1FWI
KLEBSIELLA AEROGENES UREASE, H134A VARIANT
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0009039 | molecular_function | urease activity |
| A | 0016151 | molecular_function | nickel cation binding |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019627 | biological_process | urea metabolic process |
| A | 0043419 | biological_process | urea catabolic process |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0009039 | molecular_function | urease activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0035550 | cellular_component | urease complex |
| B | 0043419 | biological_process | urea catabolic process |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0009039 | molecular_function | urease activity |
| C | 0016151 | molecular_function | nickel cation binding |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0016810 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds |
| C | 0043419 | biological_process | urea catabolic process |
| C | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NI C 574 |
| Chain | Residue |
| C | HIS246 |
| C | HIS272 |
| C | HOH723 |
| C | HOH724 |
| C | HOH726 |
| C | KCX217 |
| site_id | ACT |
| Number of Residues | 1 |
| Details | RESIDUE IMPLICATED IN CATALYSIS. |
| Chain | Residue |
| C | HIS219 |
| site_id | NIL |
| Number of Residues | 7 |
| Details | NICKEL METALLOCENTER. |
| Chain | Residue |
| C | NI574 |
| C | KCX217 |
| C | HIS246 |
| C | HIS272 |
| C | HOH723 |
| C | HOH724 |
| C | HOH726 |
Functional Information from PROSITE/UniProt
| site_id | PS00145 |
| Number of Residues | 17 |
| Details | UREASE_2 Urease active site. MVCHHLdpdIaeDVaFA |
| Chain | Residue | Details |
| C | MET317-ALA333 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Binding site: {"description":"via carbamate group"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-carboxylysine","evidences":[{"source":"HAMAP-Rule","id":"MF_01953","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10913264","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7754395","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8702515","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8718850","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1kra |
| Chain | Residue | Details |
| C | ASP221 | |
| C | ARG336 | |
| C | HIS219 |
| site_id | MCSA1 |
| Number of Residues | 9 |
| Details | M-CSA 87 |
| Chain | Residue | Details |
| C | ALA134 | metal ligand |
| C | HIS136 | metal ligand |
| C | KCX217 | activator, metal ligand |
| C | GLY223 | proton acceptor, proton donor, proton relay |
| C | THR225 | activator, proton acceptor, proton donor |
| C | LEU250 | metal ligand |
| C | ALA276 | metal ligand |
| C | GLU340 | activator |
| C | MET364 | activator, metal ligand |
