1G8T
SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT 1.1 A RESOLUTION
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000014 | molecular_function | single-stranded DNA endodeoxyribonuclease activity |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004521 | molecular_function | RNA endonuclease activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000014 | molecular_function | single-stranded DNA endodeoxyribonuclease activity |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004521 | molecular_function | RNA endonuclease activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0006259 | biological_process | DNA metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A 801 |
| Chain | Residue |
| A | ARG57 |
| A | ASN119 |
| A | HOH939 |
| A | HOH1136 |
| A | HOH1163 |
| A | HOH1246 |
| A | HOH1248 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE SO4 B 802 |
| Chain | Residue |
| B | ARG87 |
| B | HIS89 |
| B | ASN119 |
| B | HOH948 |
| B | HOH1138 |
| B | HOH1139 |
| B | HOH1189 |
| B | HOH1216 |
| B | HOH1219 |
| A | ASN20 |
| B | ARG57 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 B 803 |
| Chain | Residue |
| A | HOH914 |
| A | HOH938 |
| B | THR77 |
| B | GLY78 |
| B | ARG87 |
| B | HOH861 |
| B | HOH870 |
| B | HOH1107 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 A 804 |
| Chain | Residue |
| A | ARG25 |
| A | LYS84 |
| A | TYR107 |
| A | HOH905 |
| A | HOH964 |
| A | HOH1153 |
| A | HOH1174 |
| A | HOH1233 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE MG A 810 |
| Chain | Residue |
| A | ASN119 |
| A | HOH1163 |
| A | HOH1164 |
| A | HOH1246 |
| A | HOH1247 |
| A | HOH1248 |
| A | HOH1249 |
| A | HOH1250 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 811 |
| Chain | Residue |
| B | ASN119 |
| B | HOH1216 |
| B | HOH1217 |
| B | HOH1218 |
| B | HOH1219 |
| B | HOH1220 |
Functional Information from PROSITE/UniProt
| site_id | PS01070 |
| Number of Residues | 9 |
| Details | NUCLEASE_NON_SPEC DNA/RNA non-specific endonucleases active site. DRGHQaplA |
| Chain | Residue | Details |
| A | ASP86-ALA94 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10047, ECO:0000269|PubMed:8078761 |
| Chain | Residue | Details |
| A | ASN110 | |
| B | ASN110 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | SER140 | |
| B | SER140 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 42 |
| Chain | Residue | Details |
| A | GLY78 | electrostatic stabiliser |
| A | ASN110 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | ARG131 | electrostatic stabiliser, increase acidity, increase basicity |
| A | SER140 | activator, hydrogen bond acceptor, hydrogen bond donor, metal ligand |
| A | PRO148 | electrostatic stabiliser, hydrogen bond acceptor, metal ligand, proton acceptor, proton donor |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 42 |
| Chain | Residue | Details |
| B | ARG131 | electrostatic stabiliser, increase acidity, increase basicity |
| B | SER140 | activator, hydrogen bond acceptor, hydrogen bond donor, metal ligand |
| B | PRO148 | electrostatic stabiliser, hydrogen bond acceptor, metal ligand, proton acceptor, proton donor |
| B | GLY78 | electrostatic stabiliser |
| B | ASN110 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
