1E7S

GDP 4-keto-6-deoxy-D-mannose epimerase reductase K140R

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005737	cellular_component	cytoplasm
A	0008152	biological_process	metabolic process
A	0009226	biological_process	nucleotide-sugar biosynthetic process
A	0009242	biological_process	colanic acid biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0016853	molecular_function	isomerase activity
A	0042351	biological_process	'de novo' GDP-L-fucose biosynthetic process
A	0042803	molecular_function	protein homodimerization activity
A	0050577	molecular_function	GDP-L-fucose synthase activity
A	0070401	molecular_function	NADP+ binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A1319

Chain	Residue
A	HIS11
A	ARG12
A	ARG20
A	THR35

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 A1320

Chain	Residue
A	PHE272
A	HOH2314
A	HOH2411
A	HOH2412
A	HOH2413
A	ARG152
A	PRO238
A	ARG254
A	VAL270
A	VAL271

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A1322

Chain	Residue
A	ARG209
A	LYS283
A	HOH2347
A	HOH2416
A	HOH2418

---

site_id	AC4
Number of Residues	32
Details	BINDING SITE FOR RESIDUE NAP A1317

Chain	Residue
A	GLY13
A	MET14
A	VAL15
A	ARG36
A	LEU39
A	ASN40
A	LEU41
A	LEU42
A	ALA63
A	ALA64
A	VAL66
A	GLY67
A	GLY68
A	ILE86
A	ASP171
A	ASN177
A	HIS179
A	UVW1318
A	HOH2119
A	HOH2236
A	HOH2239
A	HOH2394
A	HOH2395
A	HOH2396
A	HOH2397
A	HOH2398
A	HOH2399
A	HOH2400
A	HOH2401
A	HOH2402
A	HOH2403
A	HOH2404

---

site_id	AC5
Number of Residues	12
Details	BINDING SITE FOR RESIDUE UVW A1318

Chain	Residue
A	GLY67
A	GLY68
A	ILE69
A	VAL70
A	ALA71
A	SER178
A	LYS262
A	NAP1317
A	HOH2407
A	HOH2408
A	HOH2409
A	HOH2410

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE TRS A1321

Chain	Residue
A	ARG20
A	ARG21
A	HIS170
A	TRP311
A	HOH2414
A	HOH2415

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:11021971

Chain	Residue	Details
A	TYR136

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:11021971, ECO:0000269|PubMed:9817848, ECO:0000269|PubMed:9862812

Chain	Residue	Details
A	GLY10
A	ARG36
A	LEU105
A	ARG140
A	PRO163
A	HIS179

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305

Chain	Residue	Details
A	ARG187

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00956

Chain	Residue	Details
A	TRP202
A	ARG209
A	ASP278

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Important for catalytic activity

Chain	Residue	Details
A	SER107
A	CYS109

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	SITE: Lowers pKa of active site Tyr

Chain	Residue	Details
A	ARG140

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 227

Chain	Residue	Details
A	SER107	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, increase acidity, increase basicity, proton acceptor, proton donor
A	SER108	electrostatic stabiliser, hydrogen bond donor
A	CYS109	electrostatic stabiliser, hydrogen bond donor, proton acceptor
A	TYR136	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay
A	ARG140	electrostatic stabiliser, hydrogen bond donor, increase acidity, increase basicity
A	HIS179	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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