1GA4

CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH INHIBITOR PSEUDOIODOTYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004252	molecular_function	serine-type endopeptidase activity
A	0006508	biological_process	proteolysis
A	0008236	molecular_function	serine-type peptidase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 374

Chain	Residue
A	ASP328
A	VAL329
A	GLY344
A	GLY346
A	ASP348
A	HOH401

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site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 391

Chain	Residue
A	THR186
A	TYR275
A	LEU280
A	HOH434
A	HOH489
A	HOH564
A	ASN9
A	PHE48
A	ASN52

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site_id	AC3
Number of Residues	20
Details	BINDING SITE FOR CHAIN I OF PSEUDOIODOTYROSTATIN

Chain	Residue
A	ILE35
A	ASP74
A	GLY77
A	GLU80
A	TRP81
A	SER133
A	LEU134
A	GLY135
A	TRP136
A	SER167
A	GLY169
A	ASP170
A	GLU171
A	GLU175
A	ARG179
A	SER190
A	GLY285
A	THR286
A	SER287
A	HOH637

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Functional Information from PROSITE/UniProt

site_id	PS00138
Number of Residues	11
Details	SUBTILASE_SER Serine proteases, subtilase family, serine active site. GTSlAsPiFVG

Chain	Residue	Details
A	GLY285-GLY295

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: Charge relay system => ECO:0000269|PubMed:10488127

Chain	Residue	Details
A	GLU80
A	ASP84
A	SER287

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site_id	SWS_FT_FI2
Number of Residues	5
Details	BINDING:

Chain	Residue	Details
A	ASP328
A	VAL329
A	GLY344
A	GLY346
A	ASP348

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1nlu

Chain	Residue	Details
A	GLU80
A	ASP170
A	SER287
A	ASP84

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site_id	MCSA1
Number of Residues	4
Details	M-CSA 380

Chain	Residue	Details
A	GLU80	proton shuttle (general acid/base)
A	ASP84	proton shuttle (general acid/base)
A	ASP170	electrostatic stabiliser
A	SER287	covalent catalysis

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