1F61
CRYSTAL STRUCTURE OF ISOCITRATE LYASE FROM MYCOBACTERIUM TUBERCULOSIS
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004451 | molecular_function | isocitrate lyase activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006097 | biological_process | glyoxylate cycle |
| A | 0006099 | biological_process | tricarboxylic acid cycle |
| A | 0006102 | biological_process | isocitrate metabolic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| A | 0035375 | molecular_function | zymogen binding |
| A | 0046421 | molecular_function | methylisocitrate lyase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0052572 | biological_process | response to host immune response |
| A | 0071456 | biological_process | cellular response to hypoxia |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004451 | molecular_function | isocitrate lyase activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006097 | biological_process | glyoxylate cycle |
| B | 0006099 | biological_process | tricarboxylic acid cycle |
| B | 0006102 | biological_process | isocitrate metabolic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0035375 | molecular_function | zymogen binding |
| B | 0046421 | molecular_function | methylisocitrate lyase activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0052572 | biological_process | response to host immune response |
| B | 0071456 | biological_process | cellular response to hypoxia |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 451 |
| Chain | Residue |
| A | ASP108 |
| A | GLU155 |
| A | HOH575 |
| A | HOH690 |
| A | HOH699 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 452 |
| Chain | Residue |
| B | HOH705 |
| B | ASP108 |
| B | GLU155 |
| B | HOH595 |
| B | HOH635 |
Functional Information from PROSITE/UniProt
| site_id | PS00161 |
| Number of Residues | 6 |
| Details | ISOCITRATE_LYASE Isocitrate lyase signature. KKCGHL |
| Chain | Residue | Details |
| A | LYS189-LEU194 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000305|PubMed:24354272 |
| Chain | Residue | Details |
| A | LYS190 | |
| B | LYS190 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10932251 |
| Chain | Residue | Details |
| A | LEU90 | |
| B | ALA227 | |
| B | TYR312 | |
| B | ILE346 | |
| A | ALA152 | |
| A | CYS191 | |
| A | ALA227 | |
| A | TYR312 | |
| A | ILE346 | |
| B | LEU90 | |
| B | ALA152 | |
| B | CYS191 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | CROSSLNK: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup) => ECO:0000269|PubMed:20066036 |
| Chain | Residue | Details |
| A | GLN333 | |
| B | GLN333 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1f8m |
| Chain | Residue | Details |
| A | ARG228 | |
| A | HIS180 | |
| A | CYS191 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1f8m |
| Chain | Residue | Details |
| B | ARG228 | |
| B | HIS180 | |
| B | CYS191 |
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 272 |
| Chain | Residue | Details |
| A | ALA152 | metal ligand |
| A | SER179 | electrostatic stabiliser, hydrogen bond donor |
| A | LYS190 | covalent catalysis, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, proton shuttle (general acid/base) |
| A | GLY192 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton shuttle (general acid/base) |
| A | ALA227 | electrostatic stabiliser, hydrogen bond donor |
| A | CYS314 | electrostatic stabiliser, hydrogen bond donor |
| A | PRO316 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 7 |
| Details | M-CSA 272 |
| Chain | Residue | Details |
| B | ALA152 | metal ligand |
| B | SER179 | electrostatic stabiliser, hydrogen bond donor |
| B | LYS190 | covalent catalysis, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, proton shuttle (general acid/base) |
| B | GLY192 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton shuttle (general acid/base) |
| B | ALA227 | electrostatic stabiliser, hydrogen bond donor |
| B | CYS314 | electrostatic stabiliser, hydrogen bond donor |
| B | PRO316 | electrostatic stabiliser, hydrogen bond donor |
