1E89

ON THE MECHANISM OF CYANOGENESIS CATALYZED BY HYDROXYNITRILE LYASE FROM MANIHOT ESCULENTA. CRYSTAL STRUCTURE OF ACTIVE SITE MUTANT SER80ALA IN COMPLEX WITH ACETONE CYANOHYDRIN

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016829	molecular_function	lyase activity
A	0047606	molecular_function	(S)-hydroxynitrile lyase activity
A	0052891	molecular_function	aliphatic (S)-hydroxynitrile lyase activity
A	0052892	molecular_function	aromatic (S)-hydroxynitrile lyase activity
B	0016829	molecular_function	lyase activity
B	0047606	molecular_function	(S)-hydroxynitrile lyase activity
B	0052891	molecular_function	aliphatic (S)-hydroxynitrile lyase activity
B	0052892	molecular_function	aromatic (S)-hydroxynitrile lyase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:11173464, ECO:0000305|PubMed:11316882

Chain	Residue	Details
A	CYS81
A	LYS237
B	CYS81
B	LYS237

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:11173464, ECO:0007744|PDB:1DWO

Chain	Residue	Details
A	ILE12
A	CYS81
A	ALA82
B	ILE12
B	CYS81
B	ALA82

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Increases basicity of active site His => ECO:0000305|PubMed:11316882

Chain	Residue	Details
A	LYS209
B	LYS209

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1dwo

Chain	Residue	Details
A	ALA80
A	HIS236
A	ASP208
A	THR11

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1dwo

Chain	Residue	Details
B	ALA80
B	HIS236
B	ASP208
B	THR11

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1dwo

Chain	Residue	Details
A	ALA80
A	HIS236
A	ASP208

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1dwo

Chain	Residue	Details
B	ALA80
B	HIS236
B	ASP208

---