | ARG742 |
| site_id | BC2 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PLP C 1413 |
| Chain | Residue |
| C | TRP1414 |
| D | TYR1564 |
| C | GLY1100 |
| C | SER1101 |
| C | TRP1125 |
| C | ASN1175 |
| C | ASP1203 |
| C | TYR1206 |
| C | ALA1233 |
| C | LYS1234 |
| C | ARG1242 |
| C | TYR1322 |
| site_id | BC3 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PLP D 1913 |
| Chain | Residue |
| C | TYR1064 |
| D | GLY1600 |
| D | SER1601 |
| D | ASN1671 |
| D | ASN1675 |
| D | ASP1703 |
| D | TYR1706 |
| D | LYS1734 |
| D | ARG1742 |
| D | TRP1914 |
| site_id | BC4 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PLP E 2413 |
| Chain | Residue |
| E | GLY2099 |
| E | GLY2100 |
| E | SER2101 |
| E | TRP2125 |
| E | ASN2175 |
| E | ASP2203 |
| E | TYR2206 |
| E | ALA2233 |
| E | ARG2242 |
| E | TYR2322 |
| E | TRP2414 |
| F | TYR2564 |
| site_id | BC5 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PLP F 2913 |
| Chain | Residue |
| E | TYR2064 |
| E | TRP2914 |
| F | GLY2600 |
| F | SER2601 |
| F | TRP2625 |
| F | ASN2675 |
| F | ASP2703 |
| F | TYR2706 |
| F | LYS2734 |
| F | ARG2742 |
| site_id | BC6 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PLP G 3413 |
| Chain | Residue |
| G | GLY3100 |
| G | SER3101 |
| G | TRP3125 |
| G | ASN3171 |
| G | ASN3175 |
| G | ASP3203 |
| G | TYR3206 |
| G | ARG3242 |
| G | TRP3414 |
| H | TYR3564 |
| site_id | BC7 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PLP H 3913 |
| Chain | Residue |
| G | TYR3064 |
| H | GLY3599 |
| H | GLY3600 |
| H | SER3601 |
| H | TRP3625 |
| H | ASN3675 |
| H | ASP3703 |
| H | TYR3706 |
| H | LYS3734 |
| H | ARG3742 |
| H | TYR3822 |
| H | TRP3914 |
Functional Information from PROSITE/UniProt
| site_id | PS00105 |
| Number of Residues | 14 |
| Details | AA_TRANSFER_CLASS_1 Aminotransferases class-I pyridoxal-phosphate attachment site. GAAKafAMtGWRIG |
| Chain | Residue | Details |
| A | GLY231-GLY244 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P00509","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 24 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"1GCK","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | Site: {"description":"Important for prephenate aminotransferase activity","evidences":[{"source":"PubMed","id":"30771275","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"PubMed","id":"10029535","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11432784","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1B5O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1B5P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5BJ3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5BJ4","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | TYR124 | |
| A | ASP203 |
| site_id | CSA10 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | ALA568 |
| site_id | CSA11 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | ALA1068 |
| site_id | CSA12 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | ALA1568 |
| site_id | CSA13 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| E | ALA2068 |
| site_id | CSA14 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| F | ALA2568 |
| site_id | CSA15 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| G | ALA3068 |
| site_id | CSA16 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| H | ALA3568 |
| site_id | CSA17 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | LYS234 | |
| A | TRP125 | |
| A | ASP203 |
| site_id | CSA18 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | LYS734 | |
| B | TRP625 | |
| B | ASP703 |
| site_id | CSA19 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | ASP1203 | |
| C | LYS1234 | |
| C | TRP1125 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | TYR624 | |
| B | ASP703 |
| site_id | CSA20 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | ASP1703 | |
| D | LYS1734 | |
| D | TRP1625 |
| site_id | CSA21 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| E | TRP2125 | |
| E | ASP2203 | |
| E | LYS2234 |
| site_id | CSA22 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| F | TRP2625 | |
| F | ASP2703 | |
| F | LYS2734 |
| site_id | CSA23 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| G | TRP3125 | |
| G | ASP3203 | |
| G | LYS3234 |
| site_id | CSA24 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| H | TRP3625 | |
| H | ASP3703 | |
| H | LYS3734 |
| site_id | CSA25 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | LYS234 | |
| A | TYR124 | |
| A | ASP203 |
| site_id | CSA26 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | LYS734 | |
| B | TYR624 | |
| B | ASP703 |
| site_id | CSA27 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | TYR1124 | |
| C | ASP1203 | |
| C | LYS1234 |
| site_id | CSA28 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | ASP1703 | |
| D | TYR1624 | |
| D | LYS1734 |
| site_id | CSA29 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| E | TYR2124 | |
| E | ASP2203 | |
| E | LYS2234 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | TYR1124 | |
| C | ASP1203 |
| site_id | CSA30 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| F | ASP2703 | |
| F | TYR2624 | |
| F | LYS2734 |
| site_id | CSA31 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| G | TYR3124 | |
| G | ASP3203 | |
| G | LYS3234 |
| site_id | CSA32 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| H | ASP3703 | |
| H | LYS3734 | |
| H | TYR3624 |
| site_id | CSA33 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | TRP125 | |
| A | ASP203 |
| site_id | CSA34 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | TRP625 | |
| B | ASP703 |
| site_id | CSA35 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | ASP1203 | |
| C | TRP1125 |
| site_id | CSA36 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | ASP1703 | |
| D | TRP1625 |
| site_id | CSA37 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| E | TRP2125 | |
| E | ASP2203 |
| site_id | CSA38 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| F | TRP2625 | |
| F | ASP2703 |
| site_id | CSA39 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| G | TRP3125 | |
| G | ASP3203 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | ASP1703 | |
| D | TYR1624 |
| site_id | CSA40 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| H | TRP3625 | |
| H | ASP3703 |
| site_id | CSA41 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | LYS234 | |
| A | ASP203 | |
| A | TYR128 |
| site_id | CSA42 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | LYS734 | |
| B | TYR628 | |
| B | ASP703 |
| site_id | CSA43 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | ASP1203 | |
| C | LYS1234 | |
| C | TYR1128 |
| site_id | CSA44 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | TYR1628 | |
| D | ASP1703 | |
| D | LYS1734 |
| site_id | CSA45 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| E | TYR2128 | |
| E | ASP2203 | |
| E | LYS2234 |
| site_id | CSA46 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| F | ASP2703 | |
| F | TYR2628 | |
| F | LYS2734 |
| site_id | CSA47 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| G | TYR3128 | |
| G | ASP3203 | |
| G | LYS3234 |
| site_id | CSA48 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| H | TYR3628 | |
| H | ASP3703 | |
| H | LYS3734 |
| site_id | CSA5 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| E | TYR2124 | |
| E | ASP2203 |
| site_id | CSA6 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| F | ASP2703 | |
| F | TYR2624 |
| site_id | CSA7 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| G | TYR3124 | |
| G | ASP3203 |
| site_id | CSA8 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| H | ASP3703 | |
| H | TYR3624 |
| site_id | CSA9 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | ALA68 |
