1E3L

P47H mutant of mouse class II alcohol dehydrogenase complex with NADH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001523	biological_process	retinoid metabolic process
A	0003960	molecular_function	quinone reductase (NADPH) activity
A	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
A	0004032	molecular_function	aldose reductase (NADPH) activity
A	0004745	molecular_function	all-trans-retinol dehydrogenase (NAD+) activity
A	0005503	molecular_function	all-trans retinal binding
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006066	biological_process	alcohol metabolic process
A	0006067	biological_process	ethanol metabolic process
A	0006081	biological_process	aldehyde metabolic process
A	0006629	biological_process	lipid metabolic process
A	0008270	molecular_function	zinc ion binding
A	0010430	biological_process	fatty acid omega-oxidation
A	0016491	molecular_function	oxidoreductase activity
A	0018479	molecular_function	benzaldehyde dehydrogenase (NAD+) activity
A	0019841	molecular_function	retinol binding
A	0042572	biological_process	retinol metabolic process
A	0044282	biological_process	small molecule catabolic process
A	0046164	biological_process	alcohol catabolic process
A	0046294	biological_process	formaldehyde catabolic process
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
A	0051903	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
A	1901661	biological_process	quinone metabolic process
B	0001523	biological_process	retinoid metabolic process
B	0003960	molecular_function	quinone reductase (NADPH) activity
B	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
B	0004032	molecular_function	aldose reductase (NADPH) activity
B	0004745	molecular_function	all-trans-retinol dehydrogenase (NAD+) activity
B	0005503	molecular_function	all-trans retinal binding
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006066	biological_process	alcohol metabolic process
B	0006067	biological_process	ethanol metabolic process
B	0006081	biological_process	aldehyde metabolic process
B	0006629	biological_process	lipid metabolic process
B	0008270	molecular_function	zinc ion binding
B	0010430	biological_process	fatty acid omega-oxidation
B	0016491	molecular_function	oxidoreductase activity
B	0018479	molecular_function	benzaldehyde dehydrogenase (NAD+) activity
B	0019841	molecular_function	retinol binding
B	0042572	biological_process	retinol metabolic process
B	0044282	biological_process	small molecule catabolic process
B	0046164	biological_process	alcohol catabolic process
B	0046294	biological_process	formaldehyde catabolic process
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
B	0051903	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
B	1901661	biological_process	quinone metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 380

Chain	Residue
A	CYS46
A	THR48
A	HIS67
A	CYS178
A	NAD377
A	HOH2093

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 381

Chain	Residue
A	CYS103
A	CYS111
A	GLY112
A	CYS97
A	LYS98
A	CYS100

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 380

Chain	Residue
B	CYS46
B	HIS67
B	CYS178
B	HOH2084

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN B 381

Chain	Residue
B	CYS97
B	LYS98
B	CYS100
B	CYS103
B	CYS111

---

site_id	AC5
Number of Residues	28
Details	BINDING SITE FOR RESIDUE NAD A 377

Chain	Residue
A	HIS47
A	THR48
A	CYS178
A	SER182
A	GLY203
A	LEU204
A	GLY205
A	CYS206
A	VAL207
A	ASP227
A	ILE228
A	ASN229
A	LYS232
A	CYS272
A	THR275
A	GLN277
A	THR278
A	VAL296
A	GLY297
A	ALA298
A	THR319
A	PHE320
A	PHE321
A	ARG371
A	ZN380
A	HOH2092
A	HOH2093
B	ASP362

---

site_id	AC6
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAD B 378

Chain	Residue
B	HIS47
B	THR48
B	CYS178
B	SER182
B	GLY203
B	GLY205
B	CYS206
B	VAL207
B	ASP227
B	ILE228
B	LYS232
B	CYS272
B	THR278
B	VAL296
B	GLY297
B	THR319
B	PHE320
B	PHE321
B	MET364
B	ARG371
B	HOH2048
B	HOH2083
B	HOH2084

---

Functional Information from PROSITE/UniProt

site_id	PS00059
Number of Residues	15
Details	ADH_ZINC Zinc-containing alcohol dehydrogenases signature. GHEcAGIvesvGpgV

Chain	Residue	Details
A	GLY66-VAL80

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	Binding site: {}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	26
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10970744","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1teh

Chain	Residue	Details
A	THR48
A	HIS47

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1teh

Chain	Residue	Details
B	THR48
B	HIS47

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 500

Chain	Residue	Details
A	CYS46	metal ligand
A	HIS47	steric role
A	THR48	proton shuttle (general acid/base)
A	HIS67	metal ligand
A	CYS178	metal ligand
A	SER182	proton shuttle (general acid/base)

---

site_id	MCSA2
Number of Residues	6
Details	M-CSA 500

Chain	Residue	Details
B	CYS46	metal ligand
B	HIS47	steric role
B	THR48	proton shuttle (general acid/base)
B	HIS67	metal ligand
B	CYS178	metal ligand
B	SER182	proton shuttle (general acid/base)

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