Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1F76

ESCHERICHIA COLI DIHYDROOROTATE DEHYDROGENASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004152	molecular_function	dihydroorotate dehydrogenase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
A	0006221	biological_process	pyrimidine nucleotide biosynthetic process
A	0006222	biological_process	UMP biosynthetic process
A	0009220	biological_process	pyrimidine ribonucleotide biosynthetic process
A	0010181	molecular_function	FMN binding
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0044205	biological_process	'de novo' UMP biosynthetic process
A	0106430	molecular_function	dihydroorotate dehydrogenase (quinone) activity
B	0004152	molecular_function	dihydroorotate dehydrogenase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
B	0006221	biological_process	pyrimidine nucleotide biosynthetic process
B	0006222	biological_process	UMP biosynthetic process
B	0009220	biological_process	pyrimidine ribonucleotide biosynthetic process
B	0010181	molecular_function	FMN binding
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
B	0044205	biological_process	'de novo' UMP biosynthetic process
B	0106430	molecular_function	dihydroorotate dehydrogenase (quinone) activity
D	0004152	molecular_function	dihydroorotate dehydrogenase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
D	0006221	biological_process	pyrimidine nucleotide biosynthetic process
D	0006222	biological_process	UMP biosynthetic process
D	0009220	biological_process	pyrimidine ribonucleotide biosynthetic process
D	0010181	molecular_function	FMN binding
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
D	0044205	biological_process	'de novo' UMP biosynthetic process
D	0106430	molecular_function	dihydroorotate dehydrogenase (quinone) activity
E	0004152	molecular_function	dihydroorotate dehydrogenase activity
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
E	0006221	biological_process	pyrimidine nucleotide biosynthetic process
E	0006222	biological_process	UMP biosynthetic process
E	0009220	biological_process	pyrimidine ribonucleotide biosynthetic process
E	0010181	molecular_function	FMN binding
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
E	0044205	biological_process	'de novo' UMP biosynthetic process
E	0106430	molecular_function	dihydroorotate dehydrogenase (quinone) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE FMN A 1337

Chain	Residue
A	ALA61
A	THR245
A	THR247
A	SER267
A	GLY268
A	LEU271
A	VAL295
A	GLY296
A	GLY297
A	TYR318
A	SER319
A	ALA62
A	ORO1338
A	HOH1352
A	HOH1404
A	HOH1675
A	GLY63
A	LYS66
A	THR86
A	ASN111
A	ASN139
A	ASN172
A	LYS217

site_id	AC2
Number of Residues	11
Details	BINDING SITE FOR RESIDUE ORO A 1338

Chain	Residue
A	LYS66
A	ASN111
A	MSE113
A	GLY114
A	PHE115
A	ASN172
A	SER175
A	ASN177
A	ASN246
A	THR247
A	FMN1337

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE ORO A 1339

Chain	Residue
A	MSE1
A	LEU37
A	VAL38
A	ARG39
A	GLN40
A	PRO326
A	HOH1517
A	HOH1528
A	HOH1677

site_id	AC4
Number of Residues	23
Details	BINDING SITE FOR RESIDUE FMN B 1437

Chain	Residue
B	ALA61
B	ALA62
B	GLY63
B	LYS66
B	THR86
B	ASN111
B	ASN139
B	ASN172
B	LYS217
B	THR245
B	ASN246
B	THR247
B	SER267
B	GLY268
B	VAL295
B	GLY296
B	GLY297
B	TYR318
B	SER319
B	FMT1439
B	FMT1440
B	HOH1441
B	HOH1463

site_id	AC5
Number of Residues	24
Details	BINDING SITE FOR RESIDUE FMN D 1537

Chain	Residue
D	ALA61
D	ALA62
D	GLY63
D	LYS66
D	THR86
D	ASN111
D	ASN139
D	ASN172
D	LYS217
D	THR245
D	ASN246
D	THR247
D	SER267
D	GLY268
D	VAL295
D	GLY296
D	GLY297
D	TYR318
D	SER319
D	FMT1538
D	FMT1539
D	HOH1542
D	HOH1561
D	HOH1564

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ORO D 1540

Chain	Residue
D	MSE1
D	LEU37
D	VAL38
D	GLN40
D	PRO326
D	HOH1617

site_id	AC7
Number of Residues	26
Details	BINDING SITE FOR RESIDUE FMN E 1637

Chain	Residue
E	LYS66
E	THR86
E	LEU100
E	ASN111
E	ASN139
E	ASN172
E	LYS217
E	THR245
E	ASN246
E	THR247
E	SER267
E	GLY268
E	LEU271
E	VAL295
E	GLY296
E	GLY297
E	TYR318
E	SER319
E	FMT1638
E	FMT1640
E	HOH1641
E	HOH1653
E	HOH1693
E	ALA61
E	ALA62
E	GLY63

