1G4B

CRYSTAL STRUCTURES OF THE HSLVU PEPTIDASE-ATPASE COMPLEX REVEAL AN ATP-DEPENDENT PROTEOLYSIS MECHANISM

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0000287	molecular_function	magnesium ion binding
E	0004176	molecular_function	ATP-dependent peptidase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006508	biological_process	proteolysis
E	0008233	molecular_function	peptidase activity
E	0009376	cellular_component	HslUV protease complex
E	0009408	biological_process	response to heat
E	0016020	cellular_component	membrane
E	0016887	molecular_function	ATP hydrolysis activity
E	0019904	molecular_function	protein domain specific binding
E	0030164	biological_process	protein denaturation
E	0034605	biological_process	cellular response to heat
E	0036402	molecular_function	proteasome-activating activity
E	0042802	molecular_function	identical protein binding
E	0043335	biological_process	protein unfolding
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	1901800	biological_process	positive regulation of proteasomal protein catabolic process
F	0000287	molecular_function	magnesium ion binding
F	0004176	molecular_function	ATP-dependent peptidase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006508	biological_process	proteolysis
F	0008233	molecular_function	peptidase activity
F	0009376	cellular_component	HslUV protease complex
F	0009408	biological_process	response to heat
F	0016020	cellular_component	membrane
F	0016887	molecular_function	ATP hydrolysis activity
F	0019904	molecular_function	protein domain specific binding
F	0030164	biological_process	protein denaturation
F	0034605	biological_process	cellular response to heat
F	0036402	molecular_function	proteasome-activating activity
F	0042802	molecular_function	identical protein binding
F	0043335	biological_process	protein unfolding
F	0051603	biological_process	proteolysis involved in protein catabolic process
F	1901800	biological_process	positive regulation of proteasomal protein catabolic process
K	0000287	molecular_function	magnesium ion binding
K	0004176	molecular_function	ATP-dependent peptidase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006508	biological_process	proteolysis
K	0008233	molecular_function	peptidase activity
K	0009376	cellular_component	HslUV protease complex
K	0009408	biological_process	response to heat
K	0016020	cellular_component	membrane
K	0016887	molecular_function	ATP hydrolysis activity
K	0019904	molecular_function	protein domain specific binding
K	0030164	biological_process	protein denaturation
K	0034605	biological_process	cellular response to heat
K	0036402	molecular_function	proteasome-activating activity
K	0042802	molecular_function	identical protein binding
K	0043335	biological_process	protein unfolding
K	0051603	biological_process	proteolysis involved in protein catabolic process
K	1901800	biological_process	positive regulation of proteasomal protein catabolic process
L	0000287	molecular_function	magnesium ion binding
L	0004176	molecular_function	ATP-dependent peptidase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006508	biological_process	proteolysis
L	0008233	molecular_function	peptidase activity
L	0009376	cellular_component	HslUV protease complex
L	0009408	biological_process	response to heat
L	0016020	cellular_component	membrane
L	0016887	molecular_function	ATP hydrolysis activity
L	0019904	molecular_function	protein domain specific binding
L	0030164	biological_process	protein denaturation
L	0034605	biological_process	cellular response to heat
L	0036402	molecular_function	proteasome-activating activity
L	0042802	molecular_function	identical protein binding
L	0043335	biological_process	protein unfolding
L	0051603	biological_process	proteolysis involved in protein catabolic process
L	1901800	biological_process	positive regulation of proteasomal protein catabolic process
M	0000287	molecular_function	magnesium ion binding
M	0004176	molecular_function	ATP-dependent peptidase activity
M	0004298	molecular_function	threonine-type endopeptidase activity
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005839	cellular_component	proteasome core complex
M	0006508	biological_process	proteolysis
M	0008233	molecular_function	peptidase activity
M	0009376	cellular_component	HslUV protease complex
M	0009408	biological_process	response to heat
M	0019904	molecular_function	protein domain specific binding
M	0030163	biological_process	protein catabolic process
M	0030164	biological_process	protein denaturation
M	0034605	biological_process	cellular response to heat
M	0042802	molecular_function	identical protein binding
M	0046872	molecular_function	metal ion binding
M	0051603	biological_process	proteolysis involved in protein catabolic process
N	0000287	molecular_function	magnesium ion binding
N	0004176	molecular_function	ATP-dependent peptidase activity
N	0004298	molecular_function	threonine-type endopeptidase activity
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005839	cellular_component	proteasome core complex
N	0006508	biological_process	proteolysis
N	0008233	molecular_function	peptidase activity
N	0009376	cellular_component	HslUV protease complex
N	0009408	biological_process	response to heat
N	0019904	molecular_function	protein domain specific binding
N	0030163	biological_process	protein catabolic process
N	0030164	biological_process	protein denaturation
N	0034605	biological_process	cellular response to heat
N	0042802	molecular_function	identical protein binding
N	0046872	molecular_function	metal ion binding
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0000287	molecular_function	magnesium ion binding
O	0004176	molecular_function	ATP-dependent peptidase activity
O	0004298	molecular_function	threonine-type endopeptidase activity
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005839	cellular_component	proteasome core complex
O	0006508	biological_process	proteolysis
O	0008233	molecular_function	peptidase activity
O	0009376	cellular_component	HslUV protease complex
O	0009408	biological_process	response to heat
O	0019904	molecular_function	protein domain specific binding
O	0030163	biological_process	protein catabolic process
O	0030164	biological_process	protein denaturation
O	0034605	biological_process	cellular response to heat
O	0042802	molecular_function	identical protein binding
O	0046872	molecular_function	metal ion binding
O	0051603	biological_process	proteolysis involved in protein catabolic process
P	0000287	molecular_function	magnesium ion binding
P	0004176	molecular_function	ATP-dependent peptidase activity
P	0004298	molecular_function	threonine-type endopeptidase activity
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005839	cellular_component	proteasome core complex
P	0006508	biological_process	proteolysis
P	0008233	molecular_function	peptidase activity
P	0009376	cellular_component	HslUV protease complex
P	0009408	biological_process	response to heat
P	0019904	molecular_function	protein domain specific binding
P	0030163	biological_process	protein catabolic process
P	0030164	biological_process	protein denaturation
P	0034605	biological_process	cellular response to heat
P	0042802	molecular_function	identical protein binding
P	0046872	molecular_function	metal ion binding
P	0051603	biological_process	proteolysis involved in protein catabolic process

