1E89
ON THE MECHANISM OF CYANOGENESIS CATALYZED BY HYDROXYNITRILE LYASE FROM MANIHOT ESCULENTA. CRYSTAL STRUCTURE OF ACTIVE SITE MUTANT SER80ALA IN COMPLEX WITH ACETONE CYANOHYDRIN
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0047606 | molecular_function | (S)-hydroxynitrile lyase activity |
| A | 0052891 | molecular_function | aliphatic (S)-hydroxynitrile lyase activity |
| A | 0052892 | molecular_function | aromatic (S)-hydroxynitrile lyase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0016829 | molecular_function | lyase activity |
| B | 0047606 | molecular_function | (S)-hydroxynitrile lyase activity |
| B | 0052891 | molecular_function | aliphatic (S)-hydroxynitrile lyase activity |
| B | 0052892 | molecular_function | aromatic (S)-hydroxynitrile lyase activity |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 474 |
| Details | Domain: {"description":"AB hydrolase-1","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PubMed","id":"11173464","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11316882","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"11173464","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1DWO","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Site: {"description":"Increases basicity of active site His","evidences":[{"source":"PubMed","id":"11316882","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1dwo |
| Chain | Residue | Details |
| A | ALA80 | |
| A | HIS236 | |
| A | ASP208 | |
| A | THR11 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1dwo |
| Chain | Residue | Details |
| B | ALA80 | |
| B | HIS236 | |
| B | ASP208 | |
| B | THR11 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dwo |
| Chain | Residue | Details |
| A | ALA80 | |
| A | HIS236 | |
| A | ASP208 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dwo |
| Chain | Residue | Details |
| B | ALA80 | |
| B | HIS236 | |
| B | ASP208 |