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT B 1438

Chain	Residue
B	ASN111
B	ASN177
B	ASN246
B	THR247
B	FMT1439
B	FMT1440

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE FMT B 1439

Chain	Residue
B	PHE115
B	ASN172
B	SER175
B	ASN246
B	FMN1437
B	FMT1438
B	FMT1440

site_id	BC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE FMT B 1440

Chain	Residue
B	LYS66
B	MSE113
B	GLY114
B	PHE115
B	SER175
B	ASN177
B	FMN1437
B	FMT1438
B	FMT1439

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT D 1538

Chain	Residue
D	ASN172
D	SER175
D	ASN246
D	FMN1537
D	FMT1539

site_id	BC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE FMT D 1539

Chain	Residue
D	LYS66
D	MSE113
D	GLY114
D	PHE115
D	SER175
D	ASN177
D	FMN1537
D	FMT1538
D	HOH1557

site_id	BC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE FMT E 1638

Chain	Residue
E	LYS66
E	MSE113
E	GLY114
E	PHE115
E	ASN177
E	FMN1637
E	FMT1639
E	FMT1640

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT E 1639

Chain	Residue
E	ASN111
E	ASN177
E	THR247
E	GLY265
E	FMT1638
E	FMT1640

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE FMT E 1640

Chain	Residue
E	ASN172
E	SER175
E	PRO176
E	ASN246
E	FMN1637
E	FMT1638
E	FMT1639

Functional Information from PROSITE/UniProt

site_id	PS00911
Number of Residues	20
Details	DHODEHASE_1 Dihydroorotate dehydrogenase signature 1. GsieiGTVTprpQpGNdkPR

Chain	Residue	Details
A	GLY80-ARG99

site_id	PS00912
Number of Residues	21
Details	DHODEHASE_2 Dihydroorotate dehydrogenase signature 2. IIGvGGIdSviaAreKIaAGA

Chain	Residue	Details
A	ILE292-ALA312

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
A	SER175
B	SER175
D	SER175
E	SER175

site_id	SWS_FT_FI2
Number of Residues	32
Details	BINDING: BINDING => ECO:0000269\|PubMed:12220493

Chain	Residue	Details
A	ALA62
B	THR86
B	ASN139
B	LYS217
B	THR245
B	GLY268
B	GLY297
B	TYR318
D	ALA62
D	THR86
D	ASN139
A	THR86
D	LYS217
D	THR245
D	GLY268
D	GLY297
D	TYR318
E	ALA62
E	THR86
E	ASN139
E	LYS217
E	THR245
A	ASN139
E	GLY268
E	GLY297
E	TYR318
A	LYS217
A	THR245
A	GLY268
A	GLY297
A	TYR318
B	ALA62

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING:

Chain	Residue	Details
A	LYS66
B	ASN246
D	LYS66
D	ASN111
D	ASN172
D	ASN177
D	ASN246
E	LYS66
E	ASN111
E	ASN172
E	ASN177
A	ASN111
E	ASN246
A	ASN172
A	ASN177
A	ASN246
B	LYS66
B	ASN111
B	ASN172
B	ASN177

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1h7x

Chain	Residue	Details
A	SER175
A	THR178
A	PHE115
A	LYS217

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1h7x

Chain	Residue	Details
B	SER175
B	THR178
B	PHE115
B	LYS217

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1h7x

Chain	Residue	Details
D	SER175
D	THR178
D	PHE115
D	LYS217

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 1h7x

Chain	Residue	Details
E	SER175
E	THR178
E	PHE115
E	LYS217

site_id	CSA5
Number of Residues	1
Details	Annotated By Reference To The Literature 1h7x

Chain	Residue	Details
A	SER175

site_id	CSA6
Number of Residues	1
Details	Annotated By Reference To The Literature 1h7x

Chain	Residue	Details
B	SER175

site_id	CSA7
Number of Residues	1
Details	Annotated By Reference To The Literature 1h7x

Chain	Residue	Details
D	SER175

site_id	CSA8
Number of Residues	1
Details	Annotated By Reference To The Literature 1h7x

Chain	Residue	Details
E	SER175

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)