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: ACT_SITE => ECO:0000255|HAMAP-Rule:MF_00248, ECO:0000269|PubMed:9257689

Chain	Residue	Details
M	THR2
F	ARG393
K	ILE18
K	GLY60
K	ASP256
K	GLU321
K	ARG393
L	ILE18
L	GLY60
L	ASP256
L	GLU321
N	THR2
L	ARG393
O	THR2
P	THR2
E	ARG393
F	ILE18
F	GLY60
F	ASP256
F	GLU321

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING:

Chain	Residue	Details
M	ASP157
P	ASP157
P	ILE160
P	ASN163
M	ILE160
M	ASN163
N	ASP157
N	ILE160
N	ASN163
O	ASP157
O	ILE160
O	ASN163

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
M	GLY48
M	LYS33
M	THR1
M	SER124

---

site_id	CSA10
Number of Residues	3
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
F	LYS140
F	LYS240
F	LYS63

---

site_id	CSA11
Number of Residues	3
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
K	LYS140
K	LYS240
K	LYS63

---

site_id	CSA12
Number of Residues	3
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
L	LYS140
L	LYS240
L	LYS63

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
N	GLY48
N	LYS33
N	THR1
N	SER124

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
O	GLY48
O	LYS33
O	THR1
O	SER124

---

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
P	GLY48
P	LYS33
P	THR1
P	SER124

---

site_id	CSA5
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
M	GLY45
M	LYS33
M	THR1
M	SER124

---

site_id	CSA6
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
N	GLY45
N	LYS33
N	THR1
N	SER124

---

site_id	CSA7
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
O	GLY45
O	LYS33
O	THR1
O	SER124

---

site_id	CSA8
Number of Residues	4
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
P	GLY45
P	LYS33
P	THR1
P	SER124

---

site_id	CSA9
Number of Residues	3
Details	Annotated By Reference To The Literature 1pma

Chain	Residue	Details
E	LYS140
E	LYS240
E	LYS63

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
M	THR2	proton acceptor, proton donor
M	VAL34	electrostatic stabiliser
M	PHE46	electrostatic stabiliser
M	GLY125	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
N	THR2	proton acceptor, proton donor
N	VAL34	electrostatic stabiliser
N	PHE46	electrostatic stabiliser
N	GLY125	electrostatic stabiliser

---

site_id	MCSA3
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
O	THR2	proton acceptor, proton donor
O	VAL34	electrostatic stabiliser
O	PHE46	electrostatic stabiliser
O	GLY125	electrostatic stabiliser

---

site_id	MCSA4
Number of Residues	4
Details	M-CSA 678

Chain	Residue	Details
P	THR2	proton acceptor, proton donor
P	VAL34	electrostatic stabiliser
P	PHE46	electrostatic stabiliser
P	GLY125	electrostatic stabiliser

---