1EZ4
CRYSTAL STRUCTURE OF NON-ALLOSTERIC L-LACTATE DEHYDROGENASE FROM LACTOBACILLUS PENTOSUS AT 2.3 ANGSTROM RESOLUTION
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006089 | biological_process | lactate metabolic process |
| A | 0006090 | biological_process | pyruvate metabolic process |
| A | 0006096 | biological_process | glycolytic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006089 | biological_process | lactate metabolic process |
| B | 0006090 | biological_process | pyruvate metabolic process |
| B | 0006096 | biological_process | glycolytic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006089 | biological_process | lactate metabolic process |
| C | 0006090 | biological_process | pyruvate metabolic process |
| C | 0006096 | biological_process | glycolytic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0019752 | biological_process | carboxylic acid metabolic process |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004459 | molecular_function | L-lactate dehydrogenase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006089 | biological_process | lactate metabolic process |
| D | 0006090 | biological_process | pyruvate metabolic process |
| D | 0006096 | biological_process | glycolytic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0019752 | biological_process | carboxylic acid metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE NAD A 1352 |
| Chain | Residue |
| A | GLY29 |
| A | GLY97 |
| A | ALA98 |
| A | ILE116 |
| A | SER119 |
| A | ALA136 |
| A | ASN138 |
| A | VAL140 |
| A | SER161 |
| A | LEU165 |
| A | HIS193 |
| A | ALA30 |
| A | HOH1427 |
| A | HOH1476 |
| A | HOH1527 |
| A | VAL31 |
| A | ASP52 |
| A | VAL53 |
| A | ARG57 |
| A | TYR83 |
| A | THR95 |
| A | ALA96 |
| site_id | AC2 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE NAD B 1353 |
| Chain | Residue |
| B | ASP28 |
| B | GLY29 |
| B | ALA30 |
| B | VAL31 |
| B | ASP52 |
| B | VAL54 |
| B | THR95 |
| B | ALA96 |
| B | GLY97 |
| B | ILE116 |
| B | ALA136 |
| B | ALA137 |
| B | ASN138 |
| B | VAL140 |
| B | SER161 |
| B | LEU165 |
| B | HIS193 |
| B | ILE251 |
| B | HOH1457 |
| B | HOH1458 |
| B | HOH1471 |
| B | HOH1472 |
| B | HOH1544 |
| B | HOH1557 |
| site_id | AC3 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE NAD C 1354 |
| Chain | Residue |
| C | ASP28 |
| C | GLY29 |
| C | ALA30 |
| C | VAL31 |
| C | ASP52 |
| C | VAL53 |
| C | ARG57 |
| C | TYR83 |
| C | THR95 |
| C | ALA96 |
| C | GLY97 |
| C | ILE116 |
| C | SER119 |
| C | ALA136 |
| C | ASN138 |
| C | VAL140 |
| C | SER161 |
| C | HIS193 |
| C | ILE251 |
| C | HOH1429 |
| C | HOH1528 |
| site_id | AC4 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE NAD D 1355 |
| Chain | Residue |
| D | GLY29 |
| D | ALA30 |
| D | VAL31 |
| D | ASP52 |
| D | VAL53 |
| D | THR95 |
| D | ALA96 |
| D | GLY97 |
| D | ILE116 |
| D | SER119 |
| D | ALA136 |
| D | ALA137 |
| D | ASN138 |
| D | SER161 |
| D | LEU165 |
| D | HIS193 |
| D | ILE251 |
| D | HOH1387 |
| D | HOH1452 |
| D | HOH1467 |
| D | HOH1468 |
Functional Information from PROSITE/UniProt
| site_id | PS00064 |
| Number of Residues | 7 |
| Details | L_LDH L-lactate dehydrogenase active site. MGEHGDS |
| Chain | Residue | Details |
| A | MET190-SER196 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000305|PubMed:11807949, ECO:0000305|PubMed:1425707, ECO:0007744|PDB:1EZ4 |
| Chain | Residue | Details |
| A | HIS193 | |
| B | HIS193 | |
| C | HIS193 | |
| D | HIS193 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 28 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00488 |
| Chain | Residue | Details |
| A | VAL31 | |
| B | GLN100 | |
| B | ARG106 | |
| B | ASN138 | |
| B | ASP166 | |
| B | THR247 | |
| C | VAL31 | |
| C | TYR83 | |
| C | GLN100 | |
| C | ARG106 | |
| C | ASN138 | |
| A | TYR83 | |
| C | ASP166 | |
| C | THR247 | |
| D | VAL31 | |
| D | TYR83 | |
| D | GLN100 | |
| D | ARG106 | |
| D | ASN138 | |
| D | ASP166 | |
| D | THR247 | |
| A | GLN100 | |
| A | ARG106 | |
| A | ASN138 | |
| A | ASP166 | |
| A | THR247 | |
| B | VAL31 | |
| B | TYR83 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 24 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000269|PubMed:11807949, ECO:0007744|PDB:1EZ4 |
| Chain | Residue | Details |
| A | ASP52 | |
| B | SER119 | |
| B | ALA136 | |
| B | SER161 | |
| C | ASP52 | |
| C | ARG57 | |
| C | GLY97 | |
| C | SER119 | |
| C | ALA136 | |
| C | SER161 | |
| D | ASP52 | |
| A | ARG57 | |
| D | ARG57 | |
| D | GLY97 | |
| D | SER119 | |
| D | ALA136 | |
| D | SER161 | |
| A | GLY97 | |
| A | SER119 | |
| A | ALA136 | |
| A | SER161 | |
| B | ASP52 | |
| B | ARG57 | |
| B | GLY97 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000255|HAMAP-Rule:MF_00488 |
| Chain | Residue | Details |
| A | TYR238 | |
| B | TYR238 | |
| C | TYR238 | |
| D | TYR238 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| A | ASP166 | |
| A | HIS193 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| B | ASP166 | |
| B | HIS193 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| C | ASP166 | |
| C | HIS193 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| D | ASP166 | |
| D | HIS193 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| A | HIS193 | |
| A | ASP166 | |
| A | ARG169 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| B | HIS193 | |
| B | ASP166 | |
| B | ARG169 |
| site_id | CSA7 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| C | HIS193 | |
| C | ASP166 | |
| C | ARG169 |
| site_id | CSA8 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| D | HIS193 | |
| D | ASP166 | |
| D | ARG169 |
