1GA6

CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH A FRAGMENT OF TYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004252	molecular_function	serine-type endopeptidase activity
A	0006508	biological_process	proteolysis
A	0008236	molecular_function	serine-type peptidase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 374

Chain	Residue
A	ASP328
A	VAL329
A	GLY344
A	GLY346
A	ASP348
A	HOH401

---

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ACT A 395

Chain	Residue
A	GLY169
A	GLU171
A	ARG179
A	SER287
A	HOH486
A	LEU134
A	GLY135
A	SER167

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT A 396

Chain	Residue
A	THR24
A	PRO196
A	PRO244
A	HOH417
A	HOH461
A	HOH480

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT A 397

Chain	Residue
A	LYS128
A	GLY161
A	SER307
A	LEU308
A	GLY309
A	GOL392
A	HOH635

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT A 398

Chain	Residue
A	SER324
A	VAL326
A	HIS327
A	ASP357
A	LYS360

---

site_id	AC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GOL A 391

Chain	Residue
A	ASN9
A	PHE48
A	ASN52
A	THR186
A	LEU273
A	TYR275
A	LEU280
A	HOH434
A	HOH489
A	HOH515
A	HOH564
A	HOH866

---

site_id	AC7
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL A 392

Chain	Residue
A	SER20
A	GLY309
A	SER314
A	PHE370
A	ACT397
A	HOH414
A	HOH424
A	HOH437
A	HOH444
A	HOH459
A	HOH460

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 393

Chain	Residue
A	ASN125
A	VAL126
A	ASN306
A	HOH465
A	HOH484
A	HOH680
A	HOH751

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 394

Chain	Residue
A	ALA5
A	ASN221
A	GLU222
A	HOH421
A	HOH651

---

Functional Information from PROSITE/UniProt

site_id	PS00138
Number of Residues	11
Details	SUBTILASE_SER Serine proteases, subtilase family, serine active site. GTSlAsPiFVG

Chain	Residue	Details
A	GLY285-GLY295

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	364
Details	Domain: {"description":"Peptidase S53","evidences":[{"source":"PROSITE-ProRule","id":"PRU01032","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	19
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"11323721","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11747435","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11323721","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11747435","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1GA4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GA6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1NLU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6M8W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6M8Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6M9C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6M9D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6M9F","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1nlu

Chain	Residue	Details
A	GLU80
A	ASP170
A	SER287
A	ASP84

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 380

Chain	Residue	Details
A	GLU80	proton shuttle (general acid/base)
A	ASP84	proton shuttle (general acid/base)
A	ASP170	electrostatic stabiliser
A	SER287	covalent catalysis

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