1F74

CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM II COMPLEXED WITH 4-DEOXY-SIALIC ACID

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005975	biological_process	carbohydrate metabolic process
A	0008747	molecular_function	N-acetylneuraminate lyase activity
A	0016829	molecular_function	lyase activity
A	0019262	biological_process	N-acetylneuraminate catabolic process
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005975	biological_process	carbohydrate metabolic process
C	0008747	molecular_function	N-acetylneuraminate lyase activity
C	0016829	molecular_function	lyase activity
C	0019262	biological_process	N-acetylneuraminate catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL C 999

Chain	Residue
C	TYR180
C	PRO181
C	ASN182
C	HIS183

---

site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE NAY A 950

Chain	Residue
A	LYS164
A	GLY188
A	PHE189
A	ASP190
A	GLU191
A	ILE205
A	GLY206
A	SER207
A	LEU250
A	HOH1312
A	HOH1313
A	HOH1344
A	HOH1373
A	ALA10
A	TYR43
A	SER47
A	THR48
A	TYR136

---

site_id	AC3
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NAY C 951

Chain	Residue
C	ALA10
C	TYR43
C	SER47
C	THR48
C	TYR136
C	LYS164
C	GLY188
C	PHE189
C	ASP190
C	GLU191
C	ILE205
C	GLY206
C	SER207
C	LEU250
C	HOH1260
C	HOH1290
C	HOH1296
C	HOH1315
C	HOH1329

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL C 931

Chain	Residue
C	ASN132
C	PRO158
C	LYS159
C	LEU161
C	GLU230
C	GOL932
C	HOH1141
C	HOH1176
C	HOH1262

---

site_id	AC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL C 932

Chain	Residue
C	PRO158
C	VAL160
C	LEU161
C	ASN182
C	VAL214
C	GLN218
C	GOL931
C	HOH1153
C	HOH1154
C	HOH1343
C	HOH1371

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 933

Chain	Residue
A	ARG2
A	LYS67
A	ASP72
A	ASP101
A	ALA289
A	SER293
A	HOH977
A	HOH1240

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 934

Chain	Residue
A	GLY226
A	LYS227
A	LEU228
A	LYS229
A	GLU230
A	HOH1232

---

Functional Information from PROSITE/UniProt

site_id	PS00217
Number of Residues	26
Details	SUGAR_TRANSPORT_2 Sugar transport proteins signature 2. VdGLYvGGstgenfmLstEekkeifR

Chain	Residue	Details
A	VAL39-ARG64

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MRDL

Chain	Residue	Details
A	MET1-LEU4

---

site_id	PS00665
Number of Residues	18
Details	DHDPS_1 Dihydrodipicolinate synthase signature 1. GLYvgGSTGENfmlsteE

Chain	Residue	Details
A	GLY41-GLU58

---

site_id	PS00666
Number of Residues	31
Details	DHDPS_2 Dihydrodipicolinate synthase signature 2. YSIPflTgvnMgieqfgelyknpk.VlGVKFT

Chain	Residue	Details
A	TYR136-THR166

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_01237, ECO:0000305|PubMed:11031117

Chain	Residue	Details
A	TYR136
C	TYR136

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Schiff-base intermediate with substrate => ECO:0000255|HAMAP-Rule:MF_01237, ECO:0000305|PubMed:11031117

Chain	Residue	Details
A	LYS164
C	LYS164

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01237

Chain	Residue	Details
A	SER47
C	THR166
C	GLY188
C	ASP190
C	GLU191
C	SER207
A	THR48
A	THR166
A	GLY188
A	ASP190
A	GLU191
A	SER207
C	SER47
C	THR48

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1dhp

Chain	Residue	Details
A	TYR136
A	LYS164
A	LEU141

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1dhp

Chain	Residue	Details
C	TYR136
C	LYS164
C	LEU141

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1dhp

Chain	Residue	Details
A	THR48
A	LYS164
A	SER47

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1dhp

Chain	Residue	Details
C	THR48
C	LYS164
C	SER47

---